Rabbit Polyclonal CSNK2A2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CSNK2A2 aa 300 to C-terminus.
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Publications
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- Journal of biological rhythms 34:144-1532019Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew D Beale et. al.PubMed 30898060
- Cell death discovery 4:392018Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells.
- Molecular and cellular biology 33:3461-722013The casein kinase 2-nrf1 axis controls the clearance of ubiquitinated proteins by regulating proteasome gene expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshiki Tsuchiya et. al.PubMed 23816881
- Molecular and cellular biochemistry 353:81-912011The cytosolic protein kinase CK2 phosphorylates cardiac calsequestrin in intact cells.
- The Journal of biological chemistry 285:1661-702009Differential regulation of mitogen- and stress-activated protein kinase-1 and -2 (MSK1 and MSK2) by CK2 following UV radiation.Applications:WBReactive species:Unspecified reactive speciesKellie A Jacks,C Anne KochPubMed 19933278
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CSNK2A2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P19784
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Bat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Elephant | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant | Dilution info - | Notes - |
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Target data
Function
Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine (PubMed:11239457, PubMed:11704824, PubMed:16193064, PubMed:30898438). Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection (PubMed:11704824, PubMed:16193064, PubMed:30898438). May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response (PubMed:12631575, PubMed:19387551, PubMed:19387552). During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage (PubMed:12631575, PubMed:19387551, PubMed:19387552). Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation (PubMed:11239457). Phosphorylates a number of DNA repair proteins in response to DNA damage, such as MDC1, RAD9A, RAD51 and HTATSF1, promoting their recruitment to DNA damage sites (PubMed:20545769, PubMed:21482717, PubMed:22325354, PubMed:26811421, PubMed:30898438, PubMed:35597237). Can also negatively regulate apoptosis (PubMed:19387551, PubMed:19387552). Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3 (PubMed:12631575, PubMed:19387551, PubMed:19387552). Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8 (PubMed:12631575, PubMed:19387551, PubMed:19387552). Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV (PubMed:12631575, PubMed:19387551, PubMed:19387552). Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB (PubMed:12631575, PubMed:19387551, PubMed:19387552). Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function (PubMed:19387550). Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1 (PubMed:19387549). Acts as an ectokinase that phosphorylates several extracellular proteins (PubMed:12631575, PubMed:19387551, PubMed:19387552). During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV (PubMed:12631575, PubMed:19387551, PubMed:19387552). May phosphorylate histone H2A on 'Ser-1' (PubMed:38334665).
Alternative names
CK2A2, CSNK2A2, Casein kinase II subunit alpha', CK II alpha'
Recommended products
Rabbit Polyclonal CSNK2A2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CSNK2A2 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CSNK2A2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P19784
- Purification technique
- Affinity purification Immunogen
- Specificity
Reacts with the alpha prime subunit.
- Concentration
- Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis.
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Expected
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Predicted
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4 product images
Immunoprecipitation - Anti-CSNK2A2 antibody (ab10474)
CSNK2A2 was immunoprecipitated from 1mg of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab10474 at 6 μg/ml. Western blot was performed from the immunoprecipitate using ab10474 at 1 μg/ml.
Lane 1: ab10474 IP in HeLa whole cell lysate.
Lane 2: Ctrl IgG instead of ab10474.
All lanes: Immunoprecipitation - Anti-CSNK2A2 antibody (ab10474)
Predicted band size: 41 kDa
Exposure time: 10s
Western blot - Anti-CSNK2A2 antibody (ab10474)
Detection of human and mouse CSNK2A1 by western blot.
Samples: Whole cell lysate (50 μg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer.
Antibody: Affinity purified rabbit anti-CSNK2A1 antibody A300-199A (lot A300-199A-5) used for WB at 0.1 μg/ml.
Detection: ChemiluminescenceAll lanes: Western blot - Anti-CSNK2A2 antibody (ab10474)
Predicted band size: 41 kDa
Exposure time: 30s
Image courtesy of an anonymous customer review.Western blot - Anti-CSNK2A2 antibody (ab10474)
All lanes: Western blot - Anti-CSNK2A2 antibody (ab10474) at 1/500 dilution
Lane 1: Whole cell lysate prepared from alpha prime knock out MEFs at 40 µg
Lane 2: Whole cell lysate prepared from alpha prime heterozygous MEFs at 40 µg
SecondaryAll lanes: Goat anti rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 37 kDa
Exposure time: 30min
Western blot - Anti-CSNK2A2 antibody (ab10474)
Detection of human CSNK2A2 by Western blot and immunoprecipitation.
A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v).
B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2 prime, or vimentin (v) siRNA.
C. RIPA extract (0.5mg) from HeLa cells.
Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 mg/0.5 mg lysate (C) for IP.
Detection of human CSNK2A2 by Western blot and immunoprecipitation. A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v). B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2, or vimentin (v) siRNA. C. RIPA extract (0.5mg) from HeLa cells. Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 ug/0.5 mg lysate (C) for IP.
All lanes: Western blot - Anti-CSNK2A2 antibody (ab10474)
Predicted band size: 41 kDa
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