Anti-CTGF antibody [EPR26521-6]- BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034)

This data was developed using ab305033, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labelling CTGF with ab305033 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : MCF7 (PMID : 10601320).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034)

This data was developed using ab305033, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labelling CTGF with ab305033 at 1/100 (4.68 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in Huh7 cell line. Negative control : U937 (predicted expression pattern from proteinatlas).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034)

This data was developed using ab305033, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HFF-1 (human skin fibroblast) cells labelling CTGF with ab305033 at 1/100 (4.68 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in HFF-1 cell line. Negative control : MCF7 (PMID : 10601320).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IP

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Immunoprecipitation - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034)

This data was developed using ab305033, the same antibody clone in a different buffer formulation.

CTGF was immunoprecipitated from 0.35 mg Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 ug with ab305033 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305033 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 ug

Lane 2 : ab305033 IP in Huh7 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305033 in Huh7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 41 seconds

The IP experiment was performed by ab305033 (YCA-R26521-3 HT H1L1 31101022205) (capture antibody), and band detected by another anti-CTGF antibody (ab209780)

ab305033 recognizes the native form of CTGF and is able to pull down and enrich the target protein.

All lanes:

Immunoprecipitation - Anti-CTGF antibody [EPR26521-6] (<a href='/en-us/products/primary-antibodies/ctgf-antibody-epr26521-6-ab305033'>ab305033</a>) at 1/30 dilution

Lane 1:

Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 ug

Lane 2:

<a href='/en-us/products/primary-antibodies/ctgf-antibody-epr26521-6-ab305033'>ab305033</a> IP in Huh7 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa

false

Exposure time: 41s

• Dot

Supplier Data

Dot Blot - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034)

This data was developed using ab305033, the same antibody clone in a different buffer formulation.

Dot blot analysis of CTGF using ab305033 at 1 : 1000 (0.468 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Exposure time : 3 minutes

Blocking and diluting buffer and concentration : 5% NFDM/TBST