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AB327168

Anti-CTCFL Antibody

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Rabbit Polyclonal BORIS antibody. Suitable for IHC-P, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CTCFL.

View Alternative Names

BORIS, CTCFL, Transcriptional repressor CTCFL, Brother of the regulator of imprinted sites, CCCTC-binding factor, CTCF paralog, CTCF-like protein, Cancer/testis antigen 27, Zinc finger protein CTCF-T, CT27

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCFL Antibody (AB327168)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCFL Antibody (AB327168)

Immunohistochemical staining of human testis using ab327168 at 1 : 200–1 : 500 dilution, showing moderate nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCFL Antibody (AB327168)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCFL Antibody (AB327168)

Immunohistochemistry analysis in human testis and skeletal muscle tissues using ab327168 at 1 : 200–1 : 500 dilution. Corresponding CTCFL RNA-seq data are presented for the same tissues.

ChIP - Anti-CTCFL Antibody (AB327168)
  • ChIP

Supplier Data

ChIP - Anti-CTCFL Antibody (AB327168)

ChIP-Exo-Seq composite graph for ab327168 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCFL Antibody (AB327168)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCFL Antibody (AB327168)

Immunohistochemical staining of human skeletal muscle using ab327168 at 1 : 200–1 : 500 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ChIP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CTCFL.

Q8NI51

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
See full target information CTCFL
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Product promise

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