AB227181

Anti-CTNNA1 antibody

Name: Anti-CTNNA1 antibody (ab227181) | Abcam
Brand: Abcam Limited
SKU: AB227181
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal CTNNA1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Dog, Cat, Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Catenin alpha-1.

View Alternative Names

Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, CTNNA1

9 Images

Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody (AB227181)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody (AB227181)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CTNNA1 (green) using ab227181 at 1/500 dilution in ICC/IF.

Counterstain : alpha Tubulin was stained by an alpha Tubulin antibody at 1/500 dilution (red). Nuclear Counterstain : Hoechst 33342 (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody (AB227181)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling alpha 1 Catenin protein at cell junction using ab227181 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue). Scale bar = 10 µm

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody (AB227181)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody (AB227181)

Paraffin-embedded mouse intestine tissue stained for CTNNA1 using ab227181 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody (AB227181)

Paraffin-embedded mouse prostate carcinoma tissue stained for CTNNA1 using ab227181 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-CTNNA1 antibody (AB227181)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-CTNNA1 antibody (ab227181) at 1/10000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 30 µg

Lane 2:

JC whole cell lysate at 30 µg

Predicted band size: 100 kDa

false

Supplier Data

Western blot - Anti-CTNNA1 antibody (AB227181)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-CTNNA1 antibody (ab227181) at 1/10000 dilution

All lanes:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

Predicted band size: 100 kDa

false

Supplier Data

Western blot - Anti-CTNNA1 antibody (AB227181)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-CTNNA1 antibody (ab227181) at 1/1000 dilution

All lanes:

MDCK Whole cell extract at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 100 kDa

false

Supplier Data

Western blot - Anti-CTNNA1 antibody (AB227181)

All lanes:

Western blot - Anti-CTNNA1 antibody (ab227181) at 1/1000 dilution

Lane 1:

293T cell lysate at 30 µg

Lane 2:

A431 cell lysate at 30 µg

Lane 3:

HeLa cell lysate at 30 µg

Lane 4:

HepG2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 100 kDa

false

Supplier Data

Western blot - Anti-CTNNA1 antibody (AB227181)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-CTNNA1 antibody (ab227181) at 1/1000 dilution

All lanes:

PG 4 Whole cell extract at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Dog, Cat

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Catenin alpha-1. The exact immunogen used to generate this antibody is proprietary information.

P35221

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTNNA1 also known as alpha-Catenin is an important protein that is involved in cell adhesion. With a molecular weight of about 100 kDa CTNNA1 functions mechanically by linking cadherins which are transmembrane proteins to the actin cytoskeleton in cells. This linkage strengthens cellular junctions and maintains the integrity of tissue architecture. CTNNA1 is widely expressed in epithelial tissues where strong cell-cell adhesion is important for maintaining tissue structure.

Biological function summary

CTNNA1 is important for maintaining cellular integrity and function. It is a part of the adherens junction complex which is critical for the creation of stable cell-cell junctions in tissues. Through the adherens junction it directly associates with E-cadherin and indirectly interacts with the actin cytoskeleton. This structural configuration allows epithelial cells to remain tightly connected facilitating signal transmission between cells for coordinated tissue functioning.

Pathways

CTNNA1 plays a significant role in the Wnt signaling pathway and the regulation of actin dynamics. In the Wnt pathway CTNNA1 is associated with cell fate determination and is known to interact with beta-catenin an important component of this signaling cascade. Additionally by influencing actin cytoskeleton organization CTNNA1 affects cell movement and signal transduction essential for processes like embryogenesis and wound healing.

Disruptions in CTNNA1 function have been linked to cancer and cardiomyopathy. The loss or mutation of CTNNA1 can lead to diminished cell adhesion promoting cancer cell invasion and metastasis often seen in gastric and breast cancers. Moreover its interaction with genes like KIF7 influences cardiomyopathy where structural cardiac tissue changes impair heart function. Understanding CTNNA1's role in these conditions can provide insight for potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). May play a crucial role in cell differentiation.

See full target information Catenin alpha-1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw6926 PubMed40845109

2025

Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Yu,Mincheng Yu,Peiyi Xie,Lei Guo,Yufei Zhao,Wenxin Xu,Xian Li,Mengyuan Wu,Zihao Zhang,Zheng Chen,Yongsheng Xiao,Jian Zhou,Jia Fan,Mien-Chie Hung,Yongfeng Xu,Bo Zhang,Qinghai Ye,Hui Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CTNNA1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody (AB227181)

Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody (AB227181)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody (AB227181)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody (AB227181)

Western blot - Anti-CTNNA1 antibody (AB227181)

Western blot - Anti-CTNNA1 antibody (AB227181)

Western blot - Anti-CTNNA1 antibody (AB227181)

Western blot - Anti-CTNNA1 antibody (AB227181)

Western blot - Anti-CTNNA1 antibody (AB227181)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise