Rabbit Recombinant Monoclonal CTBP1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications.
Images
![Western blot - Anti-CtBP1 antibody [EPR6800] (AB129181), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-ab129181--western-blot-img116054.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CtBP1 antibody [EPR6800] (AB129181)")
![Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] (AB129181), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-ab129181--immunocytochemistry-immunofluorescence-img65889.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] (AB129181)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] (AB129181), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-ab129181--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73485.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] (AB129181)")
![Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] (AB129181), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-ab129181--flow-cytometry-intracellular-img26384.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] (AB129181)")
![OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] (AB129181), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-ab129181--oi-rd-scanning-img97921.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] (AB129181)")
Publications
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- Biomedicines 10:2022Angiotensin II Inhibits Adipogenic Differentiation and Promotes Mature Adipocyte Browning through the Corepressor CtBP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiuying Liang,Jingwen Sun,Haijing Guan,Qingyu Zhu,Wenjuan YaoPubMed 36551887
- Biomedicines 9:2021Comparative Proteomic Analysis of tPVAT during Ang II Infusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiuying Liang,Haijing Guan,Jingwen Sun,Yan Qi,Wenjuan YaoPubMed 34944635
- Oncology letters 22:8102021CTBP1 strengthens the cisplatin resistance of gastric cancer cells by upregulating RAD51 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuluo Wu,Haiyang ZhaoPubMed 34630717
- Journal of cellular and molecular medicine 24:11445-114562020CtBP1 promotes tumour-associated macrophage infiltration and progression in non-small-cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenxing Wang et. al.PubMed 32910558
- Journal of cellular physiology 236:427-4392020LINC01426 contributes to clear cell renal cell carcinoma progression by modulating CTBP1/miR-423-5p/FOXM1 axis via interacting with IGF2BP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuFeng Jiang,HaiMin Zhang,Wei Li,Yang Yan,XuDong Yao,WenYu GuPubMed 32583425
- Molecular carcinogenesis 59:512-5192020CtBP1 transactivates RAD51 and confers cisplatin resistance to breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Deng,Wanjun Guo,Ning Xu,Fulun Li,Jian LiPubMed 32124501
- Journal of breast cancer 23:20-352020Protocatechuic Aldehyde Represses Proliferation and Migration of Breast Cancer Cells through Targeting C-terminal Binding Protein 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Deng et. al.PubMed 32140267
- International journal of biological sciences 15:1460-14712019The CtBP1-HDAC1/2-IRF1 transcriptional complex represses the expression of the long noncoding RNA GAS5 in human osteosarcoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinliang Zhang et. al.PubMed 31337976
- Oncology reports 42:67-782019CtBP1 interacts with SOX2 to promote the growth, migration and invasion of lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifei Liu et. al.PubMed 31059077
- Genes & development 30:1822-362016Lysine-specific demethylase 1 promotes brown adipose tissue thermogenesis via repressing glucocorticoid activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXing Zeng et. al.PubMed 27566776
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.
Alternative names
CTBP, CTBP1, C-terminal-binding protein 1, CtBP1
Recommended products
Rabbit Recombinant Monoclonal CTBP1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR6800
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.5 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-CtBP1 antibody [EPR6800] (ab129181), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-ab129181--western-blot-img116054.jpg "Western blot - Anti-CtBP1 antibody [EPR6800] (ab129181)")
Western blot - Anti-CtBP1 antibody [EPR6800] (ab129181)
All lanes: Western blot - Anti-CtBP1 antibody [EPR6800] (ab129181) at 1/1000 dilution
Lane 1: SH-SY5Y cell lysate at 10 µg
Lane 2: Raji cell lysate at 10 µg
Lane 3: A549 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 47 kDa
Observed band size: 48 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] (ab129181), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-ab129181--immunocytochemistry-immunofluorescence-img65889.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] (ab129181)")
Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] (ab129181)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CtBP1 with ab129181 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] (ab129181), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-ab129181--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73485.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] (ab129181)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] (ab129181)
ab129181, at 1/100 dilution, staining CtBP1 in formalin-fixed, paraffin-embedded human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] (ab129181), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-ab129181--flow-cytometry-intracellular-img26384.jpg "Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] (ab129181)")
Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] (ab129181)
Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling CtBP1 (red) with ab129181 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
![OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] (ab129181), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-ab129181--oi-rd-scanning-img97921.jpg "OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] (ab129181)")
OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] (ab129181)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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