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Mouse Monoclonal Ctip1/BCL-11A antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse samples. Cited in 40 publications. Immunogen corresponding to Recombinant Protein within Human BCL11A.


Images

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Protein within Human BCL11A. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H165
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Tested
Tested
Mouse
Expected
Tested

Tested
Tested

Species

Human

Dilution info

0.1 µg for 106 Cells

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Target data

Function

Transcription factor (PubMed:16704730, PubMed:29606353). Associated with the BAF SWI/SNF chromatin remodeling complex (PubMed:23644491). Binds to the 5'-TGACCA-3' sequence motif in regulatory regions of target genes, including a distal promoter of the HBG1 hemoglobin subunit gamma-1 gene (PubMed:29606353). Involved in regulation of the developmental switch from gamma- to beta-globin, probably via direct repression of HBG1; hence indirectly repressing fetal hemoglobin (HbF) level (PubMed:29606353, PubMed:26375765). Involved in brain development (PubMed:27453576). May play a role in hematopoiesis (By similarity). Essential factor in lymphopoiesis required for B-cell formation in fetal liver (By similarity). May function as a modulator of the transcriptional repression activity of NR2F2 (By similarity).

Alternative names

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Mouse Monoclonal Ctip1/BCL-11A antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse samples. Cited in 40 publications. Immunogen corresponding to Recombinant Protein within Human BCL11A.

Alternative names

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Protein within Human BCL11A. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H165
Clone number

14B5

Purification technique

Multi-step chromatography

Specificity

Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for suitable positive controls.

Epitope

Epitope is in core of CTIP1/Bcl11a (aa's 172-434).

Light chain type

kappa

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

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Product promise

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4 product images

  • Flow Cytometry - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487), expandable thumbnail

    Flow Cytometry - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487)

    Overlay histogram showing Ramos cells stained with ab19487 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19487, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487), expandable thumbnail

    Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: Empty (0 μg)
    Lane 3: BCL11A (Ctip1) knockout HAP1 whole cell lysate (20 μg)
    Lane 4: Raji whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab19487 observed at 91 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab19487 was shown to specifically react with BCL11A (Ctip1) in HAP1 wild type cells. No band was observed when BCL11A (Ctip1) knockout cells were examined. Wild-type and BCL11A (Ctip1) knockout samples were subjected to SDS-PAGE. ab19487 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (AB19487)

    Predicted band size: 91 kDa

  • Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487), expandable thumbnail

    Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487)

    The CTIP1 / BCL-11A protein band is indicated at 92 kDa. This antibody also identified a 55 kDa protein band in Western blotting, principally in mouse fibroblasts (MEF), but this is unrelated to.

    Blocking was performed in 5% milk (in PBS). The primary antibody was diluted in 1% milk (in PBS) and incubated overnight at 4°C.

    All lanes: Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (AB19487)

    Lane 1: C6 Cell Line at 20 µg

    Lane 2: MEF Cell Line at 20 µg

    Lane 3: HDFn Cell LIne at 20 µg

    Lane 4: Jurkat Cell Line at 20 µg

    Lane 5: HEK293T Cell Line at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 91 kDa

  • Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487), expandable thumbnail

    Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (ab19487)

    The 120 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Ctip1 / BCL-11A.

    All lanes: Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (AB19487) at 1 µg/mL

    All lanes: Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (AB97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 91 kDa

    Observed band size: 120 kDa, 37 kDa, 42 kDa, 62 kDa

    Exposure time: 20min

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Product protocols

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