Anti-CSF-1-R antibody [EPR21885-161]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR21885-161] (AB221684)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling CSF-1-R with ab221684 at 1/50 dilution (8.66 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody used at 1/1000 dilution (2µg/ml) (Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5µg/ml)(Red). The Nuclear counterstain was DAPI (Blue). Confocal image showing membranous and cytoplasmic staining in J774A.1 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• WB

Supplier Data

Western blot - Anti-CSF-1-R antibody [EPR21885-161] (AB221684)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-4 : 3 minutes; Lane 5 : 32 seconds.

The molecular mass observed is consistent with the literature (PMID 21610095; PMID 20436485; PMID 24362524; PMID : 26066800).

All lanes:

Western blot - Anti-CSF-1-R antibody [EPR21885-161] (ab221684) at 1/1000 dilution

Lane 1:

Rat lung lysate at 20 µg

Lane 2:

Mouse spleen lysate at 20 µg

Lane 3:

Mouse placenta lysate at 20 µg

Lane 4:

J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 10 µg

Lane 5:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Lane 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lane 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 5:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 108 kDa

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