Rabbit Recombinant Monoclonal CTGF antibody. Carrier free. Suitable for Dot, ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.
Images
![Immunoprecipitation - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034), expandable thumbnail](https://content.abcam.com/products/images/ctgf-antibody-epr26521-6-bsa-and-azide-free-ab305034--immunoprecipitation-img403443.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034)")
![Dot Blot - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034), expandable thumbnail](https://content.abcam.com/products/images/ctgf-antibody-epr26521-6-bsa-and-azide-free-ab305034--dot-blot-img403439.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034)")
![Flow Cytometry (Intracellular) - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034), expandable thumbnail](https://content.abcam.com/products/images/ctgf-antibody-epr26521-6-bsa-and-azide-free-ab305034--flow-cytometry-intracellular-img403440.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034)")
![Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034), expandable thumbnail](https://content.abcam.com/products/images/ctgf-antibody-epr26521-6-bsa-and-azide-free-ab305034--immunocytochemistry-immunofluorescence-img403441.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034)")
![Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034), expandable thumbnail](https://content.abcam.com/products/images/ctgf-antibody-epr26521-6-bsa-and-azide-free-ab305034--immunocytochemistry-immunofluorescence-img403442.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (AB305034)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Dot | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Alternative names
CTGF, HCS24, IGFBP8, CCN2, CCN family member 2, Cellular communication network factor 2, Connective tissue growth factor, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8
Recommended products
Rabbit Recombinant Monoclonal CTGF antibody. Carrier free. Suitable for Dot, ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26521-6
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CTGF also known as Connective Tissue Growth Factor is a significant protein in the CCN family involved in regulating various cellular functions. CTGF has a molecular weight of approximately 36-38 kDa. It is highly expressed in tissues such as the skin kidney and vascular tissues. This protein is secreted and can be found in the extracellular matrix where it interacts with other cellular components to mediate its effects.
- Biological function summary
Connective Tissue Growth Factor plays an important role in cell adhesion proliferation and differentiation. It operates as an independent protein but can also be part of the extracellular matrix structure. CTGF functions as a mediator in the interaction between cells and their surrounding environment influencing processes such as angiogenesis and wound healing through these interactions.
- Pathways
CTGF fits into the TGF-beta and Wnt signaling pathways where it helps modulate cellular responses to external stimuli. It interacts with proteins like fibronectin and integrins within these pathways enhancing cellular communication and response. These pathways are fundamental for both development and pathological conditions showing CTGF's influence on various biological processes.
- Associated diseases and disorders
CTGF shows a strong connection with fibrosis and cancer. In fibrotic conditions the CTGF protein interacts with collagen and fibronectin leading to excessive deposition in tissues. In cancers CTGF may promote tumor progression by facilitating angiogenesis and promoting invasive behaviors in tumors. These interactions highlight CTGF's role as a target for therapeutic strategies aimed at treating these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034), expandable thumbnail](https://content.abcam.com/products/images/ctgf-antibody-epr26521-6-bsa-and-azide-free-ab305034--immunoprecipitation-img403443.jpg "Immunoprecipitation - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034)")
Immunoprecipitation - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034)
This data was developed using Anti-CTGF antibody [EPR26521-6] ab305033, the same antibody clone in a different buffer formulation.
CTGF was immunoprecipitated from 0.35 mg Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 ug with Anti-CTGF antibody [EPR26521-6] ab305033 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CTGF antibody [EPR26521-6] ab305033 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 ug
Lane 2: Anti-CTGF antibody [EPR26521-6] ab305033 IP in Huh7 whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CTGF antibody [EPR26521-6] ab305033 in Huh7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds
The IP experiment was performed by Anti-CTGF antibody [EPR26521-6] ab305033 (YCA-R26521-3 HT H1L1 31101022205) (capture antibody), and band detected by another anti-CTGF antibody (Anti-CTGF antibody [EPR20728] ab209780).All lanes: Immunoprecipitation - Anti-CTGF antibody [EPR26521-6] (Anti-CTGF antibody [EPR26521-6] ab305033) at 1/30 dilution
Lane 1: Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 ug
Lane 2: Anti-CTGF antibody [EPR26521-6] ab305033 IP in Huh7 whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 41s
![Dot Blot - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034), expandable thumbnail](https://content.abcam.com/products/images/ctgf-antibody-epr26521-6-bsa-and-azide-free-ab305034--dot-blot-img403439.jpg "Dot Blot - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034)")
Dot Blot - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034)
This data was developed using Anti-CTGF antibody [EPR26521-6] ab305033, the same antibody clone in a different buffer formulation.
Dot blot analysis of CTGF using Anti-CTGF antibody [EPR26521-6] ab305033 at 1:1000 (0.468 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST![Flow Cytometry (Intracellular) - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034), expandable thumbnail](https://content.abcam.com/products/images/ctgf-antibody-epr26521-6-bsa-and-azide-free-ab305034--flow-cytometry-intracellular-img403440.jpg "Flow Cytometry (Intracellular) - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034)")
Flow Cytometry (Intracellular) - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034)
This data was developed using Anti-CTGF antibody [EPR26521-6] ab305033, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labelling CTGF with Anti-CTGF antibody [EPR26521-6] ab305033 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: MCF7 (PMID: 10601320).![Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034), expandable thumbnail](https://content.abcam.com/products/images/ctgf-antibody-epr26521-6-bsa-and-azide-free-ab305034--immunocytochemistry-immunofluorescence-img403441.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034)")
Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034)
This data was developed using Anti-CTGF antibody [EPR26521-6] ab305033, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HFF-1 (human skin fibroblast) cells labelling CTGF with Anti-CTGF antibody [EPR26521-6] ab305033 at 1/100 (4.68 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in HFF-1 cell line. Negative control: MCF7 (PMID: 10601320).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.![Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034), expandable thumbnail](https://content.abcam.com/products/images/ctgf-antibody-epr26521-6-bsa-and-azide-free-ab305034--immunocytochemistry-immunofluorescence-img403442.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034)")
Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [EPR26521-6]- BSA and Azide free (ab305034)
This data was developed using Anti-CTGF antibody [EPR26521-6] ab305033, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labelling CTGF with Anti-CTGF antibody [EPR26521-6] ab305033 at 1/100 (4.68 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in Huh7 cell line. Negative control: U937 (predicted expression pattern from proteinatlas).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
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