Anti-CTNNA1 antibody [1G5]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody [1G5] (AB231306)

ab231306 staining CTNNA1 in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab231306 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• WB

Lab

Western blot - Anti-CTNNA1 antibody [1G5] (AB231306)

This data was developed using the same antibody clone in a different buffer formulation (ab231306).

ab231306 was shown to specifically react with CTNNA1 (α-catenin) in wild type HAP1 cells. No band was observed when CTNNA1 (α-catenin) knockout samples were used. Wild-type and CTNNA1 (α-catenin) knockout samples were subjected to SDS-PAGE. ab231306 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentraion and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CTNNA1 antibody [1G5] (ab231306)

Lane 1:

HAP1 whole cell lysate at 20 µg

Lane 2:

HAP1 CTNNA1 KO whole cell lysate at 20 µg

Lane 3:

Rat colon whole tissue lysate at 20 µg

Predicted band size: 100 kDa

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