Rabbit Polyclonal NFIA antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NFIA.
Images
Publications
Filter by
- Journal of neuroinflammation 20:2472023Cell-specific NFIA upregulation promotes epileptogenesis by TRPV4-mediated astrocyte reactivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuo Kong,Tao-Xiang Chen,Xiang-Lei Jia,Xue-Lei Cheng,Meng-Liu Zeng,Jing-Yi Liang,Xiao-Hua He,Jun Yin,Song Han,Wan-Hong Liu,Yuan-Teng Fan,Ting Zhou,Yu-Min Liu,Bi-Wen PengPubMed 37880726
- iScience 26:1078872023Acquisition of neural fate by combination of BMP blockade and chromatin modification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnes Lee Chen Ong,Toshiya Kokaji,Arisa Kishi,Yoshihiro Takihara,Takuma Shinozuka,Ren Shimamoto,Ayako Isotani,Manabu Shirai,Noriaki SasaiPubMed 37771660
- Nature communications 14:33722023Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene Molina-Gonzalez,Rebecca K Holloway,Zoeb Jiwaji,Owen Dando,Sarah A Kent,Katie Emelianova,Amy F Lloyd,Lindsey H Forbes,Ayisha Mahmood,Thomas Skripuletz,Viktoria Gudi,James A Febery,Jeffrey A Johnson,Jill H Fowler,Tanja Kuhlmann,Anna Williams,Siddharthan Chandran,Martin Stangel,Andrew J M Howden,Giles E Hardingham,Veronique E MironPubMed 37291151
- Development (Cambridge, England) 149:2022Dentate gyrus morphogenesis is regulated by β-catenin function in hem-derived fimbrial glia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArpan Parichha,Debarpita Datta,Varun Suresh,Mallika Chatterjee,Michael J Holtzman,Shubha TolePubMed 36196585
- Mediators of inflammation 2022:54005922022miR-181d-5p Protects against Retinal Ganglion Cell Death after Blunt Ocular Injury by Regulating NFIA-Medicated Astrocyte Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinghua Li,Junyi Liu,Yuanping Zhang,Xu Zha,Hong Zhang,Yongying Tang,Xueying ZhaoPubMed 36254157
- Nature communications 13:7662022Human transcription factor protein interaction networks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelka Göös,Matias Kinnunen,Kari Salokas,Zenglai Tan,Xiaonan Liu,Leena Yadav,Qin Zhang,Gong-Hong Wei,Markku VarjosaloPubMed 35140242
- Cell death & disease 11:9492020MiRNA-671-5p Promotes prostate cancer development and metastasis by targeting NFIA/CRYAB axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiguo Zhu,Lianmin Luo,Qian Xiang,Jiamin Wang,Yangzhou Liu,Yihan Deng,Zhigang ZhaoPubMed 33144585
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NFIA. The exact immunogen used to generate this antibody is proprietary information. Database link Q12857
Reactivity data
Select an application| WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Expected |
| Mouse | Expected | Tested | Expected | Tested |
| Rat | Expected | Tested | Expected | Expected |
| Chicken | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow | Dilution info - | Notes - |
Target data
Function
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Alternative names
KIAA1439, NFIA, Nuclear factor 1 A-type, NF1-A, Nuclear factor 1/A, CCAAT-box-binding transcription factor, Nuclear factor I/A, TGGCA-binding protein, CTF, NF-I/A, NFI-A
Recommended products
Rabbit Polyclonal NFIA antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NFIA.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NFIA. The exact immunogen used to generate this antibody is proprietary information. Database link Q12857
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The transcription factor CTF/NFIA also known simply as NFIA plays a significant role in gene regulation. It has a molecular weight of approximately 55 kDa. CTF/NFIA is expressed in various tissues with a higher abundance in the central nervous system particularly during development. It binds to specific DNA sequences influencing the transcription of target genes essential for developmental processes and cellular differentiation.
- Biological function summary
CTF/NFIA participates in several cellular processes by forming part of a complex that regulates transcription. This complex influences the expression of genes involved in neurodevelopment and gliogenesis. CTF/NFIA acts as a major regulator controlling genes important for the proper formation of brain structure and function. Its ability to modulate gene expression allows it to act as a mediator for cellular environments that require precise genetic control.
- Pathways
The protein integrates a network involving the Wnt signaling pathway and the Hedgehog signaling pathway. These pathways are fundamental in maintaining proper cellular growth division and differentiation. In the Wnt signaling pathway CTF/NFIA interacts with the β-catenin complex to control the expression of genes related to cell fate decisions. Similarly within the Hedgehog signaling pathway CTF/NFIA helps in modulating transcriptional responses to developmental cues.
- Associated diseases and disorders
CTF/NFIA has significant implications for brain-related conditions including hydrocephalus and intellectual disabilities. Mutations or dysregulation of NFIA are linked to structural brain malformations that can have severe impacts. Additionally CTF/NFIA interacts with proteins like GLI1 in the context of disease further highlighting its role in signal transduction pathways that when disrupted contribute to these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Western blot - Anti-CTF/NFIA antibody (ab228897)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-CTF/NFIA antibody (ab228897) at 1/1000 dilution
All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa
Immunohistochemistry (Frozen sections) - Anti-CTF/NFIA antibody (ab228897)
Frozen section of adult mouse retina tissue stained for CTF/NFIA using ab228897 at 1/250 dilution in immunohistochemical analysis.
Nuclear counterstain: Fluoroshield with DAPI (blue). Beta-Tubulin is detected with an anti-Tubulin 3/TUJ1 antibody (red) at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CTF/NFIA antibody (ab228897)
4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for CTF/NFIA (green) using ab228897 at 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (ab228897)
Paraffin-embedded mouse liver tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (ab228897)
Paraffin-embedded mouse muscle tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (ab228897)
Paraffin-embedded rat hindbrain tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com