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AB228897

Anti-CTF/NFIA antibody

5

(1 Review)

|

(12 Publications)

Rabbit Polyclonal NFIA antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NFIA.

View Alternative Names

KIAA1439, NFIA, Nuclear factor 1 A-type, NF1-A, Nuclear factor 1/A, CCAAT-box-binding transcription factor, Nuclear factor I/A, TGGCA-binding protein, CTF, NF-I/A, NFI-A

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-CTF/NFIA antibody (AB228897)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CTF/NFIA antibody (AB228897)

4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for CTF/NFIA (green) using ab228897 at 1/1000 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)

Paraffin-embedded rat hindbrain tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Frozen sections) - Anti-CTF/NFIA antibody (AB228897)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CTF/NFIA antibody (AB228897)

Frozen section of adult mouse retina tissue stained for CTF/NFIA using ab228897 at 1/250 dilution in immunohistochemical analysis.

Nuclear counterstain : Fluoroshield with DAPI (blue). Beta-Tubulin is detected with an anti-Tubulin 3/TUJ1 antibody (red) at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)

Paraffin-embedded mouse muscle tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (AB228897)

Paraffin-embedded mouse liver tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-CTF/NFIA antibody (AB228897)
  • WB

Supplier Data

Western blot - Anti-CTF/NFIA antibody (AB228897)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-CTF/NFIA antibody (ab228897) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-Fr, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NFIA. The exact immunogen used to generate this antibody is proprietary information.

Q12857

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transcription factor CTF/NFIA also known simply as NFIA plays a significant role in gene regulation. It has a molecular weight of approximately 55 kDa. CTF/NFIA is expressed in various tissues with a higher abundance in the central nervous system particularly during development. It binds to specific DNA sequences influencing the transcription of target genes essential for developmental processes and cellular differentiation.
Biological function summary

CTF/NFIA participates in several cellular processes by forming part of a complex that regulates transcription. This complex influences the expression of genes involved in neurodevelopment and gliogenesis. CTF/NFIA acts as a major regulator controlling genes important for the proper formation of brain structure and function. Its ability to modulate gene expression allows it to act as a mediator for cellular environments that require precise genetic control.

Pathways

The protein integrates a network involving the Wnt signaling pathway and the Hedgehog signaling pathway. These pathways are fundamental in maintaining proper cellular growth division and differentiation. In the Wnt signaling pathway CTF/NFIA interacts with the β-catenin complex to control the expression of genes related to cell fate decisions. Similarly within the Hedgehog signaling pathway CTF/NFIA helps in modulating transcriptional responses to developmental cues.

CTF/NFIA has significant implications for brain-related conditions including hydrocephalus and intellectual disabilities. Mutations or dysregulation of NFIA are linked to structural brain malformations that can have severe impacts. Additionally CTF/NFIA interacts with proteins like GLI1 in the context of disease further highlighting its role in signal transduction pathways that when disrupted contribute to these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
See full target information NFIA
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Molecular and clinical oncology 23:72 PubMed40599718

2025

Nuclear factor IA-mediated transcriptional regulation of crystallin αB inhibits hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Jin,Pingping Hu,Yihe Dai,Wenchao Gu,Jiang Han,Haihan Song

eLife 13: PubMed40085500

2025

Dual role of FOXG1 in regulating gliogenesis in the developing neocortex via the FGF signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mahima Bose,Ishita Talwar,Varun Suresh,Urvi Mishra,Shiona Biswas,Anuradha Yadav,Shital T Suryavanshi,Simon Hippenmeyer,Shubha Tole

Neurochemical research 50:77 PubMed39789409

2025

Identification of a Subpopulation of Astrocyte Progenitor Cells in the Neonatal Subventricular Zone: Evidence that Migration is Regulated by Glutamate Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zila Martinez-Lozada,Alain M Guillem,Isabella Song,Michael V Gonzalez,Hajime Takano,Esha Parikh,Jeffrey D Rothstein,Mary E Putt,Michael B Robinson

Nature cell biology 27:347-359 PubMed39779941

2025

Mapping the developmental trajectory of human astrocytes reveals divergence in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin Sojka,Hsiao-Lin V Wang,Tarun N Bhatia,Yangping Li,Pankaj Chopra,Anson Sing,Anna Voss,Alexia King,Feng Wang,Kevin Joseph,Vidhya M Ravi,Jeffrey Olson,Kimberly Hoang,Edjah Nduom,Victor G Corces,Bing Yao,Steven A Sloan

Journal of neuroinflammation 20:247 PubMed37880726

2023

Cell-specific NFIA upregulation promotes epileptogenesis by TRPV4-mediated astrocyte reactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Kong,Tao-Xiang Chen,Xiang-Lei Jia,Xue-Lei Cheng,Meng-Liu Zeng,Jing-Yi Liang,Xiao-Hua He,Jun Yin,Song Han,Wan-Hong Liu,Yuan-Teng Fan,Ting Zhou,Yu-Min Liu,Bi-Wen Peng

iScience 26:107887 PubMed37771660

2023

Acquisition of neural fate by combination of BMP blockade and chromatin modification.

Applications

Unspecified application

Species

Unspecified reactive species

Agnes Lee Chen Ong,Toshiya Kokaji,Arisa Kishi,Yoshihiro Takihara,Takuma Shinozuka,Ren Shimamoto,Ayako Isotani,Manabu Shirai,Noriaki Sasai

Nature communications 14:3372 PubMed37291151

2023

Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Molina-Gonzalez,Rebecca K Holloway,Zoeb Jiwaji,Owen Dando,Sarah A Kent,Katie Emelianova,Amy F Lloyd,Lindsey H Forbes,Ayisha Mahmood,Thomas Skripuletz,Viktoria Gudi,James A Febery,Jeffrey A Johnson,Jill H Fowler,Tanja Kuhlmann,Anna Williams,Siddharthan Chandran,Martin Stangel,Andrew J M Howden,Giles E Hardingham,Veronique E Miron

Frontiers in cardiovascular medicine 9:1062695 PubMed36568562

2022

Large-conductance Ca-activated K channel β1-subunit maintains the contractile phenotype of vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meili Wang,Shuanglei Li,Hongshan Liu,Mingyuan Liu,Jin Zhang,Yang Wu,Cangsong Xiao,Haixia Huang

Development (Cambridge, England) 149: PubMed36196585

2022

Dentate gyrus morphogenesis is regulated by β-catenin function in hem-derived fimbrial glia.

Applications

Unspecified application

Species

Unspecified reactive species

Arpan Parichha,Debarpita Datta,Varun Suresh,Mallika Chatterjee,Michael J Holtzman,Shubha Tole

Mediators of inflammation 2022:5400592 PubMed36254157

2022

miR-181d-5p Protects against Retinal Ganglion Cell Death after Blunt Ocular Injury by Regulating NFIA-Medicated Astrocyte Development.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghua Li,Junyi Liu,Yuanping Zhang,Xu Zha,Hong Zhang,Yongying Tang,Xueying Zhao
View all publications
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