AB129181

Anti-CtBP1 antibody [EPR6800]

RabMAb
Recombinant
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(15 Publications)

Rabbit Recombinant Monoclonal CTBP1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications.

View Alternative Names

CTBP, CTBP1, C-terminal-binding protein 1, CtBP1

5 Images

Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] (AB129181)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] (AB129181)

Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling CtBP1 (red) with ab129181 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] (AB129181)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] (AB129181)

ab129181 at 1/100 dilution staining CtBP1 in formalin-fixed paraffin-embedded human colon tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] (AB129181)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] (AB129181)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CtBP1 with ab129181 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control : PBS only.
Nuclear counter stain : DAPI.

Unknown

Western blot - Anti-CtBP1 antibody [EPR6800] (AB129181)

All lanes:

Western blot - Anti-CtBP1 antibody [EPR6800] (ab129181) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 47 kDa

Observed band size: 48 kDa

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OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] (AB129181)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] (AB129181)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-CtBP1 antibody [EPR6800] - BSA and Azide free
HRP

HRP Anti-CtBP1 antibody [EPR6800]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6800

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2000", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.

See full target information CTBP1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:108 PubMed40001144

2025

CXXC5 function blockade promotes diabetic wound healing through stimulating fibroblast and vascular endothelial cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yutong Chen,Xiaofeng Ding,Zhouji Ma,Shuai Shao,Heyan Huang,Yumeng Huang,Beizhi Wang,Hao Zhang,Qian Tan

Communications biology 7:1174 PubMed39294274

2024

CTBP1 links metabolic syndrome to polycystic ovary syndrome through interruption of aromatase and SREBP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Kong,Guang Yang,Xu Feng,Zhaodong Ji,Xiaoling Wang,Yang Shao,Jiao Meng,Guidong Yao,Chunxia Ren,Gong Yang

Biomedicines 10: PubMed36551887

2022

Angiotensin II Inhibits Adipogenic Differentiation and Promotes Mature Adipocyte Browning through the Corepressor CtBP1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuying Liang,Jingwen Sun,Haijing Guan,Qingyu Zhu,Wenjuan Yao

Biomedicines 9: PubMed34944635

2021

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuying Liang,Haijing Guan,Jingwen Sun,Yan Qi,Wenjuan Yao

Oncology letters 22:810 PubMed34630717

2021

CTBP1 strengthens the cisplatin resistance of gastric cancer cells by upregulating RAD51 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yuluo Wu,Haiyang Zhao

Journal of cellular and molecular medicine 24:11445-11456 PubMed32910558

2020

CtBP1 promotes tumour-associated macrophage infiltration and progression in non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenxing Wang,Yan Zhao,Hongyan Xu,Feihai Liang,Qingxu Zou,Chen Wang,Jingyuan Jiang,Fengwu Lin

Journal of cellular physiology 236:427-439 PubMed32583425

2020

LINC01426 contributes to clear cell renal cell carcinoma progression by modulating CTBP1/miR-423-5p/FOXM1 axis via interacting with IGF2BP1.

Applications

Unspecified application

Species

Unspecified reactive species

YuFeng Jiang,HaiMin Zhang,Wei Li,Yang Yan,XuDong Yao,WenYu Gu

Molecular carcinogenesis 59:512-519 PubMed32124501

2020

CtBP1 transactivates RAD51 and confers cisplatin resistance to breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Deng,Wanjun Guo,Ning Xu,Fulun Li,Jian Li

Journal of breast cancer 23:20-35 PubMed32140267

2020

Protocatechuic Aldehyde Represses Proliferation and Migration of Breast Cancer Cells through Targeting C-terminal Binding Protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Deng,Wanjun Guo,Guancheng Li,Shuang Li,Hong Li,Xinyan Li,Bei Niu,Mingzhu Song,Yamei Zhang,Zhijian Xu,Fulun Li

International journal of biological sciences 15:1460-1471 PubMed31337976

2019

The CtBP1-HDAC1/2-IRF1 transcriptional complex represses the expression of the long noncoding RNA GAS5 in human osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinliang Zhang,Kaili Du,Zhenkai Lou,Keyuan Ding,Fan Zhang,Jinwen Zhu,Zhen Chang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CtBP1 antibody [EPR6800]

Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] (AB129181)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] (AB129181)

Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] (AB129181)

Western blot - Anti-CtBP1 antibody [EPR6800] (AB129181)

OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] (AB129181)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (15)

Product promise