Rabbit Recombinant Monoclonal DIAC antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-CTBS antibody [EPR29368-527] - BSA and Azide free (Detector) (AB320695), expandable thumbnail](https://content.abcam.com/products/images/ctbs-antibody-epr29368-527-bsa-and-azide-free-detector-ab320695--sandwich-elisa-img434196.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-CTBS antibody [EPR29368-527] - BSA and Azide free (Detector) (AB320695)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Involved in the degradation of asparagine-linked glycoproteins. Hydrolyze of N-acetyl-beta-D-glucosamine (1-4)N-acetylglucosamine chitobiose core from the reducing end of the bond, it requires prior cleavage by glycosylasparaginase.
Alternative names
Di-N-acetylchitobiase
Recommended products
Rabbit Recombinant Monoclonal DIAC antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
Di-N-acetylchitobiase
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR29368-527
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab320695 is a BSA and Azide free antibody supplied in an unconjugated
format and it is suitable for sandwich ELISAs to quantify Human CTBS.
The recommended pair for sandwich ELISA is:
Capture: Anti-CTBS antibody [EPR29368-575] - BSA and Azide free (Capture/Detector) ab320694, Human CTBS Capture Antibody (unconjugated)
Detector: ab320695, Human CTBS Detector Antibody (unconjugated)
The reference range value is 15.63-2000 pg/mL.
The recommended antibody orientation is based on internal optimization
for ELISAbased assays. Antibody orientation is assay dependent and
needs to be optimized for each assay type. Please note that the
range provided for this antibody is only an estimation based on the
performance of the product using the recommended antibody pair.
Performance of the antibody pair will depend on the specific
characteristics of your assay. We guarantee the product works in
sandwich ELISA, but we do not guarantee the sensitivity or dynamic
range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA,
sodium azide and glycerol for higher conjugation efficiency.
The antibodies are provided at an approximate concentration of
1 mg/ml as measured by the protein A280 method. Use our conjugation
kits for antibody conjugates that are ready-to-use in as
little as 20 minutes with <1 minute hands-on-time and 100% antibody
recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- Activity summary
CTBS also known as chitobiase is a lysosomal enzyme that plays a role in the breakdown of chitinous substances. The protein has an approximate mass of 55 kDa. It is expressed mainly in tissues involved in the degradation processes such as the liver kidney and spleen. CTBS facilitates the hydrolysis of chitobiose into its monosaccharide components. This enzymatic activity is important for regulating cellular turnover and recycling materials within the lysosome.
- Biological function summary
CTBS participates in the catabolism of glycoproteins and glycolipids contributing to the proper functioning of lysosomal degradation. The protein does not form a part of a complex but functions independently within the lysosome to assist in processing lysosomal storage materials. The activity of CTBS influences cellular homeostasis and the elimination of unnecessary macromolecular structures. Proper CTBS function prevents accumulation of partially degraded molecules that might disrupt cellular operations.
- Pathways
The CTBS enzyme acts significantly within the lysosomal degradation pathway. This pathway involves the breakdown and recycling of various biomolecules which is important in maintaining metabolic balance. CTBS interacts with other enzymes like hexosaminidases which further process the breakdown products of its enzymatic activity. The lysosomal degradation pathway plays a role in cellular nutrition and waste elimination ensuring cells receive necessary substrates while disposing of metabolic waste.
- Associated diseases and disorders
CTBS has links to lysosomal storage disorders. These diseases occur when substrates accumulate within the lysosome due to deficient enzymatic activity leading to cellular dysfunction. One notable disorder related to CTBS dysfunction is Sandhoff disease where deficient enzyme activity causes buildup of glycosphingolipids. In this context CTBS is associated with proteins like hexosaminidase A which cooperatively contribute to lysosomal degradation processes. Understanding the role of CTBS in these disorders can help develop therapeutic strategies to manage or treat the conditions associated with lysosomal malfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-CTBS antibody [EPR29368-527] - BSA and Azide free (Detector) (ab320695), expandable thumbnail](https://content.abcam.com/products/images/ctbs-antibody-epr29368-527-bsa-and-azide-free-detector-ab320695--sandwich-elisa-img434196.jpg "Sandwich ELISA - Anti-CTBS antibody [EPR29368-527] - BSA and Azide free (Detector) (ab320695)")
Sandwich ELISA - Anti-CTBS antibody [EPR29368-527] - BSA and Azide free (Detector) (ab320695)
Sandwich ELISA of Human CTBS Antibody Pair - BSA and Azide free ab320693 with the capture antibody Anti-CTBS antibody [EPR29368-575] - BSA and Azide free (Capture/Detector) ab320694 dilution at 2 ug/mL and detector antibody ab320695 dilution at 0.5 ug/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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