Anti-CtBP1 antibody [EPR6800] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335)

This data was developed using ab129181, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling CtBP1 (red) with ab129181 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335)

This data was developed using ab129181, the same antibody clone in a different buffer formulation.

ab129181, at 1/100 dilution, staining CtBP1 in formalin-fixed, paraffin-embedded human colon tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335)

This data was developed using ab129181, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CtBP1 with ab129181 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control : PBS only.
Nuclear counter stain : DAPI.

• WB

Unknown

Western blot - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335)

This data was developed using ab129181, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CtBP1 antibody [EPR6800] (<a href='/en-us/products/primary-antibodies/ctbp1-antibody-epr6800-ab129181'>ab129181</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 47 kDa

Observed band size: 48 kDa

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• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.