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AB220288

Anti-CTF18 antibody

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(1 Publication)

Rabbit Polyclonal CTF18 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CHTF18 aa 850 to C-terminus.

View Alternative Names

C16orf41, CTF18, CHTF18, Chromosome transmission fidelity protein 18 homolog, hCTF18, CHL12

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CTF18 antibody (AB220288)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CTF18 antibody (AB220288)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling CTF18 with ab220288 at 4 μg/ml (green).

Western blot - Anti-CTF18 antibody (AB220288)
  • WB

Supplier Data

Western blot - Anti-CTF18 antibody (AB220288)

All lanes:

Western blot - Anti-CTF18 antibody (ab220288) at 1/100 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Predicted band size: 107 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CHTF18 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WVB6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTF18 also known as Chromosome Transmission Fidelity 18 Homolog is a protein involved in DNA replication and repair. It has a molecular weight of approximately 112 kDa. CTF18 can be found in the nucleus of the cell where it interacts with other factors essential for maintaining genome integrity. Researchers identify CTF18 as a subunit of the Ctf18-RFC (Replication Factor C) complex which is key in the loading of PCNA (Proliferating Cell Nuclear Antigen) onto DNA during replication.
Biological function summary

CTF18 plays a significant role in chromosome cohesion during cell division. It forms part of the Ctf18-RFC complex which assists in the establishment of sister chromatid cohesion. This protein complex is important for the proper segregation of chromosomes during mitosis preventing genomic instability. The coordination and function of CTF18 with other cohesin complex proteins ensure the accurate distribution of genetic material into daughter cells.

Pathways

Studies show CTF18 involvement in the DNA damage response and repair pathways. It operates in tandem with proteins like RAD17 and RFC1 participating in DNA damage checkpoint activation and facilitating repair processes. CTF18 links directly to cell cycle control pathways contributing to the maintenance of genomic stability by supporting the DNA replication machinery’s response to damage and stress.

Researchers associate CTF18 with genomic instability-related conditions such as cancer and chromosomal instability syndromes. Alterations or deficiencies in CTF18 expression or function can lead to improper chromosome segregation and an increased risk of tumorigenesis. The connection between CTF18 and proteins like RAD51 which is pivotal for homologous recombination repair highlights the importance of CTF18 in maintaining cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. Interacts with and stimulates DNA polymerase POLH. During DNA repair synthesis, involved in loading DNA polymerase POLE at the sites of local damage (PubMed : 20227374).
See full target information CHTF18
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The FEBS journal 292:1950-1971 PubMed39871113

2025

Transcriptomic signatures and network-based methods uncover new senescent cell anti-apoptotic pathways and senolytics.

Applications

Unspecified application

Species

Unspecified reactive species

Samael Olascoaga,Mina Konigsberg,Jesús Espinal-Enríquez,Hugo Tovar,Félix Matadamas-Martínez,Jaime Pérez-Villanueva,Norma Edith López-Diazguerrero
View all publications
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Product promise

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