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Rabbit Recombinant Monoclonal CTBP2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (AB248197), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (AB248197), expandable thumbnail
  • Western blot - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (AB248197), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (AB248197), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (AB248197), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Rat
Tested
Expected
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Version

Target data

Function

Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation (By similarity). Isoform 2 probably acts as a scaffold for specialized synapses.

Alternative names

C-terminal-binding protein 2, CtBP2, CTBP2

Recommended products

Rabbit Recombinant Monoclonal CTBP2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7611(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248197 is the carrier-free version of Anti-CTBP2 antibody [EPR7611(B)] ab128871.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CTBP2 also known as C-terminal binding protein 2 is a transcriptional co-repressor involved in regulating gene expression. It is a protein with a molecular mass of approximately 48 kDa. CTBP2 is expressed in various tissues with high levels in neural muscular and epithelial cells. Mechanically CTBP2 operates by interacting with transcription factors and histone-modifying enzymes modulating chromatin structure to control access to DNA.

Biological function summary

CTBP2 plays a significant role in cellular processes such as development and differentiation. It frequently acts as part of multi-protein complexes interacting with diverse partners to facilitate transcriptional repression. These interactions are essential for balancing gene activity ensuring appropriate tissue development and function. CTBP2 influences cellular dynamics by regulating target genes implicated in growth survival and metabolic pathways.

Pathways

CTBP2 integrates into significant signaling pathways like Wnt and TGF-beta where it reinforces regulatory control by interacting with critical factors such as beta-catenin or Smad proteins. It collaborates with other proteins like CTBP1 dictating cellular responses and influencing pathophysiological processes. By coordinating these pathways CTBP2 supports cellular homeostasis impacting tissues in response to external and internal cues.

Associated diseases and disorders

CTBP2 has associations with cancer and neurodegenerative diseases. Its overexpression or altered activity links to cancer progression as it promotes a transcriptional environment conducive to tumor growth. Proteins such as E-cadherin connect with CTBP2 in metastasis exemplifying its role in cancer cell dissemination. Additionally changes in CTBP2 function relate to neurodegenerative conditions affecting nerve cell survival and synaptic activity pointing to its involvement in neuronal health and disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)

    This data was developed using Anti-CTBP2 antibody [EPR7611(B)] ab128871, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling CTBP2 with purified Anti-CTBP2 antibody [EPR7611(B)] ab128871 at 1:50 dilution (2.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)

    This data was developed using Anti-CTBP2 antibody [EPR7611(B)] ab128871, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling CTBP2 with purified Anti-CTBP2 antibody [EPR7611(B)] ab128871 at 1:12000 (0.011 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197), expandable thumbnail

    Western blot - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)

    This data was developed using Anti-CTBP2 antibody [EPR7611(B)] ab128871, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-CTBP2 antibody [EPR7611(B)] (Anti-CTBP2 antibody [EPR7611(B)] ab128871) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Mouse brain lysate at 20 µg

    Lane 4: Rat brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa

    Observed band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)

    This data was developed using Anti-CTBP2 antibody [EPR7611(B)] ab128871, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling CTBP2 with purified Anti-CTBP2 antibody [EPR7611(B)] ab128871 at 1:6000 (0.021 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Flow Cytometry (Intracellular) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)

    This data was developed using Anti-CTBP2 antibody [EPR7611(B)] ab128871, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling CTBP2 with purified Anti-CTBP2 antibody [EPR7611(B)] ab128871 at 1/20 dilution (5 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)

    This data was developed using Anti-CTBP2 antibody [EPR7611(B)] ab128871, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling CTBP2 with purified Anti-CTBP2 antibody [EPR7611(B)] ab128871 at 1:12000 (0.011 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)

    This data was developed using Anti-CTBP2 antibody [EPR7611(B)] ab128871, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling CTBP2 with purified Anti-CTBP2 antibody [EPR7611(B)] ab128871 at 1:6000 (0.021 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

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