Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Flow cytometric analysis of living A-375 (Human malignant melanoma cell line) cells, using anti-CTAG1B antibody (Red), compared to an isotype control (green) at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Paraffin embedded human testis tissue stained for CTAG1B using a mouse monoclonal [OTI4D8] to CTAG1B at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer, pH9.0, 120°C for 3mins.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Flow cytometric analysis of living NCI-H1299 (Human lung carcinoma cell line) cells, using anti-CTAG1B antibody (Red), compared to an isotype control (green) at 1/100 dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Flow cytometric analysis of living HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with CTAG1B overexpression plasmid (Red) and empty vector (Blue) using anti-CTAG1B antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Paraffin embedded human pancreas tissue stained for CTAG1B using a mouse monoclonal [OTI4D8] to CTAG1B at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3mins.

• WB

Supplier Data

Western blot - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

All lanes:

Mouse monoclonal [OTI4D8] to CTAG1B at 1/500 dilution

Lane 1:

NCI-H1299 (Human lung carcinoma cell line) cell extracts at 35 µg

Lane 2:

A-375 (Human malignant melanoma cell line) cell extracts at 35 µg

Predicted band size: 18 kDa

false

• ICC

Supplier Data

Immunocytochemistry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY CTAG1B stained for CTAG1B (green) using a mouse monoclonal [OTI4D8] to CTAG1B at 1/100 dilution in ICC.

• WB

Supplier Data

Western blot - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Transfected for 48 hrs then lysed.

All lanes:

Mouse monoclonal [OTI4D8] to CTAG1B at 1/2000 dilution

Lane 1:

HEK-293T cells transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cells were transfected with pCMV6-ENTRY CTAG1B cDNA at 5 µg

Predicted band size: 18 kDa

false