Rabbit Recombinant Monoclonal CSNK2A1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (AB239873), expandable thumbnail](https://content.abcam.com/products/images/csnk2a1-antibody-ep1963y-bsa-and-azide-free-ab239873--western-blot-img147327.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (AB239873)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (AB239873), expandable thumbnail](https://content.abcam.com/products/images/csnk2a1-antibody-ep1963y-bsa-and-azide-free-ab239873--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140094.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (AB239873)")
![Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (AB239873), expandable thumbnail](https://content.abcam.com/products/images/csnk2a1-antibody-ep1963y-bsa-and-azide-free-ab239873--western-blot-img438543.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (AB239873)")
![Flow Cytometry (Intracellular) - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (AB239873), expandable thumbnail](https://content.abcam.com/products/images/csnk2a1-antibody-ep1963y-bsa-and-azide-free-ab239873--flow-cytometry-intracellular-img410092.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (AB239873)")
![Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (AB239873), expandable thumbnail](https://content.abcam.com/products/images/csnk2a1-antibody-ep1963y-bsa-and-azide-free-ab239873--western-blot-img410091.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (AB239873)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended |
| Mouse | Not recommended | Tested | Expected | Not recommended |
| Rat | Not recommended | Tested | Expected | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
8 products for Alternative Version
Target data
Function
Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine (PubMed:11239457, PubMed:11704824, PubMed:16193064, PubMed:18411307, PubMed:18583988, PubMed:18678890, PubMed:19188443, PubMed:20545769, PubMed:20625391, PubMed:22017874, PubMed:22406621, PubMed:24962073, PubMed:30898438, PubMed:31439799). Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection (PubMed:12631575, PubMed:19387551, PubMed:19387552). May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response (PubMed:12631575, PubMed:19387551, PubMed:19387552). During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage (PubMed:11704824, PubMed:19188443). Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation (PubMed:11239457). Phosphorylates a number of DNA repair proteins in response to DNA damage, such as MDC1, MRE11, RAD9A, RAD51 and HTATSF1, promoting their recruitment to DNA damage sites (PubMed:18411307, PubMed:18583988, PubMed:18678890, PubMed:20545769, PubMed:21482717, PubMed:22325354, PubMed:26811421, PubMed:28512243, PubMed:30898438, PubMed:35597237). Can also negatively regulate apoptosis (PubMed:16193064, PubMed:22184066). Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3 (PubMed:16193064). Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8 (PubMed:16193064). Phosphorylates YY1, protecting YY1 from cleavage by CASP7 during apoptosis (PubMed:22184066). Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV (PubMed:12631575, PubMed:19387550, PubMed:19387551, PubMed:19387552, PubMed:23123191). Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, ATF4, SRF, MAX, JUN, FOS, MYC and MYB (PubMed:12631575, PubMed:19387550, PubMed:19387551, PubMed:19387552, PubMed:23123191). Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function (PubMed:19387550). Mediates sequential phosphorylation of FNIP1, promoting its gradual interaction with Hsp90, leading to activate both kinase and non-kinase client proteins of Hsp90 (PubMed:30699359). Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1 (PubMed:19387549). Acts as an ectokinase that phosphorylates several extracellular proteins (PubMed:12631575, PubMed:19387550, PubMed:19387551, PubMed:19387552). During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV (PubMed:12631575, PubMed:19387550, PubMed:19387551, PubMed:19387552). Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation (PubMed:20625391, PubMed:22406621). Plays an important role in the circadian clock function by phosphorylating BMAL1 at 'Ser-90' which is pivotal for its interaction with CLOCK and which controls CLOCK nuclear entry (By similarity). Phosphorylates CCAR2 at 'Thr-454' in gastric carcinoma tissue (PubMed:24962073). Phosphorylates FMR1, promoting FMR1-dependent formation of a membraneless compartment (PubMed:30765518, PubMed:31439799). May phosphorylate histone H2A on 'Ser-1' (PubMed:38334665).
