Rabbit Polyclonal CTF18 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CHTF18 aa 850 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CHTF18 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WVB6
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. Interacts with and stimulates DNA polymerase POLH. During DNA repair synthesis, involved in loading DNA polymerase POLE at the sites of local damage (PubMed:20227374).
Alternative names
C16orf41, CTF18, CHTF18, Chromosome transmission fidelity protein 18 homolog, hCTF18, CHL12
Recommended products
Rabbit Polyclonal CTF18 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CHTF18 aa 850 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CHTF18 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WVB6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CTF18 also known as Chromosome Transmission Fidelity 18 Homolog is a protein involved in DNA replication and repair. It has a molecular weight of approximately 112 kDa. CTF18 can be found in the nucleus of the cell where it interacts with other factors essential for maintaining genome integrity. Researchers identify CTF18 as a subunit of the Ctf18-RFC (Replication Factor C) complex which is key in the loading of PCNA (Proliferating Cell Nuclear Antigen) onto DNA during replication.
- Biological function summary
CTF18 plays a significant role in chromosome cohesion during cell division. It forms part of the Ctf18-RFC complex which assists in the establishment of sister chromatid cohesion. This protein complex is important for the proper segregation of chromosomes during mitosis preventing genomic instability. The coordination and function of CTF18 with other cohesin complex proteins ensure the accurate distribution of genetic material into daughter cells.
- Pathways
Studies show CTF18 involvement in the DNA damage response and repair pathways. It operates in tandem with proteins like RAD17 and RFC1 participating in DNA damage checkpoint activation and facilitating repair processes. CTF18 links directly to cell cycle control pathways contributing to the maintenance of genomic stability by supporting the DNA replication machinery’s response to damage and stress.
- Associated diseases and disorders
Researchers associate CTF18 with genomic instability-related conditions such as cancer and chromosomal instability syndromes. Alterations or deficiencies in CTF18 expression or function can lead to improper chromosome segregation and an increased risk of tumorigenesis. The connection between CTF18 and proteins like RAD51 which is pivotal for homologous recombination repair highlights the importance of CTF18 in maintaining cellular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-CTF18 antibody (ab220288)
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling CTF18 with ab220288 at 4 μg/ml (green).
Western blot - Anti-CTF18 antibody (ab220288)
All lanes: Western blot - Anti-CTF18 antibody (ab220288) at 1/100 dilution
Lane 1: RT-4 cell lysate
Lane 2: U-251 MG cell lysate
Predicted band size: 107 kDa
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