Rabbit Recombinant Monoclonal CTHRC1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Images
![Immunoprecipitation - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (AB259269), expandable thumbnail](https://content.abcam.com/products/images/cthrc1-antibody-epr22851-145-bsa-and-azide-free-ab259269--immunoprecipitation-img4100.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (AB259269)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (AB259269), expandable thumbnail](https://content.abcam.com/products/images/cthrc1-antibody-epr22851-145-bsa-and-azide-free-ab259269--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82430.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (AB259269)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (AB259269), expandable thumbnail](https://content.abcam.com/products/images/cthrc1-antibody-epr22851-145-bsa-and-azide-free-ab259269--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121084.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (AB259269)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (AB259269), expandable thumbnail](https://content.abcam.com/products/images/cthrc1-antibody-epr22851-145-bsa-and-azide-free-ab259269--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113952.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (AB259269)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
4 products for Alternative Product
1 products for Alternative Version
Target data
Function
May act as a negative regulator of collagen matrix deposition.
Alternative names
Collagen triple helix repeat-containing protein 1, UNQ762/PRO1550, CTHRC1
Recommended products
Rabbit Recombinant Monoclonal CTHRC1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Alternative names
Collagen triple helix repeat-containing protein 1, UNQ762/PRO1550, CTHRC1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR22851-145
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab259269 is the carrier-free version of Anti-CTHRC1 antibody [EPR22851-145] ab256458.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
The CTHRC1 protein also known as Collagen Triple Helix Repeat Containing 1 is a secreted protein with a mass of approximately 28 kDa. It plays a mechanical role in the extracellular matrix by modulating collagen fibrillogenesis and cross-linking. Increased expression of CTHRC1 protein often appears in areas undergoing active remodeling like those around wound healing sites fibrotic lesions and certain tumor tissues. The expression is not uniform across tissues and often corresponds to areas requiring structural reorganization or repair.
- Biological function summary
CTHRC1 impacts cell migration and invasion. It associates with several extracellular matrix components influencing cellular response through cytoskeletal reorganization. There is no evidence for its participation as a part of a larger protein complex. The modulation of collagen interactions by CTHRC1 enables it to play a significant role in angiogenesis and tissue repair processes affecting endothelial and fibroblast cell functions.
- Pathways
CTHRC1 participates in signaling pathways related to Transforming Growth Factor-beta (TGF-β) and Wnt. In the TGF-β pathway it impacts cellular responses to growth signals enhancing remodeling and repair mechanisms. Through the Wnt signaling pathway CTHRC1 interacts with Dishevelled proteins assisting in regulation and pathway activation related to cell proliferation and migration. These pathways highlight its involvement in important biological processes that influence tissue structure and cell communication.
- Associated diseases and disorders
CTHRC1 has relevance to both cancer progression and fibrotic diseases. Its overexpression is often noted in breast cancer where it drives tumor growth and metastasis interacting with other matrix components. Similarly in fibrosis CTHRC1 contributes to excessive collagen deposition and tissue stiffness. Proteins like TGF-β intersect with CTHRC1 functions further modulating disease pathway outcomes. Understanding CTHRC1's role in these disorders could inform therapeutic targets for modulating matrix dynamics.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269), expandable thumbnail](https://content.abcam.com/products/images/cthrc1-antibody-epr22851-145-bsa-and-azide-free-ab259269--immunoprecipitation-img4100.jpg "Immunoprecipitation - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269)")
Immunoprecipitation - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269)
CTHRC1 was immunoprecipitated from 0.35 mg SK-MEL-28 (Human malignant melanoma) whole cell lysate with Anti-CTHRC1 antibody [EPR22851-145] ab256458 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CTHRC1 antibody [EPR22851-145] ab256458 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SK-MEL-28 (Human malignant melanoma) whole cell lysate 10ug
Lane 2: Anti-CTHRC1 antibody [EPR22851-145] ab256458 IP in SK-MEL-28 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CTHRC1 antibody [EPR22851-145] ab256458 in SK-MEL-28 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTHRC1 antibody [EPR22851-145] ab256458).
All lanes: Immunoprecipitation - Anti-CTHRC1 antibody [EPR22851-145] (Anti-CTHRC1 antibody [EPR22851-145] ab256458)
Predicted band size: 26 kDa
Observed band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269), expandable thumbnail](https://content.abcam.com/products/images/cthrc1-antibody-epr22851-145-bsa-and-azide-free-ab259269--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82430.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269)
Immunohistochemical analysis of paraffin-embedded Human lung and lung carcinoma tissue labeling CTHRC1 with Anti-CTHRC1 antibody [EPR22851-145] ab256458 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). No staining on the paracancerous human lung tissue (panel A). Positive staining on human lung carcinoma (panel B; PMID: 29631554). The section was incubated with Anti-CTHRC1 antibody [EPR22851-145] ab256458 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTHRC1 antibody [EPR22851-145] ab256458).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269), expandable thumbnail](https://content.abcam.com/products/images/cthrc1-antibody-epr22851-145-bsa-and-azide-free-ab259269--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121084.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling CTHRC1 with Anti-CTHRC1 antibody [EPR22851-145] ab256458 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the stromal cells in the human colon carcinoma, nearly no staining on the paracancerous colon tissues (PMID: 24945147). The section was incubated with Anti-CTHRC1 antibody [EPR22851-145] ab256458 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTHRC1 antibody [EPR22851-145] ab256458).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269), expandable thumbnail](https://content.abcam.com/products/images/cthrc1-antibody-epr22851-145-bsa-and-azide-free-ab259269--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113952.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] - BSA and Azide free (ab259269)
Immunohistochemical analysis of paraffin-embedded Human breast and breast carcinoma tissue labeling CTHRC1 with Anti-CTHRC1 antibody [EPR22851-145] ab256458 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). No staining on the paracancerous human breast tissue (panel A). Positive staining on the stromal cells in the human breast carcinoma (panel B; PMID: 24945147). The section was incubated with Anti-CTHRC1 antibody [EPR22851-145] ab256458 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTHRC1 antibody [EPR22851-145] ab256458).
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