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AB227180

Anti-CTGF antibody

4

(1 Review)

|

(7 Publications)

Rabbit Polyclonal CTGF antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CCN2 aa 200-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CTGF, HCS24, IGFBP8, CCN2, CCN family member 2, Cellular communication network factor 2, Connective tissue growth factor, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTGF antibody (AB227180)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTGF antibody (AB227180)

Formalin-fixed, paraffin-embedded human laryngeal cancer tissue stained for CTFG using ab227180 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTGF antibody (AB227180)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTGF antibody (AB227180)

Formalin-fixed, paraffin-embedded rat heart tissue stained for CTFG using ab227180 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.

Western blot - Anti-CTGF antibody (AB227180)
  • WB

Supplier Data

Western blot - Anti-CTGF antibody (AB227180)

All lanes:

Western blot - Anti-CTGF antibody (ab227180) at 1/300 dilution

All lanes:

Mouse lung lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/5000 dilution

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CCN2 aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P29279

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTGF also known as Connective Tissue Growth Factor is a significant protein in the CCN family involved in regulating various cellular functions. CTGF has a molecular weight of approximately 36-38 kDa. It is highly expressed in tissues such as the skin kidney and vascular tissues. This protein is secreted and can be found in the extracellular matrix where it interacts with other cellular components to mediate its effects.
Biological function summary

Connective Tissue Growth Factor plays an important role in cell adhesion proliferation and differentiation. It operates as an independent protein but can also be part of the extracellular matrix structure. CTGF functions as a mediator in the interaction between cells and their surrounding environment influencing processes such as angiogenesis and wound healing through these interactions.

Pathways

CTGF fits into the TGF-beta and Wnt signaling pathways where it helps modulate cellular responses to external stimuli. It interacts with proteins like fibronectin and integrins within these pathways enhancing cellular communication and response. These pathways are fundamental for both development and pathological conditions showing CTGF's influence on various biological processes.

CTGF shows a strong connection with fibrosis and cancer. In fibrotic conditions the CTGF protein interacts with collagen and fibronectin leading to excessive deposition in tissues. In cancers CTGF may promote tumor progression by facilitating angiogenesis and promoting invasive behaviors in tumors. These interactions highlight CTGF's role as a target for therapeutic strategies aimed at treating these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Is involved in the stimulation of osteoblast differentiation and has a critical role in osteogenesis (PubMed : 39414788). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
See full target information CCN2
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw6926 PubMed40845109

2025

Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Yu,Mincheng Yu,Peiyi Xie,Lei Guo,Yufei Zhao,Wenxin Xu,Xian Li,Mengyuan Wu,Zihao Zhang,Zheng Chen,Yongsheng Xiao,Jian Zhou,Jia Fan,Mien-Chie Hung,Yongfeng Xu,Bo Zhang,Qinghai Ye,Hui Li

Drug development research 83:1270-1280 PubMed35672933

2022

Ombuin ameliorates diabetic nephropathy in rats by anti-inflammation and antifibrosis involving Notch 1 and PPAR γ signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Liu,Caichun Deng,Ping Tan

Biochimica et biophysica acta. Molecular basis of disease 1868:166278 PubMed34601016

2021

Endothelial cell-derived pro-fibrotic factors increase TGF-β1 expression by smooth muscle cells in response to cycles of hypoxia-hyperoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Ismaeel,Dimitrios Miserlis,Evlampia Papoutsi,Gleb Haynatzki,William T Bohannon,Robert S Smith,Jack L Eidson,George P Casale,Iraklis I Pipinos,Panagiotis Koutakis

Cells 10: PubMed34440643

2021

Concurrent BMP Signaling Maintenance and TGF-β Signaling Inhibition Is a Hallmark of Natural Resistance to Muscle Atrophy in the Hibernating Bear.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Cussonneau,Christian Boyer,Charlotte Brun,Christiane Deval,Emmanuelle Loizon,Emmanuelle Meugnier,Elise Gueret,Emeric Dubois,Daniel Taillandier,Cécile Polge,Daniel Béchet,Guillemette Gauquelin-Koch,Alina L Evans,Jon M Arnemo,Jon E Swenson,Stéphane Blanc,Chantal Simon,Etienne Lefai,Fabrice Bertile,Lydie Combaret

Molecular medicine reports 23: PubMed33760134

2021

Islet transplantation ameliorates diabetes-induced testicular interstitial fibrosis and is associated with inhibition of TGF-β1/Smad2 pathway in a rat model of type 1 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Cai Zheng,Yi-Ling Feng,Yi-Hong Wang,Li-Jun Kong,Ming-Shi Zhou,Min-Min Wu,Cheng-Yang Liu,Hua-Chun Weng,Hong-Wei Wang

International journal of molecular medicine 44:927-938 PubMed31257476

2019

Effects of high glucose on proliferation and function of circulating fibrocytes: Involvement of CXCR4/SDF‑1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yingzheng Weng,Jiangjie Lou,Xiaowei Liu,Senna Lin,Chenkai Xu,Changqing Du,Lijiang Tang

Biomedicine & pharmacotherapy = Biomedecine & phar 111:1429-1437 PubMed30841458

2019

CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Mei Song,Fang Liu,Yan-Ming Chen,Yi-Jing Liu,Xiao-Di Wang,Shi-Yu Du
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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