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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Mouse Monoclonal Ctip1/BCL-11A antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse samples. Cited in 40 publications. Immunogen corresponding to Recombinant Protein within Human BCL11A.
IgG1
Mouse
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES
Liquid
Monoclonal
Flow Cyt | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.1 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Transcription factor (PubMed:16704730, PubMed:29606353). Associated with the BAF SWI/SNF chromatin remodeling complex (PubMed:23644491). Binds to the 5'-TGACCA-3' sequence motif in regulatory regions of target genes, including a distal promoter of the HBG1 hemoglobin subunit gamma-1 gene (PubMed:29606353). Involved in regulation of the developmental switch from gamma- to beta-globin, probably via direct repression of HBG1; hence indirectly repressing fetal hemoglobin (HbF) level (PubMed:29606353, PubMed:26375765). Involved in brain development (PubMed:27453576). May play a role in hematopoiesis (By similarity). Essential factor in lymphopoiesis required for B-cell formation in fetal liver (By similarity). May function as a modulator of the transcriptional repression activity of NR2F2 (By similarity).
B-cell lymphoma/leukemia 11A, BCL-11A, B-cell CLL/lymphoma 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, Zinc finger protein 856, EVI-9, KIAA1809, ZNF856, EVI9, CTIP1, BCL11A
Mouse Monoclonal Ctip1/BCL-11A antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse samples. Cited in 40 publications. Immunogen corresponding to Recombinant Protein within Human BCL11A.
B-cell lymphoma/leukemia 11A, BCL-11A, B-cell CLL/lymphoma 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, Zinc finger protein 856, EVI-9, KIAA1809, ZNF856, EVI9, CTIP1, BCL11A
IgG1
Mouse
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES
Liquid
Monoclonal
14B5
Multi-step chromatography
Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for suitable positive controls.
Epitope is in core of CTIP1/Bcl11a (aa's 172-434).
kappa
Blue Ice
+4°C
+4°C
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Overlay histogram showing Ramos cells stained with ab19487 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19487, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: Empty (0 μg)
Lane 3: BCL11A (Ctip1) knockout HAP1 whole cell lysate (20 μg)
Lane 4: Raji whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab19487 observed at 91 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab19487 was shown to specifically react with BCL11A (Ctip1) in HAP1 wild type cells. No band was observed when BCL11A (Ctip1) knockout cells were examined. Wild-type and BCL11A (Ctip1) knockout samples were subjected to SDS-PAGE. ab19487 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (AB19487)
Predicted band size: 91 kDa
The CTIP1 / BCL-11A protein band is indicated at 92 kDa. This antibody also identified a 55 kDa protein band in Western blotting, principally in mouse fibroblasts (MEF), but this is unrelated to.
Blocking was performed in 5% milk (in PBS). The primary antibody was diluted in 1% milk (in PBS) and incubated overnight at 4°C.
All lanes: Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (AB19487)
Lane 1: C6 Cell Line at 20 µg
Lane 2: MEF Cell Line at 20 µg
Lane 3: HDFn Cell LIne at 20 µg
Lane 4: Jurkat Cell Line at 20 µg
Lane 5: HEK293T Cell Line at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 91 kDa
The 120 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Ctip1 / BCL-11A.
All lanes: Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (AB19487) at 1 µg/mL
All lanes: Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg
All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (AB97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 91 kDa
Observed band size: 120 kDa, 37 kDa, 42 kDa, 62 kDa
Exposure time: 20min
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