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Rabbit Recombinant Monoclonal CTNNA1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

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Images

Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (AB226010), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (AB226010), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (AB226010), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (AB226010), expandable thumbnail
  • Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (AB226010), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Tested
Not recommended
Tested
Not recommended
Mouse
Tested
Expected
Not recommended
Tested
Not recommended
Rat
Tested
Expected
Not recommended
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

1 product for Alternative Version

Target data

Function

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). May play a crucial role in cell differentiation.

Alternative names

Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, CTNNA1

Recommended products

Rabbit Recombinant Monoclonal CTNNA1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EP1793Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab226010 is the carrier-free version of Anti-CTNNA1 antibody [EP1793Y] ab51032.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CTNNA1 also known as alpha-Catenin is an important protein that is involved in cell adhesion. With a molecular weight of about 100 kDa CTNNA1 functions mechanically by linking cadherins which are transmembrane proteins to the actin cytoskeleton in cells. This linkage strengthens cellular junctions and maintains the integrity of tissue architecture. CTNNA1 is widely expressed in epithelial tissues where strong cell-cell adhesion is important for maintaining tissue structure.

Biological function summary

CTNNA1 is important for maintaining cellular integrity and function. It is a part of the adherens junction complex which is critical for the creation of stable cell-cell junctions in tissues. Through the adherens junction it directly associates with E-cadherin and indirectly interacts with the actin cytoskeleton. This structural configuration allows epithelial cells to remain tightly connected facilitating signal transmission between cells for coordinated tissue functioning.

Pathways

CTNNA1 plays a significant role in the Wnt signaling pathway and the regulation of actin dynamics. In the Wnt pathway CTNNA1 is associated with cell fate determination and is known to interact with beta-catenin an important component of this signaling cascade. Additionally by influencing actin cytoskeleton organization CTNNA1 affects cell movement and signal transduction essential for processes like embryogenesis and wound healing.

Associated diseases and disorders

Disruptions in CTNNA1 function have been linked to cancer and cardiomyopathy. The loss or mutation of CTNNA1 can lead to diminished cell adhesion promoting cancer cell invasion and metastasis often seen in gastric and breast cancers. Moreover its interaction with genes like KIF7 influences cardiomyopathy where structural cardiac tissue changes impair heart function. Understanding CTNNA1's role in these conditions can provide insight for potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010), expandable thumbnail

    Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

    Anti-GCLC antibody [EP13475] ab190685 at 1/100 dilution immunoprecipitating CTNNA1 in Jurkat HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CTNNA1 antibody [EP1793Y] ab51032 in HeLa whole cell lysate
    For western blotting, Anti-CTNNA1 antibody [EP1793Y] ab51032 at 1/500 dilution and VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution were used.

    Blocking and diluting buffer: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTNNA1 antibody [EP1793Y] ab51032).

    All lanes: Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] (Anti-CTNNA1 antibody [EP1793Y] ab51032)

    Predicted band size: 100 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

    Paraffin-embedded rat stomach tissue stained for CTNNA1 with Anti-CTNNA1 antibody [EP1793Y] ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.

    Positive staining was seen on rat stomach.

    The section was incubated with Anti-CTNNA1 antibody [EP1793Y] ab51032 for 30 minutes at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTNNA1 antibody [EP1793Y] ab51032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

    Paraffin-embedded mouse liver tissue stained for CTNNA1 with Anti-CTNNA1 antibody [EP1793Y] ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.

    Positive staining was seen on mouse liver.

    The section was incubated with Anti-CTNNA1 antibody [EP1793Y] ab51032 for 30 minutes at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTNNA1 antibody [EP1793Y] ab51032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

    Paraffin-embedded human stomach tissue stained for CTNNA1 with Anti-CTNNA1 antibody [EP1793Y] ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.

    Positive staining was seen on human stomach.

    The section was incubated with Anti-CTNNA1 antibody [EP1793Y] ab51032 for 30 minutes at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTNNA1 antibody [EP1793Y] ab51032).

  • Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010), expandable thumbnail

    Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

    Expsure times

    Lane 1-4: 180 seconds

    Lane 5,7: 40 seconds

    Lane 6: 5 seconds

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CTNNA1 antibody [EP1793Y] ab51032).

    All lanes: Western blot - Anti-CTNNA1 antibody [EP1793Y] (Anti-CTNNA1 antibody [EP1793Y] ab51032) at 1/10000 dilution

    Lane 1: Mouse heart lysate at 20 µg

    Lane 2: Mouse kidney lysate at 20 µg

    Lane 3: Rat brain lysate at 20 µg

    Lane 4: Rat kidney lysate at 20 µg

    Lane 5: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 7: HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 100 kDa

    Observed band size: 100 kDa

  • Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010), expandable thumbnail

    Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

    This WB data was generated using the same anti-CTNNA1 antibody clone [EP1793Y] in a different buffer formulation (cat# Anti-CTNNA1 antibody [EP1793Y] ab51032).

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: CTNNA1 HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: HEK293 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-CTNNA1 antibody [EP1793Y] ab51032 observed at 100 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    Anti-CTNNA1 antibody [EP1793Y] ab51032 was shown to recognize CTNNA1 in wild-type cells as signal was lost at the expected MW in CTNNA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CTNNA1 knockout samples were subjected to SDS-PAGE. Anti-CTNNA1 antibody [EP1793Y] ab51032 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CTNNA1 antibody [EP1793Y] (Anti-CTNNA1 antibody [EP1793Y] ab51032)

    Predicted band size: 100 kDa

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