Anti-Ctip2 antibody

• IP

Unknown

Immunoprecipitation - Anti-Ctip2 antibody (AB28448)

Ctip2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Ctip2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab28448.
Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Bands : 125, 130kDa : Ctip2.

All lanes:

Immunoprecipitation - Anti-Ctip2 antibody (ab28448)

Predicted band size: 96 kDa

false

• WB

Project

Western blot - Anti-Ctip2 antibody (AB28448)

All lanes:

Western blot - Anti-Ctip2 antibody (ab28448) at 1 µg/mL

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat nuclear extract lysate (<a href='/en-us/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 96 kDa

Observed band size: 125 kDa,130 kDa

false