Alternative names
CK2A1, CSNK2A1, Casein kinase II subunit alpha, CK II alpha
Recommended products
Rabbit Recombinant Monoclonal CSNK2A1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1963Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239873 is the carrier-free version of Anti-CSNK2A1 antibody [EP1963Y] ab76040.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873), expandable thumbnail](https://content.abcam.com/products/images/csnk2a1-antibody-ep1963y-bsa-and-azide-free-ab239873--western-blot-img147327.jpg "Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873)")
Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CSNK2A1 antibody [EP1963Y] ab76040).
Lanes 1-2: Merged signal (red and green). Green - Anti-CSNK2A1 antibody [EP1963Y] ab76040 observed at 45 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-CSNK2A1 antibody [EP1963Y] ab76040 Anti-CSNK2A1 antibody [EP1963Y] was shown to specifically react with CSNK2A1 in wild-type HCT116 cells. Loss of signal was observed when knockout cell line Human CSNK2A1 knockout HCT116 cell line ab266864 (knockout cell lysate Human CSNK2A1 knockout HCT116 cell lysate ab257401) was used. Wild-type and CSNK2A1 knockout samples were subjected to SDS-PAGE. Anti-CSNK2A1 antibody [EP1963Y] ab76040 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CSNK2A1 antibody [EP1963Y] (Anti-CSNK2A1 antibody [EP1963Y] ab76040) at 1/1000 dilution
Lane 1: Wild-type HCT116 cell lysate at 20 µg
Lane 2: CSNK2A1 knockout HCT116 cell lysate at 20 µg
Lane 2: Western blot - Human CSNK2A1 knockout HCT116 cell line (Human CSNK2A1 knockout HCT116 cell line ab266864)
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873), expandable thumbnail](https://content.abcam.com/products/images/csnk2a1-antibody-ep1963y-bsa-and-azide-free-ab239873--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140094.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873)
Immunohistochemical analysis of CSNK2A1 in paraffin embedded human breast carcinoma tissue using Anti-CSNK2A1 antibody [EP1963Y] ab76040 at a 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CSNK2A1 antibody [EP1963Y] ab76040).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873), expandable thumbnail](https://content.abcam.com/products/images/csnk2a1-antibody-ep1963y-bsa-and-azide-free-ab239873--western-blot-img438543.jpg "Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873)")
Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873)
This data was developed using Anti-CSNK2A1 antibody [EP1963Y] ab76040, the same antibody clone in a different buffer formulation.
Anti-CSNK2A1 antibody [EP1963Y] ab76040 was shown to react with CSNK2A1 in wild-type HAP1 cells in Western blot with loss of signal observed in a CSNK2A1 knockout cell line. Wild-type HAP1 and CSNK2A1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-CSNK2A1 antibody [EP1963Y] ab76040 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.All lanes: Western blot - Anti-CSNK2A1 antibody [EP1963Y] (Anti-CSNK2A1 antibody [EP1963Y] ab76040) at 1/500 dilution
Lane 1: Wild-type HAP1 lysate at 30 µg
Lane 2: CSNK2A1 knock-out HAP1 lysate at 30 µg
![Flow Cytometry (Intracellular) - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873), expandable thumbnail](https://content.abcam.com/products/images/csnk2a1-antibody-ep1963y-bsa-and-azide-free-ab239873--flow-cytometry-intracellular-img410092.jpg "Flow Cytometry (Intracellular) - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873)")
Flow Cytometry (Intracellular) - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CSNK2A1 antibody [EP1963Y] ab76040).
Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CSNK2A1 with purified Anti-CSNK2A1 antibody [EP1963Y] ab76040 at 1:20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.![Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873), expandable thumbnail](https://content.abcam.com/products/images/csnk2a1-antibody-ep1963y-bsa-and-azide-free-ab239873--western-blot-img410091.jpg "Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873)")
Western blot - Anti-CSNK2A1 antibody [EP1963Y] - BSA and Azide free (ab239873)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CSNK2A1 antibody [EP1963Y] ab76040).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.All lanes: Western blot - Anti-CSNK2A1 antibody [EP1963Y] (Anti-CSNK2A1 antibody [EP1963Y] ab76040) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: Mouse brain lysate at 15 µg
Lane 3: Rat brian lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
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