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AB108296

Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)]

4

(2 Reviews)

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(24 Publications)

Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) is a rabbit monoclonal antibody detecting CSNK1A1 + CSNK1A1L in Western Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

Casein kinase I isoform alpha, CKI-alpha, CK1, CSNK1A1, Casein kinase I isoform alpha-like, CKI-alpha-like, CK1, CSNK1A1L

6 Images
Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)
  • WB

Lab

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 15 µg

Lane 2:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 39 kDa

false

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)
  • WB

Unknown

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)

All lanes:

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HT-1376 cell lysate at 10 µg

Predicted band size: 39 kDa

Observed band size: 34 kDa

false

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)
  • WB

Lab

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)

ab108296 was shown to react with CSNK1A1 in wild-type HCT116 cells in Western blot with loss of signal observed in a CSNK1A1 siRNA knockdown cell line. Cell lysates from wild-type HCT116 transfected with either scrambled siRNA or CSNK1A1 siRNA were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab108296 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution

Lane 1:

Wild-type HCT116 transfected with scrambled siRNA control lysate at 50 µg

Lane 2:

HCT116 transfected with siRNA specifically targeting CSNK1A1 cell lysate at 50 µg

false

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)
  • WB

Lab

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/2000 dilution

All lanes:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 116 kDa,129 kDa,15 kDa,16 kDa,29 kDa,35 kDa,39 kDa,47 kDa,48 kDa,51 kDa,53 kDa,56 kDa,60 kDa,71 kDa,83 kDa,87 kDa,95 kDa

Observed band size: 117 kDa,140 kDa,15 kDa,150 kDa,16 kDa,35 kDa,45 kDa,45-65 kDa,50 kDa,51 kDa,52 kDa,58 kDa,60 kDa,68 kDa,71 kDa,95 kDa

false

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)
  • WB

Lab

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution

Lane 1:

Rat brain lysates at 15 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 39 kDa

false

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)
  • WB

Unknown

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (AB108296)

All lanes:

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution

Lane 1:

N-GST tagged Human CSNK1A1L full-length (aa1 to 337) recombinant protein 10ng

Lane 2:

N-GST tagged Human CSNK1A1 full-length (aa1 to 337) recombinant protein 10ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 39 kDa

false

  • Carrier free

    Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1961(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000 - 1/10000</strong></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000 - 1/10000</strong></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000 - 1/10000</strong></p>" } } }
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Product details

What is this antibody validated in?
Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of CSNK1A1 + CSNK1A1L?
Anti-CSNK1A1 + CSNK1A1L [EPR1961(2)] (ab108296) specifically detects a band for CSNK1A1 + CSNK1A1L (UniProt: P48729) at a molecular weight of 39kDa.

Trusted by the scientific community
Anti-CSNK1A1 + CSNK1A1L [EPR1961(2)] (ab108296) was first used in a scientific publication in 2011 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR1961(2)] also available for your convenience: ab108296, Carrier free - ab242383

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (PubMed : 11955436, PubMed : 1409656, PubMed : 18305108, PubMed : 23902688). It can phosphorylate a large number of proteins (PubMed : 11955436, PubMed : 1409656, PubMed : 18305108, PubMed : 23902688). Participates in Wnt signaling (PubMed : 11955436). Phosphorylates CTNNB1 at 'Ser-45' (PubMed : 11955436). May phosphorylate PER1 and PER2 (By similarity). May play a role in segregating chromosomes during mitosis (PubMed : 1409656). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed : 23902688). Acts as a positive regulator of mTORC1 and mTORC2 signaling in response to nutrients by mediating phosphorylation of DEPTOR inhibitor (PubMed : 22017875, PubMed : 22017877). Acts as an inhibitor of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3 (By similarity).
See full target information CSNK1A1

Additional targets

Casein kinase I isoform alpha-like
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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:2981-3006 PubMed41050081

2025

Discovery and Characterization of Novel IKZF1/3 Glue Degraders against Multiple Hematological Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Wei,Pengli Wei,Yalei Wang,Yaqiu Mao,Jian Yan,Xiaotong Hu,Zhenze Qi,Xu Cai,Changkai Jia,Zhiyuan Zhao,Bingkun Li,Min Qiao,Yaxin Zou,Tingting Yang,Shiyang Sun,Xuesong Feng,Pengyun Li,Hongzhou Shang,Zhibing Zheng

