Anti-CTBP2 antibody [EPR7611(B)]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (AB128871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling CTBP2 with purified ab128871 at 1 : 6000 (0.021 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (AB128871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling CTBP2 with purified ab128871 at 1 : 6000 (0.021 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (AB128871)

Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling CTBP2 with purified ab128871 at 1 : 50 dilution (2.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CTBP2 antibody [EPR7611(B)] (AB128871)

Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling CTBP2 with purified ab128871 at 1/20 dilution (5 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (AB128871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling CTBP2 with purified ab128871 at 1 : 12000 (0.011 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (AB128871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling CTBP2 with Purified ab128871 at 1 : 12000 (0.011 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

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Western blot - Anti-CTBP2 antibody [EPR7611(B)] (AB128871)

All lanes:

Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Mouse brain lysate at 20 µg

Lane 4:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

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