Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] - BSA and Azide free

• WB

Lab

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] - BSA and Azide free (AB242383)

This data was developed using ab108296, the same antibody clone in a different buffer formulation.

ab108296 was shown to react with CSNK1A1 in wild-type HCT116 cells in Western blot with loss of signal observed in a CSNK1A1 siRNA knockdown cell line. Cell lysates from wild-type HCT116 transfected with either scrambled siRNA or CSNK1A1 siRNA were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab108296 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (<a href='/en-us/products/primary-antibodies/csnk1a1-csnk1a1l-antibody-epr19612-ab108296'>ab108296</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT116 transfected with scrambled siRNA control lysate at 50 µg

Lane 2:

HCT116 transfected with siRNA specifically targeting CSNK1A1 cell lysate at 50 µg

false

• WB

Lab

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] - BSA and Azide free (AB242383)

Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108296).

All lanes:

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (<a href='/en-us/products/primary-antibodies/csnk1a1-csnk1a1l-antibody-epr19612-ab108296'>ab108296</a>) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 15 µg

Lane 2:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 39 kDa

false

• WB

Unknown

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] - BSA and Azide free (AB242383)

All lanes:

Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (<a href='/en-us/products/primary-antibodies/csnk1a1-csnk1a1l-antibody-epr19612-ab108296'>ab108296</a>) at 1/1000 dilution

Lane 1:

N-GST tagged Human CSNK1A1L full-length (aa1 to 337) recombinant protein 10ng

Lane 2:

N-GST tagged Human CSNK1A1 full-length (aa1 to 337) recombinant protein 10ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 39 kDa

false