Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (AB271304)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling CSF1R with ab271294 at 1/50 dilution (24.16μg/ml), followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous and weak cytoplasmic staining in RAW 264.7 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab271294 at a 1/50 dilution (24.16μg/ml) followed by ab150080 at a 1/200 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271294).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (AB271304)

Immunohistochemistry analysis of paraffin-embedded mouse lymph node tissue section labelling CSF-1-R with ab271294 at 1/200 dilution. The section was incubated with ab271294 at +4°C overnight. Goat Anti-Rat IgG H&L (HRP polymer) (ab214882) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Positive staining on the macrophages in mouse lymph node. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271294).

• WB

Lab

Western blot - Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (AB271304)

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID : 14673177).
This blot was developed using a higher sensitivity ECL substrate.

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271294).

All lanes:

Western blot - Anti-CSF-1-R antibody [YEYE311] (<a href='/en-us/products/primary-antibodies/csf-1-r-antibody-yeye311-ab271294'>ab271294</a>) at 1.208 µg/mL

Lane 1:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 2:

J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 108 kDa

false

Exposure time: 3min