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Rabbit Recombinant Monoclonal CSF-1-R antibody. Carrier free. Suitable for mIHC, WB, IHC-P, ICC/IF, IP and reacts with Human samples.

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Images

Western blot - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (AB313649), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (AB313649), expandable thumbnail
  • Immunoprecipitation - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (AB313649), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (AB313649), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (AB313649), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCWBIHC-PICC/IFFlow CytIP
Human
Tested
Tested
Tested
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CSF-1-R antibody. Carrier free. Suitable for mIHC, WB, IHC-P, ICC/IF, IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28407-22
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649), expandable thumbnail

    Western blot - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649)

    CSF-1-R Western blot staining using rabbit Anti-CSF-1-R antibody

    This data was developed using Anti-CSF-1-R antibody [EPR28407-22] ab313648, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Raji, HDLM-2 (PMID: 26066800).

    The expression molecular weight observed is consistent with what has been described in the literature (PMID: 14673177).

    In lanes 1-3, the lysates were stored at -80°C prior to Western Blotting. The bands beneath the target band (140 kDa) are likey to be degradation products. In lanes 4-5, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CSF-1-R antibody [EPR28407-22] (Anti-CSF-1-R antibody [EPR28407-22] ab313648) at 1/1000 dilution

    Lanes 1 and 4: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lanes 2 and 5: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 3: HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 190 kDa, 140 kDa

    Exposure time: 180s

  • Multiplex immunohistochemistry - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649)

    CSF-1-R Multiplex immunohistochemistry staining of Human tonsil tissue using rabbit Anti-CSF-1-R antibody

    This data was developed using Anti-CSF-1-R antibody [EPR28407-22] ab313648, the same antibody clone in a different buffer formulation.

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining CARD9 with Anti-CARD9 antibody [EPR28960-64] ab322105 at a 1/100 (4.99 µg/ml) dilution, Anti-CSF-1-R antibody [EPR28407-22] ab313648 anti-CSF-1-R used at 1/100 (5.26 µg/ml) dilution.

    Panel A: merged staining of anti-CARD9 (green; Opal™520) and anti-CSF-1-R (magenta; Opal™570) on human tonsil.

    Panel B: anti-CARD9 staining immune cells in human tonsil.

    Panel C: anti-CSF-1-R staining macrophages and monocytes in human tonsil.

    Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-CARD9 antibody [EPR28960-64] ab322105 and Anti-CSF-1-R antibody [EPR28407-22] ab313648 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649), expandable thumbnail

    Immunoprecipitation - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649)

    MCSF Receptor was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with Anti-CSF-1-R antibody [EPR28407-22] ab313648 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using Anti-CSF-1-R antibody [EPR28407-22] ab313648 at 1/1000 dilution. Secondary antibody details (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate 20 μg.

    Lane 2: Anti-CSF-1-R antibody [EPR28407-22] ab313648 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CSF-1-R antibody [EPR28407-22] ab313648 in THP-1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CSF-1-R antibody [EPR28407-22] ab313648).

    Lysates were freshly made and used for immunoprecipitation immediately to minimize protein degradation.

    All lanes: Immunoprecipitation - Anti-CSF-1-R antibody [EPR28407-22] (Anti-CSF-1-R antibody [EPR28407-22] ab313648) at 1/1000 dilution

    Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 2: Anti-CSF-1-R antibody [EPR28407-22] ab313648 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate.

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Predicted band size: 108 kDa

    Observed band size: 180 kDa

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649)

    This data was developed using Anti-CSF-1-R antibody [EPR28407-22] ab313648, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling MCSF Receptor with Anti-CSF-1-R antibody [EPR28407-22] ab313648 at 1/50 (10.52 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in THP-1 cell line.Negative control: Raji.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649)

    This data was developed using Anti-CSF-1-R antibody [EPR28407-22] ab313648, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human colorectal car tissue labeling MCSF Receptor with Anti-CSF-1-R antibody [EPR28407-22] ab313648 at 1/100 (5.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells of human colorectal carcinoma.The section was incubated with Anti-CSF-1-R antibody [EPR28407-22] ab313648 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649)

    This data was developed using Anti-CSF-1-R antibody [EPR28407-22] ab313648, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MCSF Receptor with Anti-CSF-1-R antibody [EPR28407-22] ab313648 at 1/100 (5.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells of human colon.The section was incubated with Anti-CSF-1-R antibody [EPR28407-22] ab313648 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR28407-22] - BSA and Azide free (ab313649)

    This data was developed using Anti-CSF-1-R antibody [EPR28407-22] ab313648, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MCSF Receptor with Anti-CSF-1-R antibody [EPR28407-22] ab313648 at 1/100 (5.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil (PMID: 26066800)The section was incubated with Anti-CSF-1-R antibody [EPR28407-22] ab313648 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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