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AB264423

Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free

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Rabbit Recombinant Monoclonal Ctip1/BCL-11A antibody. Suitable for IP, WB, Flow Cyt and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human BCL11A aa 1-50.

View Alternative Names

CTIP1, EVI9, KIAA1809, ZNF856, BCL11A, BCL11 transcription factor A, B-cell CLL/lymphoma 11A, B-cell lymphoma/leukemia 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, Zinc finger protein 856, BCL-11A, EVI-9

4 Images
Flow Cytometry - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human Bcl11a with ab264423 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)
  • IP

Supplier Data

Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)

Ctip1/BCL-11A was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab264423 at 6 μl/mg lysate. Western blot was performed from the immunoprecipitate using ab264423 at 1/1000 dilution.

Lane 1 : ab264423 IP in Ramos whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 75 secs.

All lanes:

Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)

Predicted band size: 91 kDa

false

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)
  • WB

Supplier Data

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423) at 1/1000 dilution

Lane 1:

Ramos whole cell lysate at 50 µg

Lane 2:

HEK-293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

MOLT4 whole cell lysate at 50 µg

Lane 5:

U-2 OS whole cell lysate at 50 µg

Lane 6:

Raji whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 91 kDa

false

Exposure time: 3min

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)
  • WB

Supplier Data

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423) at 1/1000 dilution

Lane 1:

BW5147.3 whole cell lysate at 50 µg

Lane 2:

A20 whole cell lysate at 50 µg

Lane 3:

RenCa whole cell lysate at 50 µg

Lane 4:

EL4 whole cell lysate at 50 µg

Lane 5:

CH27 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 91 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR073G

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human BCL11A aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9H165

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  • 0
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Secondary Antibodies

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Primary Antibodies

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Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>6 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from TCS.
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor (PubMed : 16704730, PubMed : 29606353). Associated with the BAF SWI/SNF chromatin remodeling complex (PubMed : 23644491, PubMed : 39607926). Binds to the 5'-TGACCA-3' sequence motif in regulatory regions of target genes, including a distal promoter of the HBG1 hemoglobin subunit gamma-1 gene (PubMed : 29606353, PubMed : 39423807). Involved in regulation of the developmental switch from gamma- to beta-globin, probably via direct repression of HBG1; hence indirectly repressing fetal hemoglobin (HbF) level (PubMed : 26375765, PubMed : 29606353, PubMed : 39423807, PubMed : 39607926). Involved in brain development (PubMed : 27453576). May play a role in hematopoiesis (By similarity). Essential factor in lymphopoiesis required for B-cell formation in fetal liver (By similarity). May function as a modulator of the transcriptional repression activity of NR2F2 (By similarity).
See full target information BCL11A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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