Anti-CSN1 antibody [EP15642-22] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] - BSA and Azide free (AB284850)

This data was developed using ab194359, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CSN1 with ab194359 at 1/300 (6μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution  (ab97051) and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CSN1 antibody [EP15642-22] - BSA and Azide free (AB284850)

This data was developed using ab194359, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CSN1 with ab194359 at 1/150 dilution (12 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] - BSA and Azide free (AB284850)

This data was developed using ab194359, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CSN1 with ab194359 at 1/300 (6μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution (ab97051) and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-CSN1 antibody [EP15642-22] - BSA and Azide free (AB284850)

This data was developed using ab194359, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CSN1 antibody [EP15642-22] (<a href='/en-us/products/primary-antibodies/csn1-antibody-ep15642-22-ab194359'>ab194359</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 56 kDa

false

• WB

Supplier Data

Western blot - Anti-CSN1 antibody [EP15642-22] - BSA and Azide free (AB284850)

This data was developed using ab194359, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CSN1 antibody [EP15642-22] (<a href='/en-us/products/primary-antibodies/csn1-antibody-ep15642-22-ab194359'>ab194359</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 56 kDa

false