Rabbit Recombinant Monoclonal CTBP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-bsa-and-azide-free-ab248335--western-blot-img116054.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335)")
![Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-bsa-and-azide-free-ab248335--immunocytochemistry-immunofluorescence-img65889.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-bsa-and-azide-free-ab248335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73485.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335)")
![Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-bsa-and-azide-free-ab248335--flow-cytometry-intracellular-img26384.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335)")
![OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-bsa-and-azide-free-ab248335--oi-rd-scanning-img97921.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (AB248335)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.
Alternative names
CTBP, CTBP1, C-terminal-binding protein 1, CtBP1
Recommended products
Rabbit Recombinant Monoclonal CTBP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6800
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.5 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248335 is the carrier-free version of Anti-CtBP1 antibody [EPR6800] ab129181.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-bsa-and-azide-free-ab248335--western-blot-img116054.jpg "Western blot - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)")
Western blot - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
This data was developed using Anti-CtBP1 antibody [EPR6800] ab129181, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CtBP1 antibody [EPR6800] (Anti-CtBP1 antibody [EPR6800] ab129181) at 1/1000 dilution
Lane 1: SH-SY5Y cell lysate at 10 µg
Lane 2: Raji cell lysate at 10 µg
Lane 3: A549 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 47 kDa
Observed band size: 48 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-bsa-and-azide-free-ab248335--immunocytochemistry-immunofluorescence-img65889.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)")
Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
This data was developed using Anti-CtBP1 antibody [EPR6800] ab129181, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CtBP1 with Anti-CtBP1 antibody [EPR6800] ab129181 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-bsa-and-azide-free-ab248335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73485.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
This data was developed using Anti-CtBP1 antibody [EPR6800] ab129181, the same antibody clone in a different buffer formulation.Anti-CtBP1 antibody [EPR6800] ab129181, at 1/100 dilution, staining CtBP1 in formalin-fixed, paraffin-embedded human colon tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-bsa-and-azide-free-ab248335--flow-cytometry-intracellular-img26384.jpg "Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)")
Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
This data was developed using Anti-CtBP1 antibody [EPR6800] ab129181, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling CtBP1 (red) with Anti-CtBP1 antibody [EPR6800] ab129181 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
![OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335), expandable thumbnail](https://content.abcam.com/products/images/ctbp1-antibody-epr6800-bsa-and-azide-free-ab248335--oi-rd-scanning-img97921.jpg "OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)")
OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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