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Knockout Tested Rabbit Recombinant Multiclonal CTGF antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-CTGF antibody [RM1199] (AB318148), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (AB318148), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (AB318148), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (AB318148), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (AB318148), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-PIHC-FrIP
Human
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.

Alternative names

CTGF, HCS24, IGFBP8, CCN2, CCN family member 2, Cellular communication network factor 2, Connective tissue growth factor, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8

Recommended products

Knockout Tested Rabbit Recombinant Multiclonal CTGF antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
RM1199
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for mouse and rat FC.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CTGF also known as Connective Tissue Growth Factor is a significant protein in the CCN family involved in regulating various cellular functions. CTGF has a molecular weight of approximately 36-38 kDa. It is highly expressed in tissues such as the skin kidney and vascular tissues. This protein is secreted and can be found in the extracellular matrix where it interacts with other cellular components to mediate its effects.

Biological function summary

Connective Tissue Growth Factor plays an important role in cell adhesion proliferation and differentiation. It operates as an independent protein but can also be part of the extracellular matrix structure. CTGF functions as a mediator in the interaction between cells and their surrounding environment influencing processes such as angiogenesis and wound healing through these interactions.

Pathways

CTGF fits into the TGF-beta and Wnt signaling pathways where it helps modulate cellular responses to external stimuli. It interacts with proteins like fibronectin and integrins within these pathways enhancing cellular communication and response. These pathways are fundamental for both development and pathological conditions showing CTGF's influence on various biological processes.

Associated diseases and disorders

CTGF shows a strong connection with fibrosis and cancer. In fibrotic conditions the CTGF protein interacts with collagen and fibronectin leading to excessive deposition in tissues. In cancers CTGF may promote tumor progression by facilitating angiogenesis and promoting invasive behaviors in tumors. These interactions highlight CTGF's role as a target for therapeutic strategies aimed at treating these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-CTGF antibody [RM1199] (ab318148), expandable thumbnail

    Western blot - Anti-CTGF antibody [RM1199] (ab318148)

    Low expression: MCF7 and MOLT-4.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23139278 and PMID: 15955090).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CTGF antibody [RM1199] (ab318148) at 1/500 dilution

    Lane 1: Untreated Rat-1 (rat embryonic fibroblast) starved for 18 hours, whole cell lysate at 48 µg with NFDM/TBST

    Lane 2: Rat-1 starved for 18 hours, then treated with 10ng /ml TGF--β1 and 50μg/ml Heparin sodium salt for 24 hours, whole cell lysate at 48 µg with NFDM/TBST

    Lane 3: Untreated NIH/3T3 (mouse embryonic fibroblast) starved for 18 hours, whole cell lysate at 48 µg with NFDM/TBST

    Lane 4: NIH/3T3 starved for 18 hours, then treated with 10ng /ml TGF--β1 and 50μg/ml Heparin sodium salt for 24 hours, whole cell lysate at 48 µg with NFDM/TBST

    Lane 5: HFF-1 (human skin fibroblast) whole cell lysate at 48 µg with NFDM/TBST

    Lane 6: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 48 µg with NFDM/TBST

    Lane 7: Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 48 µg with NFDM/TBST

    Lane 8: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 48 µg with NFDM/TBST

    Lane 9: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 48 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 38 kDa, 18 kDa, 36 kDa

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (ab318148), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (ab318148)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling CTGF with ab318148 at 1/500 (1.03 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in HepG2 cells (shown in green) . The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Low expression: MOLT-4.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (ab318148), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (ab318148)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat-1 (rat embryonic fibroblast) cells labelling CTGF with ab318148 at 1/500 (1.03 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in Rat-1 cells (shown in green) after starvation 18 hours, then treatment with TGF beta 1(10 ng/ml) and Heparin sodium salt (50 ug/ml) for 24 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (ab318148), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (ab318148)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling CTGF with ab318148 at 1/500 (1.03 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in NIH/3T3 cells (shown in green) after starvation 18 hours, then treatment with TGF beta 1(10 ng/ml) and Heparin sodium salt (50 ug/ml) for 24 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (ab318148), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody [RM1199] (ab318148)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CTGF KO U-2 OS (CTGF knockout human bone osteosarcoma epithelial cell) cells labelling CTGF with ab318148 at 1/500 (1.03 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in wildtype U-2 OS cells (shown in green) and showing negative staining in CTGF knockout U-2 OS cells. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-CTGF antibody [RM1199] (ab318148), expandable thumbnail

    Western blot - Anti-CTGF antibody [RM1199] (ab318148)

    Lysates/proteins at 48 µg per lane.

    In Western blot, ab318148 was shown to bind specifically to CTGF. A band was observed at 18-38 kDa in wild-type U-2 OS cell lysates with whereas no signal observed at this size in CTGF knockout cell line.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CTGF antibody [RM1199] (ab318148) at 1/500 dilution

    Lane 1: Wild-type U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 48 µg with NFDM/TBST

    Lane 2: CTGF knockout U-2 OS whole cell lysate at 48 µg with NFDM/TBST

    Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 48 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 38 kDa, 18 kDa, 36 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-CTGF antibody [RM1199] (ab318148), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CTGF antibody [RM1199] (ab318148)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) (Right) / MCF7 (human breast adenocarcinoma epithelial cell) (Left) cells labelling CTGF with ab318148 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Low expression: MCF-7.

  • Flow Cytometry (Intracellular) - Anti-CTGF antibody [RM1199] (ab318148), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CTGF antibody [RM1199] (ab318148)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) (Right) / MOLT-4 (human lymphoblastic leukemia T lymphoblast) (Left) cells labelling CTGF with ab318148 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Low expression: MOLT-4.

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Product protocols

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