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Mouse Monoclonal CTAG1B antibody. Suitable for IHC-P, ICC, Flow Cyt, WB and reacts with Human, Transfected cell line, Transfected cell lysate - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CTAG1A.

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Images

Immunocytochemistry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373), expandable thumbnail
  • Western blot - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373), expandable thumbnail
  • Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373), expandable thumbnail
  • Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (AB275373), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3
Constituents: 92% PBS, 8% Trehalose

Form
Lyophilized
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human CTAG1A. Database link P78358

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCFlow CytWB
Human
Tested
Expected
Tested
Tested
Transfected cell line
Not recommended
Tested
Not recommended
Not recommended
Transfected cell lysate - Human
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/150
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell line, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Not recommended
Not recommended

Species
Transfected cell line
Dilution info
-
Notes

-

Target data

Alternative names

CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B, CTAG1A, Cancer/testis antigen 1, Autoimmunogenic cancer/testis antigen NY-ESO-1, Cancer/testis antigen 6.1, L antigen family member 2, CT6.1, LAGE-2

Recommended products

Mouse Monoclonal CTAG1B antibody. Suitable for IHC-P, ICC, Flow Cyt, WB and reacts with Human, Transfected cell line, Transfected cell lysate - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CTAG1A.

Key facts

Isotype
IgG1
Form
Lyophilized
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human CTAG1A. Database link P78358
Clone number
OTI4D8
Purification technique
Affinity purification Protein A/G
Reconstitution
For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CTAG1B also known as NY-ESO-1 is a cancer/testis antigen with an approximate molecular mass of 22 kDa. This protein is absent in most normal tissues but shows expression in the testes and a variety of tumors. CTAG1B is recognized for its potential role in immune targeting due to its restricted expression profile. The protein plays a part in antigenic presentation and stimulates T-cell response making it a significant focus for cancer immunotherapy research.

Biological function summary

CTAG1B is involved in the immune response against tumors. It is part of the group called cancer/testis antigens due to its low expression in normal tissues except the testes. This restricted pattern allows CTAG1B to function in distinguishing between tumor and normal cells which is important for targeted therapies. It does not belong to a specific multiprotein complex but contributes to immune recognition by presenting as an antigenic determinant.

Pathways

CTAG1B engages predominantly in cellular processes associated with the immune system's adaptive arm. It plays a role in the antigen processing and presentation pathway where it functions to engage CD8+ T cells. The protein associates with other antigens expressed in cancer such as MAGE-A1 in enabling an immune response against tumor cells. By acting alongside these proteins CTAG1B supports pathways leading to the recognition and destruction of malignant cells.

Associated diseases and disorders

CTAG1B shows a strong connection to cancer particularly in melanoma and ovarian cancer. It is considered a biomarker for these cancers due to its restricted expression and detection in tumor tissues. In cancer CTAG1B interacts with immune checkpoint proteins such as PD-L1 where it helps sustain and enhance immune responses. This relationship has made CTAG1B a promising target in immunotherapy aiming to improve cancer treatment outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373), expandable thumbnail

    Immunocytochemistry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY CTAG1B stained for CTAG1B (green) using a mouse monoclonal [OTI4D8] to CTAG1B at 1/100 dilution in ICC.

  • Western blot - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373), expandable thumbnail

    Western blot - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Transfected for 48 hrs then lysed.

    All lanes: Mouse monoclonal [OTI4D8] to CTAG1B at 1/2000 dilution

    Lane 1: HEK-293T cells transfected with pCMV6-ENTRY control cDNA at 5 µg

    Lane 2: HEK-293T cells were transfected with pCMV6-ENTRY CTAG1B cDNA at 5 µg

    Predicted band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Paraffin embedded human pancreas tissue stained for CTAG1B using a mouse monoclonal [OTI4D8] to CTAG1B at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3mins.

  • Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373), expandable thumbnail

    Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Flow cytometric analysis of living HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with CTAG1B overexpression plasmid (Red) and empty vector (Blue) using anti-CTAG1B antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control at 1/100 dilution.

  • Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373), expandable thumbnail

    Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Flow cytometric analysis of living NCI-H1299 (Human lung carcinoma cell line) cells, using anti-CTAG1B antibody (Red), compared to an isotype control (green) at 1/100 dilution.

  • Western blot - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373), expandable thumbnail

    Western blot - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    All lanes: Mouse monoclonal [OTI4D8] to CTAG1B at 1/500 dilution

    Lane 1: NCI-H1299 (Human lung carcinoma cell line) cell extracts at 35 µg

    Lane 2: A-375 (Human malignant melanoma cell line) cell extracts at 35 µg

    Predicted band size: 18 kDa

  • Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373), expandable thumbnail

    Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Flow cytometric analysis of living A-375 (Human malignant melanoma cell line) cells, using anti-CTAG1B antibody (Red), compared to an isotype control (green) at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Paraffin embedded human testis tissue stained for CTAG1B using a mouse monoclonal [OTI4D8] to CTAG1B at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer, pH9.0, 120°C for 3mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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