Rabbit Polyclonal CTBP2 antibody. C-terminal. Suitable for IHC-P, ICC, WB, IHC-Fr and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human CTBP2 aa 400 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CTBP2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56545
Reactivity data
Select an application| IHC-P | ICC | WB | IHC-Fr | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Expected |
| Mouse | Tested | Expected | Expected | Tested |
| Rat | Tested | Expected | Tested | Tested |
| Cow | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.10000-0.50000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation (By similarity). Isoform 2 probably acts as a scaffold for specialized synapses.
Alternative names
C-terminal-binding protein 2, CtBP2, CTBP2
Recommended products
Rabbit Polyclonal CTBP2 antibody. C-terminal. Suitable for IHC-P, ICC, WB, IHC-Fr and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human CTBP2 aa 400 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CTBP2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56545
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CTBP2 also known as C-terminal binding protein 2 is a transcriptional co-repressor involved in regulating gene expression. It is a protein with a molecular mass of approximately 48 kDa. CTBP2 is expressed in various tissues with high levels in neural muscular and epithelial cells. Mechanically CTBP2 operates by interacting with transcription factors and histone-modifying enzymes modulating chromatin structure to control access to DNA.
- Biological function summary
CTBP2 plays a significant role in cellular processes such as development and differentiation. It frequently acts as part of multi-protein complexes interacting with diverse partners to facilitate transcriptional repression. These interactions are essential for balancing gene activity ensuring appropriate tissue development and function. CTBP2 influences cellular dynamics by regulating target genes implicated in growth survival and metabolic pathways.
- Pathways
CTBP2 integrates into significant signaling pathways like Wnt and TGF-beta where it reinforces regulatory control by interacting with critical factors such as beta-catenin or Smad proteins. It collaborates with other proteins like CTBP1 dictating cellular responses and influencing pathophysiological processes. By coordinating these pathways CTBP2 supports cellular homeostasis impacting tissues in response to external and internal cues.
- Associated diseases and disorders
CTBP2 has associations with cancer and neurodegenerative diseases. Its overexpression or altered activity links to cancer progression as it promotes a transcriptional environment conducive to tumor growth. Proteins such as E-cadherin connect with CTBP2 in metastasis exemplifying its role in cancer cell dissemination. Additionally changes in CTBP2 function relate to neurodegenerative conditions affecting nerve cell survival and synaptic activity pointing to its involvement in neuronal health and disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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7 product images
Western blot - Anti-CTBP2 antibody - C-terminal (ab190933)
All lanes: Western blot - Anti-CTBP2 antibody - C-terminal (ab190933) at 0.5 µg/mL
Lane 1: Rat brain tissue lysate
Lane 2: Rat spleen tissue lysate
Lane 3: HeLa cell lysate
Lane 4: 293T cell lysate
Lane 5: COLO320 cell lysate
Lane 6: U87 cell lysate
Lane 7: SW620 cell lysate
Predicted band size: 49 kDa
Immunohistochemistry (Frozen sections) - Anti-CTBP2 antibody - C-terminal (ab190933)
CTBP2 was detected in frozen section of mouse intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Immunocytochemistry - Anti-CTBP2 antibody - C-terminal (ab190933)
CTBP2 was detected in immunocytochemical section of human Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody - C-terminal (ab190933)
CTBP2 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody - C-terminal (ab190933)
CTBP2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Immunohistochemistry (Frozen sections) - Anti-CTBP2 antibody - C-terminal (ab190933)
CTBP2 was detected in frozen section of rat intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody - C-terminal (ab190933)
CTBP2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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