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AB181108

Anti-CSL4 antibody [EPR13526]

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(1 Publication)

Rabbit Recombinant Monoclonal CSL4 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CSL4, CGI-108, EXOSC1, Exosome complex component CSL4, Exosome component 1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] (AB181108)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] (AB181108)

Immunohistochemical analysis of paraffin-embedded Human cervix tissue labeling CSL4 with ab181108 at 1/100 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] (AB181108)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13526] (AB181108)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CSL4 with ab181108 at 1/100 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CSL4 antibody [EPR13526] (AB181108)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CSL4 antibody [EPR13526] (AB181108)

Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling CSL4 with ab181108 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) Conterstained with DAPI blue.

Immunoprecipitation - Anti-CSL4 antibody [EPR13526] (AB181108)
  • IP

Supplier Data

Immunoprecipitation - Anti-CSL4 antibody [EPR13526] (AB181108)

Western blot analysis of CSL4 in immunoprecipitation pellets from Raji lysate. ab181108 used at 1/60 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Lane 2 shows negative control.

All lanes:

Immunoprecipitation - Anti-CSL4 antibody [EPR13526] (ab181108)

Predicted band size: 21 kDa

Observed band size: 21 kDa

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Western blot - Anti-CSL4 antibody [EPR13526] (AB181108)
  • WB

Supplier Data

Western blot - Anti-CSL4 antibody [EPR13526] (AB181108)

All lanes:

Western blot - Anti-CSL4 antibody [EPR13526] (ab181108) at 1/2000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

C6 cell lysate at 10 µg

Lane 3:

Raw264.7 cell lysate at 10 µg

Lane 4:

PC-12 cell lysate at 10 µg

Lane 5:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

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Western blot - Anti-CSL4 antibody [EPR13526] (AB181108)
  • WB

Lab

Western blot - Anti-CSL4 antibody [EPR13526] (AB181108)

False colour image of Western blot : Anti-CSL4 antibody [EPR13526] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab181108 was shown to bind specifically to CSL4. A band was observed at 22 kDa in wild-type HeLa cell lysates with no signal observed at this size in EXOSC1 CRISPR-Cas9 edited cell line ab265194 (EXOSC1 CRISPR-Cas9 edited cell lysate ab257945). The band observed in the CRISPR-Cas9 edited lysate lane above 22 kDa is likely to represent CSL4 with an insertion. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and EXOSC1 CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-CSL4 antibody [EPR13526] (ab181108) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

EXOSC1 CRISPR-Cas9 edited HeLa cell lysate at 20 µg

Lane 3:

PC-12 cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 21 kDa

Observed band size: 22 kDa

false

Western blot - Anti-CSL4 antibody [EPR13526] (AB181108)
  • WB

Supplier Data

Western blot - Anti-CSL4 antibody [EPR13526] (AB181108)

All lanes:

Western blot - Anti-CSL4 antibody [EPR13526] (ab181108) at 1/10000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

293 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

  • Carrier free

    Anti-CSL4 antibody [EPR13526] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13526

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC1 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC6 and EXOSC8.
See full target information EXOSC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 14: PubMed40146629

2025

CDK-mediated phosphorylation of PNKP is required for end-processing of single-strand DNA gaps on Okazaki fragments and genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Kaima Tsukada,Rikiya Imamura,Tomoko Miyake,Kotaro Saikawa,Mizuki Saito,Naoya Kase,Lingyan Fu,Masamichi Ishiai,Yoshihisa Matsumoto,Mikio Shimada
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com