AB10413

Anti-CSN1 antibody

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(8 Publications)

Rabbit Polyclonal CSN1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human GPS1 aa 400 to C-terminus.

View Alternative Names

COPS1, CSN1, GPS1, COP9 signalosome complex subunit 1, SGN1, Signalosome subunit 1, G protein pathway suppressor 1, JAB1-containing signalosome subunit 1, Protein MFH, GPS-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody (AB10413)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody (AB10413)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CSN1 with ab10413 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Western blot - Anti-CSN1 antibody (AB10413)

Detection of Human CSN1 by Western Blot. Samples : Whole cell lysate (50 mcg – E; 10 mcg – T) from mock transfected (E) or CSN1 transfected (T) HEK293T cells.

All lanes:

Western blot - Anti-CSN1 antibody (ab10413)

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human GPS1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13098

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein- and mitogen-activated protein kinase-mediated signal transduction.

See full target information GPS1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39273640

2024

MicroRNA-27a-5p Downregulates Expression of Proinflammatory Cytokines in Lipopolysaccharide-Stimulated Human Dental Pulp Cells via the NF-κB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shihan Wang,Nobuyuki Kawashima,Peifeng Han,Keisuke Sunada-Nara,Ziniu Yu,Kento Tazawa,Mayuko Fujii,Thoai Quoc Kieu,Takashi Okiji

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2405346 PubMed39136073

2024

Th1/Th2 Imbalance in Peripheral Blood Echoes Microglia State Dynamics in CNS During TLE Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Yuanxia Wu,Jing Chen,Qiong Zhang,Yunyi Liu,Hongyu Long,Jianhua Yu,Qian Wu,Li Feng

Biomolecules 13: PubMed37759725

2023

Neuroactive Steroid-Gut Microbiota Interaction in T2DM Diabetic Encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Diviccaro,Lucia Cioffi,Rocco Piazza,Donatella Caruso,Roberto Cosimo Melcangi,Silvia Giatti

Nutrients 14: PubMed36364920

2022

Aging Increases Susceptibility to Develop Cardiac Hypertrophy following High Sugar Consumption.

Applications

Unspecified application

Species

Unspecified reactive species

Ana P Valencia,Jeremy A Whitson,Shari Wang,Leon Nguyen,Laura J den Hartigh,Peter S Rabinovitch,David J Marcinek

Journal of bone and mineral research : the officia 34:1646-1659 PubMed30908713

2019

Compromised Exercise Capacity and Mitochondrial Dysfunction in the Osteogenesis Imperfecta Murine (oim) Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria L Gremminger,Youngjae Jeong,Rory P Cunningham,Grace M Meers,R Scott Rector,Charlotte L Phillips

Journal of cellular physiology 234:21331-21341 PubMed31042008

2019

Anti-inflammatory roles of microRNA 21 in lipopolysaccharide-stimulated human dental pulp cells.

Applications

Unspecified application

Species

Unspecified reactive species

Keisuke Nara,Nobuyuki Kawashima,Sonoko Noda,Mayuko Fujii,Kentaro Hashimoto,Kento Tazawa,Takashi Okiji

Biology of sex differences 9:6 PubMed29351809

2018

Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features.

Applications

Unspecified application

Species

Unspecified reactive species

Marzia Pesaresi,Silvia Giatti,Roberto Spezzano,Simone Romano,Silvia Diviccaro,Tiziana Borsello,Nico Mitro,Donatella Caruso,Luis Miguel Garcia-Segura,Roberto Cosimo Melcangi

The Journal of biological chemistry 281:17457-65 PubMed16624822

2006

Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2.

Applications

Unspecified application

Species

Unspecified reactive species

Wonkyung Oh,Eun-Woo Lee,Young Hoon Sung,Mi-Ran Yang,Jaewang Ghim,Han-Woong Lee,Jaewhan Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CSN1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody (AB10413)

Western blot - Anti-CSN1 antibody (AB10413)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

Product promise