Anti-CSL4 antibody [EPR13525] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13525] - BSA and Azide free (AB250393)

This data was developed using ab181167, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling CSL4 with ab181167 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CSL4 antibody [EPR13525] - BSA and Azide free (AB250393)

This data was developed using ab181167, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling CSL4 with ab181167 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CSL4 antibody [EPR13525] - BSA and Azide free (AB250393)

This data was developed using ab181167, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of K562 cells using ab181167 at a 1/90 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-CSL4 antibody [EPR13525] - BSA and Azide free (AB250393)

This data was developed using ab181167, the same antibody clone in a different buffer formulation.

Detection of Human CSL4 by Western Blot of Immunoprecipitate. 293T whole cell lysate (lane 1) and a negative control (lane 2) loaded, ab181167 used for blotting immunoprecipitated CSL4 at a 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary used at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-CSL4 antibody [EPR13525] (<a href='/en-us/products/primary-antibodies/csl4-antibody-epr13525-ab181167'>ab181167</a>)

Predicted band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-CSL4 antibody [EPR13525] - BSA and Azide free (AB250393)

This data was developed using ab181167, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CSL4 antibody [EPR13525] (<a href='/en-us/products/primary-antibodies/csl4-antibody-epr13525-ab181167'>ab181167</a>) at 1/2000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

false

• WB

Lab

Western blot - Anti-CSL4 antibody [EPR13525] - BSA and Azide free (AB250393)

All lanes:

Western blot - Anti-CSL4 antibody [EPR13525] (<a href='/en-us/products/primary-antibodies/csl4-antibody-epr13525-ab181167'>ab181167</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

EXOSC1 CRISPR-Cas9 edited HeLa cell lysate at 20 µg

Lane 3:

PC-12 cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 21 kDa

Observed band size: 22 kDa

false