AB10474

Anti-CSNK2A2 antibody

Name: Anti-CSNK2A2 antibody (ab10474) | Abcam
Brand: Abcam Limited
SKU: AB10474
Price: 545 USD
Availability: InStock
Rating: 5 (6 reviews)

(6 Reviews)

(8 Publications)

Rabbit Polyclonal CSNK2A2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CSNK2A2 aa 300 to C-terminus.

View Alternative Names

CK2A2, CSNK2A2, Casein kinase II subunit alpha', CK II alpha'

4 Images

Supplier Data

Immunoprecipitation - Anti-CSNK2A2 antibody (AB10474)

CSNK2A2 was immunoprecipitated from 1mg of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab10474 at 6 μg/ml. Western blot was performed from the immunoprecipitate using ab10474 at 1 μg/ml.

Lane 1 : ab10474 IP in HeLa whole cell lysate.

Lane 2 : Ctrl IgG instead of ab10474.

All lanes:

Immunoprecipitation - Anti-CSNK2A2 antibody (ab10474)

Predicted band size: 41 kDa

false

Exposure time: 10s

Unknown

Western blot - Anti-CSNK2A2 antibody (AB10474)

Detection of human and mouse CSNK2A1 by western blot.
Samples : Whole cell lysate (50 μg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer.
Antibody : Affinity purified rabbit anti-CSNK2A1 antibody A300-199A (lot A300-199A-5) used for WB at 0.1 μg/ml.
Detection : Chemiluminescence

All lanes:

Western blot - Anti-CSNK2A2 antibody (ab10474)

Predicted band size: 41 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-CSNK2A2 antibody (AB10474)

Detection of human CSNK2A2 by Western blot and immunoprecipitation.

A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v).

B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2 prime, or vimentin (v) siRNA.

C. RIPA extract (0.5mg) from HeLa cells.

Antibody : ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 mg/0.5 mg lysate (C) for IP.

Detection of human CSNK2A2 by Western blot and immunoprecipitation. A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v). B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2, or vimentin (v) siRNA. C. RIPA extract (0.5mg) from HeLa cells. Antibody : ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 ug/0.5 mg lysate (C) for IP.

All lanes:

Western blot - Anti-CSNK2A2 antibody (ab10474)

Predicted band size: 41 kDa

false

AbReview25167****

Western blot - Anti-CSNK2A2 antibody (AB10474)

All lanes:

Western blot - Anti-CSNK2A2 antibody (ab10474) at 1/500 dilution

Lane 1:

Whole cell lysate prepared from alpha prime knock out MEFs at 40 µg

Lane 2:

Whole cell lysate prepared from alpha prime heterozygous MEFs at 40 µg

Secondary

All lanes:

Goat anti rabbit IgG-HRP at 1/5000 dilution

Predicted band size: 41 kDa

Observed band size: 37 kDa

true

Exposure time: 30min

Image courtesy of an anonymous Abreview.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human CSNK2A2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P19784

Specificity

Reacts with the alpha prime subunit.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine (PubMed : 11239457, PubMed : 11704824, PubMed : 16193064, PubMed : 30898438). Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection (PubMed : 11704824, PubMed : 16193064, PubMed : 30898438). May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation (PubMed : 11239457). Phosphorylates a number of DNA repair proteins in response to DNA damage, such as MDC1, RAD9A, RAD51 and HTATSF1, promoting their recruitment to DNA damage sites (PubMed : 20545769, PubMed : 21482717, PubMed : 22325354, PubMed : 26811421, PubMed : 30898438, PubMed : 35597237). Can also negatively regulate apoptosis (PubMed : 19387551, PubMed : 19387552). Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3 (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8 (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function (PubMed : 19387550). Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1 (PubMed : 19387549). Acts as an ectokinase that phosphorylates several extracellular proteins (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). May phosphorylate histone H2A on 'Ser-1' (PubMed : 38334665).

See full target information CSNK2A2

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 24:100952 PubMed40113211

2025

Proteomics and Machine Learning-Based Approach to Decipher Subcellular Proteome of Mouse Heart.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyun Fang,Alin Rai,Seyed Sadegh Eslami,Kevin Huynh,Hsiao-Chi Liao,Agus Salim,David W Greening

Journal of extracellular biology 2:e125 PubMed38938901

2024

Cardiomyocyte intercellular signalling increases oxidative stress and reprograms the global- and phospho-proteome of cardiac fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Bethany Claridge,Alin Rai,Jarmon G Lees,Haoyun Fang,Shiang Y Lim,David W Greening

Autophagy 20:994-1014 PubMed37938186

2023

CSNK2 suppresses autophagy by activating FLN-NHL-containing TRIM proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Helene Hoenigsperger,Lennart Koepke,Dhiraj Acharya,Victoria Hunszinger,Dennis Freisem,Alexander Grenzner,Sebastian Wiese,Frank Kirchhoff,Michaela U Gack,Konstantin M J Sparrer

Journal of biological rhythms 34:144-153 PubMed30898060

2019

Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew D Beale,Emily Kruchek,Stephen J Kitcatt,Erin A Henslee,Jack S W Parry,Gabriella Braun,Rita Jabr,Malcolm von Schantz,John S O'Neill,Fatima H Labeed

Cell death discovery 4:39 PubMed29531836

2018

Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells.

Applications

Species

Unspecified reactive species

Ashfaqul Hoque,Priyadharshini Sivakumaran,Simon T Bond,Naomi X Y Ling,Anne M Kong,John W Scott,Nadeeka Bandara,Damián Hernández,Guei-Sheung Liu,Raymond C B Wong,Michael T Ryan,Derek J Hausenloy,Bruce E Kemp,Jonathan S Oakhill,Brian G Drew,Alice Pébay,Shiang Y Lim

Molecular and cellular biology 33:3461-72 PubMed23816881

2013

The casein kinase 2-nrf1 axis controls the clearance of ubiquitinated proteins by regulating proteasome gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiki Tsuchiya,Hiroaki Taniguchi,Yoshiyuki Ito,Tomoko Morita,M Rezaul Karim,Norihito Ohtake,Kousuke Fukagai,Takao Ito,Shota Okamuro,Shun-Ichiro Iemura,Tohru Natsume,Eisuke Nishida,Akira Kobayashi

Molecular and cellular biochemistry 353:81-91 PubMed21431367

2011

The cytosolic protein kinase CK2 phosphorylates cardiac calsequestrin in intact cells.

Applications

Species

Human

Timothy P McFarland,Naama H Sleiman,Daniel B Yaeger,Steven E Cala

The Journal of biological chemistry 285:1661-70 PubMed19933278

2009

Differential regulation of mitogen- and stress-activated protein kinase-1 and -2 (MSK1 and MSK2) by CK2 following UV radiation.

Applications

Species

Unspecified reactive species

Kellie A Jacks,C Anne Koch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CSNK2A2 antibody

Immunoprecipitation - Anti-CSNK2A2 antibody (AB10474)

Western blot - Anti-CSNK2A2 antibody (AB10474)

Western blot - Anti-CSNK2A2 antibody (AB10474)

Western blot - Anti-CSNK2A2 antibody (AB10474)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

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