AB124779

Anti-CSN8 antibody [EPR5139]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal CSN8 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

CSN8, COPS8, COP9 signalosome complex subunit 8, SGN8, Signalosome subunit 8, COP9 homolog, JAB1-containing signalosome subunit 8, hCOP9

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN8 antibody [EPR5139] (AB124779)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN8 antibody [EPR5139] (AB124779)

ab124779 at 1/100 staining CSN8 in formalin fixed paraffin embedded Human brain tissue using immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CSN8 antibody [EPR5139] (AB124779)

All lanes:

Western blot - Anti-CSN8 antibody [EPR5139] (ab124779) at 1/1000 dilution

Lane 1:

Fetal liver cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

SH SY5Y cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 23 kDa

Observed band size: 21 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5139

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Antigen retrieval is recommended.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.

See full target information COPS8

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 26:1344-1366 PubMed39915298

2025

HSC70 coordinates COP9 signalosome and SCF ubiquitin ligase activity to enable a prompt stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Shunsuke Nishimura,Hidetaka Kioka,Shan Ding,Hideyuki Hakui,Haruki Shinomiya,Kazuya Tanabe,Tatsuro Hitsumoto,Ken Matsuoka,Hisakazu Kato,Osamu Tsukamoto,Yoshihiro Asano,Seiji Takashima,Radoslav I Enchev,Yasushi Sakata

The FEBS journal 282:3737-57 PubMed26175140

2015

Identification of novel dipeptidyl peptidase 9 substrates by two-dimensional differential in-gel electrophoresis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Sadiqa Maqsudi,Adam Rainczuk,Nadine Duffield,Josie Lawrence,Fiona M Keane,Daniela Justa-Schuch,Ruth Geiss-Friedlander,Mark D Gorrell,Andrew N Stephens

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CSN8 antibody [EPR5139]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN8 antibody [EPR5139] (AB124779)

Western blot - Anti-CSN8 antibody [EPR5139] (AB124779)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Product promise