Anti-CSF-1-R antibody [EPR29702-379]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CSF-1-R antibody [EPR29702-379] (AB319160)

Flow cytometric analysis of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) (Right) / NIH/3T3 (mouse embryonic fibroblast) (Left) cells labelling CSF-1-R with ab319160 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

The experiment was conducted on freshly cultured cells and cells were stained at low temperatures (2 - 8°C) on ice to preserve the surface expression of CD115, since CD115 is prone to rapid internalization under ambient room temperature conditions (PMID : 21466808).
Negative control : NIH/3T3 (PMID : 36555673).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR29702-379] (AB319160)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling CSF-1-R with ab319160 at 1/100 (5.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing membranous with cytoplasmic staining in RAW 264.7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression : NIH/3T3 (PMID : 36555673).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CSF-1-R antibody [EPR29702-379] (AB319160)

Flow cytometric analysis of Mouse bone marrow cells labelling CSF-1-R with ab319160 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

The experiment was conducted on freshly cultured cells and cells were stained at low temperatures (2 - 8°C) on ice to preserve the surface expression of CD115, since CD115 is prone to rapid internalization under ambient room temperature conditions (PMID : 21466808).
Cells were co-stained with anti mouse CD11b conjugated to Brilliant Violet 510.
Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CSF-1-R antibody [EPR29702-379] (AB319160)

Flow cytometric analysis of Mouse bone marrow cells labelling CSF-1-R with ab319160 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

The experiment was conducted on freshly cultured cells and cells were stained at low temperatures (2 - 8°C) on ice to preserve the surface expression of CD115, since CD115 is prone to rapid internalization under ambient room temperature conditions (PMID : 21466808).
Cells were co-stained with anti mouse CD3 conjugated to Alexa Fluor®647.
Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CSF-1-R antibody [EPR29702-379] (AB319160)

Flow cytometric analysis of Mouse bone marrow cells labelling CSF-1-R with ab319160 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

The experiment was conducted on freshly cultured cells and cells were stained at low temperatures (2 - 8°C) on ice to preserve the surface expression of CD115, since CD115 is prone to rapid internalization under ambient room temperature conditions (PMID : 21466808).
Cells were co-stained with anti mouse B220 conjugated to PE-Cy5.5 and anti mouse F4/80 conjugated to Alexa Fluor®647.
Gated on viable B220 negative cells.

• Dot

Supplier Data

Dot Blot - Anti-CSF-1-R antibody [EPR29702-379] (AB319160)

Dot blot analysis of CSF-1-R using ab319160 at 1 : 1000 (0.51 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged mouse CSF-1-R recombinant protein fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-CSF-1-R antibody [EPR29702-379] (ab319160) at 1/1000 dilution

All lanes:

His-tagged mouse CSF-1-R recombinant protein fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s