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Rabbit Recombinant Monoclonal Ctip2 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.


Images

Immunoprecipitation - Anti-Ctip2 antibody [EPR23120-25] (AB240636), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (AB240636), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [EPR23120-25] (AB240636), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Ctip2 antibody [EPR23120-25] (AB240636), expandable thumbnail
  • Western blot - Anti-Ctip2 antibody [EPR23120-25] (AB240636), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Expected
Rat
Tested
Expected
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals. Essential in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of the receptors CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus (PubMed:27959755). Is a regulator of IL2 promoter and enhances IL2 expression in activated CD4(+) T-lymphocytes (PubMed:16809611). Tumor-suppressor that represses transcription through direct, TFCOUP2-independent binding to a GC-rich response element (By similarity). May also function in the P53-signaling pathway (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Ctip2 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23120-25

Purification technique

Affinity purification Protein A

Specificity

Please note the species reactivity is application dependent.

WB: Human, mouse and rat.

ICC/IF: Human.

IHC-P: Human, mouse and rat.

FC: Human.

IP: Human.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

  • Immunoprecipitation - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunoprecipitation - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Ctip2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab240636 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240636 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Jurkat whole cell lysate 10μg

    Lane 2: ab240636 IP in Jurkat whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab240636 in Jurkat whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Predicted band size: 96 kDa

    Observed band size: 120 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on rat spleen (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte) and Daudi (human Burkitt's lymphoma lymphoblast) cells labeling Ctip2 with ab240636 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in Jurkat cells.

    Negative control: Daudi (PMID:23383087).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Daudi (human Burkitt's lymphoma lymphoblast, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labeling Ctip2 with ab240636 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Daudi (PMID:23383087).

  • Western blot - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Western blot - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23383087, 24407555).

    All lanes: Western blot - Anti-Ctip2 antibody [EPR23120-25] (ab240636) at 1/1000 dilution

    Lane 1: Mouse hippocampus tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Rat hippocampus tissue lysate at 20 µg

    Lane 4: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 96 kDa

    Observed band size: 120 kDa

  • Western blot - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Western blot - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 5.5 seconds.

    Negative control: Daudi (PMID: 23383087).

    Fresh lysates are recommended.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23383087, 24407555).

    All lanes: Western blot - Anti-Ctip2 antibody [EPR23120-25] (ab240636) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Lane 2: Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 96 kDa

    Observed band size: 120 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on rat hippocampus (PMID: 19955470, PMID: 25757017) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on mouse hippocampus (PMID: 19955470, PMID: 25757017) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on mouse spleen (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on human spleen (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on human tonsil (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded human hippocampus* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab240636, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Negative control image: IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded mouse cerebellum performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab240636, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    Negative control image: IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded human cerebellum* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab240636, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

    IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded mouse hippocampus performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab240636, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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