Mouse Recombinant Monoclonal Ctip1/BCL-11A antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (AB256127), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-123-bsa-and-azide-free-ab256127--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34388.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (AB256127)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (AB256127), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-123-bsa-and-azide-free-ab256127--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96483.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (AB256127)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (AB256127), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-123-bsa-and-azide-free-ab256127--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91614.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (AB256127)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (AB256127), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-123-bsa-and-azide-free-ab256127--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107405.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (AB256127)")
Publications
Filter by
- Blood 113:2765-37752008Molecular profiling of classical Hodgkin lymphoma tissues uncovers variations in the tumor microenvironment and correlations with EBV infection and outcome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBruno Chetaille,François Bertucci,Pascal Finetti,Benjamin Esterni,Aspasia Stamatoullas,Jean Michel Picquenot,Marie Christine Copin,Frank Morschhauser,Olivier Casasnovas,Tony Petrella,Thierry Molina,Anne Vekhoff,Pierre Feugier,Reda Bouabdallah,Daniel Birnbaum,Daniel Olive,Luc XerriPubMed 19096012
- Blood 111:3778-922008Novel markers of normal and neoplastic human plasmacytoid dendritic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeresa Marafioti,Jennifer C Paterson,Erica Ballabio,Kaaren K Reichard,Sara Tedoldi,Kevin Hollowood,Michael Dictor,Martin-Leo Hansmann,Stefano A Pileri,Martin J Dyer,Silvano Sozzani,Ivan Dikic,Andrey S Shaw,Tony Petrella,Harald Stein,Peter G Isaacson,Fabio Facchetti,David Y MasonPubMed 18218851
- Leukemia 20:1880-22006Gains of the proto-oncogene BCL11A and nuclear accumulation of BCL11A(XL) protein are frequent in primary mediastinal B-cell lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM A Weniger et. al.PubMed 16871282
- Leukemia 20:1439-412006The BCL11AXL transcription factor: its distribution in normal and malignant tissues and use as a marker for plasmacytoid dendritic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK Pulford et. al.PubMed 16710303
- Molecular cancer 5:182006Functional studies of BCL11A: characterization of the conserved BCL11A-XL splice variant and its interaction with BCL6 in nuclear paraspeckles of germinal center B cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Liu et. al.PubMed 16704730
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.52600-5.26000 µg/mL | Notes Human: Use at 5.26μg/ml.Mouse and rat: Use at 0.526μg/ml Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 0.52600-5.26000 µg/mL | Notes Human: Use at 5.26μg/ml.Mouse and rat: Use at 0.526μg/ml Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 0.52600-5.26000 µg/mL | Notes Human: Use at 5.26μg/ml.Mouse and rat: Use at 0.526μg/ml Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Transcription factor (PubMed:16704730, PubMed:29606353). Associated with the BAF SWI/SNF chromatin remodeling complex (PubMed:23644491). Binds to the 5'-TGACCA-3' sequence motif in regulatory regions of target genes, including a distal promoter of the HBG1 hemoglobin subunit gamma-1 gene (PubMed:29606353). Involved in regulation of the developmental switch from gamma- to beta-globin, probably via direct repression of HBG1; hence indirectly repressing fetal hemoglobin (HbF) level (PubMed:26375765, PubMed:29606353). Involved in brain development (PubMed:27453576). May play a role in hematopoiesis (By similarity). Essential factor in lymphopoiesis required for B-cell formation in fetal liver (By similarity). May function as a modulator of the transcriptional repression activity of NR2F2 (By similarity).
Alternative names
CTIP1, EVI9, KIAA1809, ZNF856, KIAA1809, ZNF856, EVI9, CTIP1, BCL11A, B-cell lymphoma/leukemia 11A, BCL-11A, B-cell CLL/lymphoma 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, Zinc finger protein 856, EVI-9
Recommended products
Mouse Recombinant Monoclonal Ctip1/BCL-11A antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
123
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab256127 is the carrier-free version of Anti-Ctip1/BCL-11A antibody [123] ab119275.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-123-bsa-and-azide-free-ab256127--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34388.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Ctip1/BCL-11A with Anti-Ctip1/BCL-11A antibody [123] ab119275 at 0.526μg/ml, followed by a ready to use secondary antibody. Nuclear staining on rat spleen is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.
The section was incubated with Anti-Ctip1/BCL-11A antibody [123] ab119275 for 30 mins at room temperature.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip1/BCL-11A antibody [123] ab119275).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-123-bsa-and-azide-free-ab256127--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96483.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Ctip1/BCL-11A with Anti-Ctip1/BCL-11A antibody [123] ab119275 at 5.26μg/ml, followed by a ready to use secondary antibody. Nuclear staining on human tonsil is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.
The section was incubated with Anti-Ctip1/BCL-11A antibody [123] ab119275 for 30 mins at room temperature.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip1/BCL-11A antibody [123] ab119275).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-123-bsa-and-azide-free-ab256127--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91614.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Ctip1/BCL-11A with Anti-Ctip1/BCL-11A antibody [123] ab119275 at 0.526μg/ml, followed by a ready to use secondary antibody. Nuclear staining on mouse cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.
The section was incubated with Anti-Ctip1/BCL-11A antibody [123] ab119275 for 30 mins at room temperature.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip1/BCL-11A antibody [123] ab119275).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-123-bsa-and-azide-free-ab256127--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107405.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Ctip1/BCL-11A with Anti-Ctip1/BCL-11A antibody [123] ab119275 at 5.26, followed by a ready to use secondary antibody. Nuclear staining on human breast carcinoma is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.
The section was incubated with Anti-Ctip1/BCL-11A antibody [123] ab119275 for 30 mins at room temperature.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip1/BCL-11A antibody [123] ab119275).
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