Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

Rabbit Recombinant Monoclonal Ctip2 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (AB269367), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Tested	Expected	Expected
Rat	Tested	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information BCL11B

Function

Key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals. Essential in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of the receptors CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus (PubMed:27959755). Is a regulator of IL2 promoter and enhances IL2 expression in activated CD4(+) T-lymphocytes (PubMed:16809611). Tumor-suppressor that represses transcription through direct, TFCOUP2-independent binding to a GC-rich response element (By similarity). May also function in the P53-signaling pathway (By similarity).

Alternative names

CTIP2, RIT1, BCL11B, B-cell lymphoma/leukemia 11B, BCL-11B, B-cell CLL/lymphoma 11B, COUP-TF-interacting protein 2, Radiation-induced tumor suppressor gene 1 protein, hRit1

Recommended products

Rabbit Recombinant Monoclonal Ctip2 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Carrier free

Yes

Clone number

EPR23120-25

Purification technique

Affinity purification Protein A

Specificity

Please note the species reactivity is application dependent.

WB: Human, mouse and rat.

ICC/IF: Human.

IHC-P: Human, mouse and rat.

FC: Human.

IP: Human.

Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab269367 is the carrier-free version of Anti-Ctip2 antibody [EPR23120-25] ab240636.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Immunoprecipitation - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
Ctip2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with Anti-Ctip2 antibody [EPR23120-25] ab240636 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Ctip2 antibody [EPR23120-25] ab240636 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat whole cell lysate 10μg
Lane 2: Anti-Ctip2 antibody [EPR23120-25] ab240636 IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Ctip2 antibody [EPR23120-25] ab240636 in Jurkat whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
All lanes: Immunoprecipitation - Anti-Ctip2 antibody [EPR23120-25] (Anti-Ctip2 antibody [EPR23120-25] ab240636)
Predicted band size: 96 kDa
Observed band size: 120 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Ctip2 with Anti-Ctip2 antibody [EPR23120-25] ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on rat spleen (PMID: 30089823) is observed. The section was incubated with Anti-Ctip2 antibody [EPR23120-25] ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte) and Daudi (human Burkitt's lymphoma lymphoblast) cells labeling Ctip2 with Anti-Ctip2 antibody [EPR23120-25] ab240636 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in Jurkat cells.
Negative control: Daudi (PMID:23383087).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
Flow Cytometry (Intracellular) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Daudi (human Burkitt's lymphoma lymphoblast, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labeling Ctip2 with Anti-Ctip2 antibody [EPR23120-25] ab240636 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: Daudi (PMID:23383087).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Ctip2 with Anti-Ctip2 antibody [EPR23120-25] ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on rat hippocampus (PMID: 19955470, PMID: 25757017) is observed. The section was incubated with Anti-Ctip2 antibody [EPR23120-25] ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Ctip2 with Anti-Ctip2 antibody [EPR23120-25] ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on mouse hippocampus (PMID: 19955470, PMID: 25757017) is observed. The section was incubated with Anti-Ctip2 antibody [EPR23120-25] ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ctip2 with Anti-Ctip2 antibody [EPR23120-25] ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on mouse spleen (PMID: 30089823) is observed. The section was incubated with Anti-Ctip2 antibody [EPR23120-25] ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Ctip2 with Anti-Ctip2 antibody [EPR23120-25] ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on human spleen (PMID: 30089823) is observed. The section was incubated with Anti-Ctip2 antibody [EPR23120-25] ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Ctip2 with Anti-Ctip2 antibody [EPR23120-25] ab240636 at 1/500 dilution (0.96 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on human tonsil (PMID: 30089823) is observed. The section was incubated with Anti-Ctip2 antibody [EPR23120-25] ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded mouse hippocampus performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with Anti-Ctip2 antibody [EPR23120-25] ab240636, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded human hippocampus* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with Anti-Ctip2 antibody [EPR23120-25] ab240636, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
Negative control image: IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded mouse cerebellum performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with Anti-Ctip2 antibody [EPR23120-25] ab240636, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
Negative control image: IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded human cerebellum* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with Anti-Ctip2 antibody [EPR23120-25] ab240636, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ctip2 antibody [EPR23120-25] ab240636).