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Rabbit Recombinant Monoclonal CSL4 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-CSL4 antibody [EPR13526] (AB181108), expandable thumbnail
  • Immunoprecipitation - Anti-CSL4 antibody [EPR13526] (AB181108), expandable thumbnail
  • Western blot - Anti-CSL4 antibody [EPR13526] (AB181108), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] (AB181108), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CSL4 antibody [EPR13526] (AB181108), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/60
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC1 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC6 and EXOSC8.

Alternative names

CSL4, CGI-108, EXOSC1, Exosome complex component CSL4, Exosome component 1

Recommended products

Rabbit Recombinant Monoclonal CSL4 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13526
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-CSL4 antibody [EPR13526] (ab181108), expandable thumbnail

    Western blot - Anti-CSL4 antibody [EPR13526] (ab181108)

    False colour image of Western blot: Anti-CSL4 antibody [EPR13526] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab181108 was shown to bind specifically to CSL4. A band was observed at 22 kDa in wild-type HeLa cell lysates with no signal observed at this size in EXOSC1 CRISPR-Cas9 edited cell line ab265194 (EXOSC1 CRISPR-Cas9 edited cell lysate ab257945). The band observed in the CRISPR-Cas9 edited lysate lane above 22 kDa is likely to represent CSL4 with an insertion. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and EXOSC1 CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-CSL4 antibody [EPR13526] (ab181108) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: EXOSC1 CRISPR-Cas9 edited HeLa cell lysate at 20 µg

    Lane 3: PC-12 cell lysate at 20 µg

    Lane 4: SH-SY5Y cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 21 kDa

    Observed band size: 22 kDa

  • Immunoprecipitation - Anti-CSL4 antibody [EPR13526] (ab181108), expandable thumbnail

    Immunoprecipitation - Anti-CSL4 antibody [EPR13526] (ab181108)

    Western blot analysis of CSL4 in immunoprecipitation pellets from Raji lysate. ab181108 used at 1/60 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Lane 2 shows negative control.

    All lanes: Immunoprecipitation - Anti-CSL4 antibody [EPR13526] (ab181108)

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Western blot - Anti-CSL4 antibody [EPR13526] (ab181108), expandable thumbnail

    Western blot - Anti-CSL4 antibody [EPR13526] (ab181108)

    All lanes: Western blot - Anti-CSL4 antibody [EPR13526] (ab181108) at 1/2000 dilution

    Lane 1: K562 cell lysate at 10 µg

    Lane 2: C6 cell lysate at 10 µg

    Lane 3: Raw264.7 cell lysate at 10 µg

    Lane 4: PC-12 cell lysate at 10 µg

    Lane 5: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] (ab181108), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] (ab181108)

    Immunohistochemical analysis of paraffin-embedded Human cervix tissue labeling CSL4 with ab181108 at 1/100 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CSL4 antibody [EPR13526] (ab181108), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CSL4 antibody [EPR13526] (ab181108)

    Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling CSL4 with ab181108 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488), Conterstained with DAPI blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] (ab181108), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] (ab181108)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CSL4 with ab181108 at 1/100 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-CSL4 antibody [EPR13526] (ab181108), expandable thumbnail

    Western blot - Anti-CSL4 antibody [EPR13526] (ab181108)

    All lanes: Western blot - Anti-CSL4 antibody [EPR13526] (ab181108) at 1/10000 dilution

    Lane 1: Raji cell lysate at 10 µg

    Lane 2: 293 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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