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AB5097

Anti-CTGF antibody

4

(8 Reviews)

|

(24 Publications)

Rabbit Polyclonal CTGF antibody. Suitable for ICC, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Recombinant fragment - Human, Rat, Pig samples. Cited in 24 publications.

View Alternative Names

CTGF, HCS24, IGFBP8, CCN2, CCN family member 2, Cellular communication network factor 2, Connective tissue growth factor, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8

3 Images
Immunocytochemistry - Anti-CTGF antibody (AB5097)
  • ICC

Lab

Immunocytochemistry - Anti-CTGF antibody (AB5097)

ab5097 staining CTGF in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab5097 at 1μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Immunocytochemistry - Anti-CTGF antibody (AB5097)
  • ICC

AbReview34748****

Immunocytochemistry - Anti-CTGF antibody (AB5097)

ab5097 staining CTGF in Human Hela, cervix cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. A Cy3®-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/800).

This image is courtesy of an Abreview submitted by Beate Thode

Western blot - Anti-CTGF antibody (AB5097)
  • WB

Unknown

Western blot - Anti-CTGF antibody (AB5097)

Western blot, on recombinant Human CTGF produced in E. coli, using ab5097 at 1/500.

Not yet tested against endogenous CTGF

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-CTGF antibody (ab5097) at 1/500 dilution

Lane 1:

recombinant human CTGF at 0.01 µg

Lane 2:

recombinant human CTGF at 0.01 µg with Human CTGF peptide (<a href='/en-us/products/proteins-peptides/human-ctgf-peptide-ab13741'>ab13741</a>)

Lane 3:

recombinant human CTGF at 0.1 µg

Lane 4:

recombinant human CTGF at 0.1 µg with Human CTGF peptide (<a href='/en-us/products/proteins-peptides/human-ctgf-peptide-ab13741'>ab13741</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig, Rat

Applications

ICC/IF, IHC-Fr, WB, IHC-P, ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects recombinant human CTGF by Western Blotting. The band can be blocked by the immunising peptide (ab13741&#41;. We have been unable to detect endogenous protein in WB with ab5097&#46;

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p>This application has only been tested on recombinant protein.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTGF also known as Connective Tissue Growth Factor is a significant protein in the CCN family involved in regulating various cellular functions. CTGF has a molecular weight of approximately 36-38 kDa. It is highly expressed in tissues such as the skin kidney and vascular tissues. This protein is secreted and can be found in the extracellular matrix where it interacts with other cellular components to mediate its effects.
Biological function summary

Connective Tissue Growth Factor plays an important role in cell adhesion proliferation and differentiation. It operates as an independent protein but can also be part of the extracellular matrix structure. CTGF functions as a mediator in the interaction between cells and their surrounding environment influencing processes such as angiogenesis and wound healing through these interactions.

Pathways

CTGF fits into the TGF-beta and Wnt signaling pathways where it helps modulate cellular responses to external stimuli. It interacts with proteins like fibronectin and integrins within these pathways enhancing cellular communication and response. These pathways are fundamental for both development and pathological conditions showing CTGF's influence on various biological processes.

CTGF shows a strong connection with fibrosis and cancer. In fibrotic conditions the CTGF protein interacts with collagen and fibronectin leading to excessive deposition in tissues. In cancers CTGF may promote tumor progression by facilitating angiogenesis and promoting invasive behaviors in tumors. These interactions highlight CTGF's role as a target for therapeutic strategies aimed at treating these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Is involved in the stimulation of osteoblast differentiation and has a critical role in osteogenesis (PubMed : 39414788). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
See full target information CCN2
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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Genome medicine 16:98 PubMed39138551

2024

Spatial multiomics reveals a subpopulation of fibroblasts associated with cancer stemness in human hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Yu Jing,Dan Liu,Na Feng,Hui Dong,He-Qi Wang,Xi Yan,Xu-Feng Chen,Min-Cheng Qu,Ping Lin,Bin Yi,Feiling Feng,Lei Chen,Hong-Yang Wang,Hong Li,Yu-Fei He

Oncology letters 25:71 PubMed36688109

2023

Long non-coding RNA PVT1 promotes the proliferation, migration and EMT process of ovarian cancer cells by regulating CTGF.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Dong,Huan Wang,Yun Gao,Shuai Wang,Weibo Wang

World journal of gastroenterology 28:547-569 PubMed35316957

2022

Connective tissue growth factor expression hints at aggressive nature of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ishrat Parveiz Bhat,Tahseen Bilal Rather,Irfan Maqbool,Gowhar Rashid,Kulsum Akhtar,Gulzar A Bhat,Fazl Q Parray,Besina Syed,Ishrat Younas Khan,Mohsin Kazi,Muhammad D Hussain,Mudassar Syed

International journal of molecular sciences 23: PubMed35008861

2021

Multi-Omics Profiling in Marfan Syndrome: Further Insights into the Molecular Mechanisms Involved in Aortic Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Judith M A Verhagen,Joyce Burger,Jos A Bekkers,Alexander T den Dekker,Jan H von der Thüsen,Marina Zajec,Hennie T Brüggenwirth,Marianne L T van der Sterre,Myrthe van den Born,Theo M Luider,Wilfred F J van IJcken,Marja W Wessels,Jeroen Essers,Jolien W Roos-Hesselink,Ingrid van der Pluijm,Ingrid M B H van de Laar,Erwin Brosens

Cancers 13: PubMed34885065

2021

Anti-Cancer Activity Profiling of Chemotherapeutic Agents in 3D Co-Cultures of Pancreatic Tumor Spheroids with Cancer-Associated Fibroblasts and Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

So-Dam Jang,Jeeyeun Song,Hyun-Ah Kim,Chang-Nim Im,Iftikhar Ali Khawar,Jong Kook Park,Hyo-Jeong Kuh

Molecular and cellular biochemistry 476:3353-3363 PubMed33942219

2021

Biomarkers and heterogeneous fibroblast phenotype associated with incisional hernia.

Applications

Unspecified application

Species

Unspecified reactive species

Finosh G Thankam,Nicholas K Larsen,Ann Varghese,Thao-Nguyen Bui,Matthew Reilly,Robert J Fitzgibbons,Devendra K Agrawal

Molecular medicine reports 23: PubMed33760200

2021

Connective tissue growth factor promotes retinal pigment epithelium mesenchymal transition via the PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yafen Wang,Tianfang Chang,Tong Wu,Wei Ye,Yusheng Wang,Guorui Dou,Hongjun Du,Yannian Hui,Changmei Guo

Connective tissue research 63:124-137 PubMed33535825

2021

Temporal changes in the muscle extracellular matrix due to volumetric muscle loss injury.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel B Hoffman,Christiana J Raymond-Pope,Jacob R Sorensen,Benjamin T Corona,Sarah M Greising

Cancer management and research 12:2577-2587 PubMed32346311

2020

MicroRNA-584 Impairs Cellular Proliferation and Sensitizes Osteosarcoma Cells to Cisplatin and Taxanes by Targeting CCN2.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Xiang'an Kong,Mousheng Zang,Bin Hu,Xing Fang,Binjie Gui,Yong Hu

Biochimica et biophysica acta. Gene regulatory mec 1862:194420 PubMed31476383

2019

CircINHA resists granulosa cell apoptosis by upregulating CTGF as a ceRNA of miR-10a-5p in pig ovarian follicles.

Applications

Unspecified application

Species

Unspecified reactive species

Tianya Guo,Jinbi Zhang,Wang Yao,Xing Du,QiQi Li,Long Huang,Menglan Ma,Qifa Li,Honglin Liu,Zengxiang Pan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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