Leukemia 39:2163-2173 PubMed40670672

2025

Targeting of IRAK4 and GSPT1 enhances therapeutic efficacy in AML via c-Myc destabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Eric J Vick,Aishlin Hassan,Kwangmin Choi,Joshua Bennett,Tomoya Muto,Courtnee A Clough,Ashley E Culver-Cochran,Kathleen Hueneman,Lyndsey C Bolanos,Mark Wunderlich,Xiaohu Zhang,Crystal McKnight,Michele Ceribelli,David Holland,Carleen Klumpp-Thomas,Kenneth D Greis,Craig J Thomas,Daniel T Starczynowski

F1000Research 13:1055 PubMed40028451

2025

A guide to selecting high-performing antibodies for CSNK1A1 (UniProt ID: P48729) for use in western blot, immunoprecipitation, and immunofluorescence.

Applications

WB, ICC

Species

Human, Human

Riham Ayoubi,Charles Alende,Maryam Fotouhi,Vera Ruíz Moleón,Sara González Bolívar,Kathleen Southern,Carl Laflamme

Biochemical and biophysical research communications 723:150189 PubMed38852281

2024

Exploring the role of casein kinase 1α splice variants across cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo A Meléndez,Daniel T Wynn,Siva Bharath Merugu,Prerna Singh,Kenton P Kaplan,David J Robbins

Nature communications 14:4683 PubMed37596276

2023

Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Yamanaka,Hirotake Furihata,Yuta Yanagihara,Akihito Taya,Takato Nagasaka,Mai Usui,Koya Nagaoka,Yuki Shoya,Kohei Nishino,Shuhei Yoshida,Hidetaka Kosako,Masaru Tanokura,Takuya Miyakawa,Yuuki Imai,Norio Shibata,Tatsuya Sawasaki

The Journal of cell biology 221: PubMed36342472

2022

Dishevelled phase separation promotes Wnt signalosome assembly and destruction complex disassembly.

Applications

Unspecified application

Species

Unspecified reactive species

Kexin Kang,Qiaoni Shi,Xu Wang,Ye-Guang Chen

Nature cancer 3:595-613 PubMed35534777

2022

IKAROS and MENIN coordinate therapeutically actionable leukemogenic gene expression in MLL-r acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon J Aubrey,Jevon A Cutler,Wallace Bourgeois,Katherine A Donovan,Shengqing Gu,Charlie Hatton,Sarah Perlee,Florian Perner,Homa Rahnamoun,Alexandra C P Theall,Jill A Henrich,Qian Zhu,Radosław P Nowak,Young Joon Kim,Salma Parvin,Anjali Cremer,Sarah Naomi Olsen,Nicholas A Eleuteri,Yana Pikman,Gerard M McGeehan,Kimberly Stegmaier,Anthony Letai,Eric S Fischer,X Shirley Liu,Scott A Armstrong

Diabetes 71:795-811 PubMed35043173

2022

Methazolamide Attenuates the Development of Diabetic Cardiomyopathy by Promoting β-Catenin Degradation in Type 1 Diabetic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Chen,Yilang Li,Xun Yuan,Wenchang Yuan,Conglin Li,Yue Zeng,Yuling Lian,Xiaoxia Qiu,Yuan Qin,Guiping Zhang,Xiawen Liu,Chengfeng Luo,Jian-Dong Luo,Ning Hou

Nature communications 13:183 PubMed35013300

2022

A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Yamanaka,Yuto Horiuchi,Saya Matsuoka,Kohki Kido,Kohei Nishino,Mayaka Maeno,Norio Shibata,Hidetaka Kosako,Tatsuya Sawasaki

Journal of medicinal chemistry 65:747-756 PubMed34965125

2021

Development of PDE6D and CK1α Degraders through Chemical Derivatization of FPFT-2216.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxing Teng,Wenchao Lu,Katherine A Donovan,Jialin Sun,Noah M Krupnick,Radosław P Nowak,Yen-Der Li,Adam S Sperling,Tinghu Zhang,Benjamin L Ebert,Eric S Fischer,Nathanael S Gray
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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