Anti-CSF-1-R antibody [EPR23529-26] (ab254357) is a rabbit monoclonal antibody that is used to detect CSF-1-R in Western Blot, IP, IHC-P, ICC/IF. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (AB254357), expandable thumbnail](https://content.abcam.com/products/images/csf-1-r-antibody-epr23529-26-ab254357--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113061.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (AB254357)")
![Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] (AB254357), expandable thumbnail](https://content.abcam.com/products/images/csf-1-r-antibody-epr23529-26-ab254357--immunocytochemistry-immunofluorescence-img126173.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] (AB254357)")
![Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] (AB254357), expandable thumbnail](https://content.abcam.com/products/images/csf-1-r-antibody-epr23529-26-ab254357--immunoprecipitation-img11515.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] (AB254357)")
![Western blot - Anti-CSF-1-R antibody [EPR23529-26] (AB254357), expandable thumbnail](https://content.abcam.com/products/images/csf-1-r-antibody-epr23529-26-ab254357--western-blot-img1131.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CSF-1-R antibody [EPR23529-26] (AB254357)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (AB254357), expandable thumbnail](https://content.abcam.com/products/images/csf-1-r-antibody-epr23529-26-ab254357--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34162.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (AB254357)")
Publications
Filter by
- Nature communications 12:52892021Capillary-associated microglia regulate vascular structure and function through PANX1-P2RY12 coupling in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKanchan Bisht,Kenneth A Okojie,Kaushik Sharma,Dennis H Lentferink,Yu-Yo Sun,Hong-Ru Chen,Joseph O Uweru,Saipranusha Amancherla,Zainab Calcuttawala,Antony Brayan Campos-Salazar,Bruce Corliss,Lara Jabbour,Jordan Benderoth,Bria Friestad,William A Mills,Brant E Isakson,Marie-Ève Tremblay,Chia-Yi Kuan,Ukpong B EyoPubMed 34489419
- Mediators of inflammation 2020:87961032020Combinational Pretreatment of Colony-Stimulating Factor 1 Receptor Inhibitor and Triptolide Upregulates BDNF-Akt and Autophagic Pathways to Improve Cerebral Ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxue Du et. al.PubMed 33192177
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (By similarity).
Alternative names
CD115, Csfmr, Fms, Csf1r, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R
Recommended products
Anti-CSF-1-R antibody [EPR23529-26] (ab254357) is a rabbit monoclonal antibody that is used to detect CSF-1-R in Western Blot, IP, IHC-P, ICC/IF. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR23529-26
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CSF-1-R antibody [EPR23529-26] (ab254357) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Mouse samples.
What is the molecular weight of CSF-1-R?
Anti-CSF-1-R [EPR23529-26] (ab254357) specifically detects a band for CSF-1-R (UniProt: P09581) at a molecular weight of 108kDa.
Recommended positive controls
WB: RAW 264.7, J774A.1 and Mouse spleen, Mouse placenta lysates.IHC-P: Mouse spleen, Mouse liver tissues.ICC/IF: RAW 264.7 cells. IP: RAW 264.7 cells.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products
We have a range of other formats of antibody clone [EPR23529-26] also available for your convenience:
ab254357, Carrier free - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free ab272049, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-CSF-1-R antibody [EPR23529-26] ab275171
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357), expandable thumbnail](https://content.abcam.com/products/images/csf-1-r-antibody-epr23529-26-ab254357--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113061.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on macrophages in mouse spleen. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] (ab254357), expandable thumbnail](https://content.abcam.com/products/images/csf-1-r-antibody-epr23529-26-ab254357--immunocytochemistry-immunofluorescence-img126173.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)")
Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW264.7 cells labelling CSF-1-R with ab254357 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] (ab254357), expandable thumbnail](https://content.abcam.com/products/images/csf-1-r-antibody-epr23529-26-ab254357--immunoprecipitation-img11515.jpg "Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)")
Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
CSF-1-R was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab254357 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254357 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug
Lane 2: ab254357 IP in RAW 264.7 whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab254357 in RAW 264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 128 seconds.
All lanes: Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
Predicted band size: 108 kDa
![Western blot - Anti-CSF-1-R antibody [EPR23529-26] (ab254357), expandable thumbnail](https://content.abcam.com/products/images/csf-1-r-antibody-epr23529-26-ab254357--western-blot-img1131.jpg "Western blot - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)")
Western blot - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1-2: 70 seconds; Lanes 3-4: 3 minutes.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:14673177)
This blot was developed using a higher sensitivity ECL substrate.All lanes: Western blot - Anti-CSF-1-R antibody [EPR23529-26] (ab254357) at 1/1000 dilution
Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Mouse placenta tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 108 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357), expandable thumbnail](https://content.abcam.com/products/images/csf-1-r-antibody-epr23529-26-ab254357--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34162.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on Kupffer cells in mouse liver. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com