Rabbit Recombinant Monoclonal Ctip1/BCL-11A antibody. Suitable for IP, WB, Flow Cyt and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human BCL11A aa 1-50.
Images
![Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-blr073g-bsa-free-ab264423--western-blot-img119868.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)")
![Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-blr073g-bsa-free-ab264423--immunoprecipitation-img54458.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)")
![Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-blr073g-bsa-free-ab264423--western-blot-img133654.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)")
![Flow Cytometry - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-blr073g-bsa-free-ab264423--flow-cytometry-img437675.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human BCL11A aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H165
Reactivity data
Select an application| IP | WB | Flow Cyt | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 6 μl/mg lysate |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µL for 106 Cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Transcription factor (PubMed:16704730, PubMed:29606353). Associated with the BAF SWI/SNF chromatin remodeling complex (PubMed:23644491). Binds to the 5'-TGACCA-3' sequence motif in regulatory regions of target genes, including a distal promoter of the HBG1 hemoglobin subunit gamma-1 gene (PubMed:29606353). Involved in regulation of the developmental switch from gamma- to beta-globin, probably via direct repression of HBG1; hence indirectly repressing fetal hemoglobin (HbF) level (PubMed:29606353, PubMed:26375765). Involved in brain development (PubMed:27453576). May play a role in hematopoiesis (By similarity). Essential factor in lymphopoiesis required for B-cell formation in fetal liver (By similarity). May function as a modulator of the transcriptional repression activity of NR2F2 (By similarity).
Alternative names
B-cell lymphoma/leukemia 11A, BCL-11A, B-cell CLL/lymphoma 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, Zinc finger protein 856, EVI-9, KIAA1809, ZNF856, EVI9, CTIP1, BCL11A
Recommended products
Rabbit Recombinant Monoclonal Ctip1/BCL-11A antibody. Suitable for IP, WB, Flow Cyt and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human BCL11A aa 1-50.
Alternative names
B-cell lymphoma/leukemia 11A, BCL-11A, B-cell CLL/lymphoma 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, Zinc finger protein 856, EVI-9, KIAA1809, ZNF856, EVI9, CTIP1, BCL11A
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human BCL11A aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H165
- Clone number
BLR073G
- Purification technique
Affinity purification
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is sold under License from Bethyl Laboratories, Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-blr073g-bsa-free-ab264423--western-blot-img119868.jpg "Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)")
Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)
Cells prepared using NETN lysis buffer.
All lanes: Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423) at 1/1000 dilution
Lane 1: Ramos whole cell lysate at 50 µg
Lane 2: HEK-293T whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: MOLT4 whole cell lysate at 50 µg
Lane 5: U-2 OS whole cell lysate at 50 µg
Lane 6: Raji whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 91 kDa
Exposure time: 3min
![Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-blr073g-bsa-free-ab264423--immunoprecipitation-img54458.jpg "Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)")
Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)
Ctip1/BCL-11A was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab264423 at 6 μl/mg lysate. Western blot was performed from the immunoprecipitate using ab264423 at 1/1000 dilution.
Lane 1: ab264423 IP in Ramos whole cell lysate.
Lane 2: Control IgG.Exposure time: 75 secs.
All lanes: Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)
Predicted band size: 91 kDa
![Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-blr073g-bsa-free-ab264423--western-blot-img133654.jpg "Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)")
Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)
Cells prepared using NETN lysis buffer.
All lanes: Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423) at 1/1000 dilution
Lane 1: BW5147.3 whole cell lysate at 50 µg
Lane 2: A20 whole cell lysate at 50 µg
Lane 3: RenCa whole cell lysate at 50 µg
Lane 4: EL4 whole cell lysate at 50 µg
Lane 5: CH27 whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 91 kDa
Exposure time: 3min
![Flow Cytometry - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423), expandable thumbnail](https://content.abcam.com/products/images/ctip1-bcl-11a-antibody-blr073g-bsa-free-ab264423--flow-cytometry-img437675.jpg "Flow Cytometry - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)")
Flow Cytometry - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)
Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human Bcl11a with ab264423 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: DyLight® 488-conjugated goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (DyLight® 488) ab96883).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com