AB233713

Anti-Ctip2 antibody [1F8G8]

Name: Anti-Ctip2 antibody [1F8G8] (ab233713) | Abcam
Brand: Abcam Limited
SKU: AB233713
Price: 520 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(1 Publication)

Mouse Monoclonal Ctip2 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BCL11B aa 1-150.

View Alternative Names

CTIP2, RIT1, BCL11B, B-cell lymphoma/leukemia 11B, BCL-11B, B-cell CLL/lymphoma 11B, COUP-TF-interacting protein 2, Radiation-induced tumor suppressor gene 1 protein, hRit1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [1F8G8] (AB233713)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [1F8G8] (AB233713)

Paraffin-embedded human stomach cancer tissue stained for Ctip2 using ab233713 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [1F8G8] (AB233713)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [1F8G8] (AB233713)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ctip2 (green) using ab233713 at 1/50 dilution in ICC/IF. Actin is stained with AlexaFluor^®555-Phalloidin (red). Blue : DRAQ5 DNA dye.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [1F8G8] (AB233713)

Paraffin-embedded human colon cancer tissue stained for Ctip2 using ab233713 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Flow Cytometry - Anti-Ctip2 antibody [1F8G8] (AB233713)

Flow Cyt

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Flow Cytometry - Anti-Ctip2 antibody [1F8G8] (AB233713)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Ctip2 with ab233713 at 1/200 dilution (green) compared with a negative control (red).

Flow Cyt

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Flow Cytometry - Anti-Ctip2 antibody [1F8G8] (AB233713)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Ctip2 with ab233713 at 1/200 dilution (green) compared with a negative control (red).

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Western blot - Anti-Ctip2 antibody [1F8G8] (AB233713)

All lanes:

Western blot - Anti-Ctip2 antibody [1F8G8] (ab233713) at 1/500 dilution

Lane 1:

Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

Ctip2 (aa1-150)-hIgG-Fc-transfected HEK-293 cell lysate

Predicted band size: 96 kDa

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Western blot - Anti-Ctip2 antibody [1F8G8] (AB233713)

All lanes:

Western blot - Anti-Ctip2 antibody [1F8G8] (ab233713) at 1/500 dilution

All lanes:

Recombinant human Ctip2 (aa1-150) protein

Predicted band size: 96 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1F8G8

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human BCL11B aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9C0K0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/250", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals. Essential in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of the receptors CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus (PubMed : 27959755). Is a regulator of IL2 promoter and enhances IL2 expression in activated CD4(+) T-lymphocytes (PubMed : 16809611). Tumor-suppressor that represses transcription through direct, TFCOUP2-independent binding to a GC-rich response element (By similarity). May also function in the P53-signaling pathway (By similarity).

See full target information BCL11B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 20:285 PubMed38037097

2023

Single-cell RNA sequencing unveils Lrg1's role in cerebral ischemia‒reperfusion injury by modulating various cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohui Ruan,Guosheng Cao,Yisong Qian,Longsheng Fu,Jinfang Hu,Tiantian Xu,Yaoqi Wu,Yanni Lv

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