Anti-CTAG1B antibody [SP349] - BSA and Azide free
- RabMAb
- Recombinant
- What is this?
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(1 Publication)
Rabbit Recombinant Monoclonal CTAG1B antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication.
View Alternative Names
CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B, CTAG1A, Cancer/testis antigen 1, Autoimmunogenic cancer/testis antigen NY-ESO-1, Cancer/testis antigen 6.1, L antigen family member 2, CT6.1, LAGE-2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (AB242416)
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CTAG1B with ab223498 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223498)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (AB242416)
Formalin-fixed, paraffin-embedded human testis tissue stained for CTAG1B using ab223498 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223498)
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-CTAG1B antibody [SP349] - BSA and Azide free (AB242416)
Flow cytometric analysis of ab223498 staining HeLa (human epithelial cell line from cervix adenocarcinoma) cells for CTAG1B (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223498)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (AB242416)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling CTAG1B with Purified ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223498)
- Flow Cyt
Unknown
Flow Cytometry - Anti-CTAG1B antibody [SP349] - BSA and Azide free (AB242416)
Flow Cytometry analysis of NCI-H1299 (human lung carcinoma epithelial cell) cells labeling CTAG1B with purified ab223498 at 1 : 380 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242416)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (AB242416)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CTAG1B with Purified ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223498)
Related conjugates and formulations (1)
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Anti-CTAG1B antibody [SP349]
Reactivity data
Product details
ab242416 is the carrier-free version of ab223498.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CTAG1B is involved in the immune response against tumors. It is part of the group called cancer/testis antigens due to its low expression in normal tissues except the testes. This restricted pattern allows CTAG1B to function in distinguishing between tumor and normal cells which is important for targeted therapies. It does not belong to a specific multiprotein complex but contributes to immune recognition by presenting as an antigenic determinant.
Pathways
CTAG1B engages predominantly in cellular processes associated with the immune system's adaptive arm. It plays a role in the antigen processing and presentation pathway where it functions to engage CD8+ T cells. The protein associates with other antigens expressed in cancer such as MAGE-A1 in enabling an immune response against tumor cells. By acting alongside these proteins CTAG1B supports pathways leading to the recognition and destruction of malignant cells.
Product protocols
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Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Cell reports. Medicine 5:101572 PubMed38754420
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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