Skip to main content

Rabbit Recombinant Monoclonal CTAG1B antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Flow Cytometry - Anti-CTAG1B antibody [SP349] - BSA and Azide free (AB242416), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (AB242416), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (AB242416), expandable thumbnail
  • Flow Cytometry - Anti-CTAG1B antibody [SP349] - BSA and Azide free (AB242416), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (AB242416), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-P
Human
Tested
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Alternative names

CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B, CTAG1A, Cancer/testis antigen 1, Autoimmunogenic cancer/testis antigen NY-ESO-1, Cancer/testis antigen 6.1, L antigen family member 2, CT6.1, LAGE-2

Recommended products

Rabbit Recombinant Monoclonal CTAG1B antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
SP349
Purification technique
Affinity purification Protein A/G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab242416 is the carrier-free version of Anti-CTAG1B antibody [SP349] ab223498.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CTAG1B also known as NY-ESO-1 is a cancer/testis antigen with an approximate molecular mass of 22 kDa. This protein is absent in most normal tissues but shows expression in the testes and a variety of tumors. CTAG1B is recognized for its potential role in immune targeting due to its restricted expression profile. The protein plays a part in antigenic presentation and stimulates T-cell response making it a significant focus for cancer immunotherapy research.

Biological function summary

CTAG1B is involved in the immune response against tumors. It is part of the group called cancer/testis antigens due to its low expression in normal tissues except the testes. This restricted pattern allows CTAG1B to function in distinguishing between tumor and normal cells which is important for targeted therapies. It does not belong to a specific multiprotein complex but contributes to immune recognition by presenting as an antigenic determinant.

Pathways

CTAG1B engages predominantly in cellular processes associated with the immune system's adaptive arm. It plays a role in the antigen processing and presentation pathway where it functions to engage CD8+ T cells. The protein associates with other antigens expressed in cancer such as MAGE-A1 in enabling an immune response against tumor cells. By acting alongside these proteins CTAG1B supports pathways leading to the recognition and destruction of malignant cells.

Associated diseases and disorders

CTAG1B shows a strong connection to cancer particularly in melanoma and ovarian cancer. It is considered a biomarker for these cancers due to its restricted expression and detection in tumor tissues. In cancer CTAG1B interacts with immune checkpoint proteins such as PD-L1 where it helps sustain and enhance immune responses. This relationship has made CTAG1B a promising target in immunotherapy aiming to improve cancer treatment outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416), expandable thumbnail

    Flow Cytometry - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416)

    Flow Cytometry analysis of NCI-H1299 (human lung carcinoma epithelial cell) cells labeling CTAG1B with purified Anti-CTAG1B antibody [SP349] ab223498 at 1:380 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242416)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CTAG1B with Purified Anti-CTAG1B antibody [SP349] ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTAG1B antibody [SP349] ab223498)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling CTAG1B with Purified Anti-CTAG1B antibody [SP349] ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTAG1B antibody [SP349] ab223498)

  • Flow Cytometry - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416), expandable thumbnail

    Flow Cytometry - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416)

    Flow cytometric analysis of Anti-CTAG1B antibody [SP349] ab223498 staining HeLa (human epithelial cell line from cervix adenocarcinoma) cells for CTAG1B (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTAG1B antibody [SP349] ab223498)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416)

    Formalin-fixed, paraffin-embedded human testis tissue stained for CTAG1B using Anti-CTAG1B antibody [SP349] ab223498 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTAG1B antibody [SP349] ab223498)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] - BSA and Azide free (ab242416)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CTAG1B with Anti-CTAG1B antibody [SP349] ab223498 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTAG1B antibody [SP349] ab223498)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com