Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)

Rabbit Recombinant Monoclonal CTNNA3 antibody. Carrier free. Suitable for mIHC, IP, WB, IHC-P and reacts with Mouse, Human, Rat, Dog samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (AB250780), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	IP	WB	IHC-P
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Tested	Tested
Rat	Expected	Expected	Tested	Tested
Dog	Expected	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -
Species Human	Dilution info -	Notes Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Dog	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Dog	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information Catenin alpha-3

Function

May be involved in formation of stretch-resistant cell-cell adhesion complexes.

Alternative names

Catenin alpha-3, Alpha T-catenin, Cadherin-associated protein, CTNNA3

Recommended products

Rabbit Recombinant Monoclonal CTNNA3 antibody. Carrier free. Suitable for mIHC, IP, WB, IHC-P and reacts with Mouse, Human, Rat, Dog samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR18307
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab250780 is the carrier-free version of Anti-CTNNA3 antibody [EPR18307] ab184916.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CTNNA3 also known as alpha-T-catenin is a protein that plays a significant role in cellular adhesion. Alpha-T-catenin belongs to the catenin family and has a mass of approximately 100 kDa. It interacts with the actin cytoskeleton by binding to beta-catenin at adherens junctions which are important for maintaining cell structures. The expression of CTNNA3 occurs mainly in cardiac and muscle tissues an indicator of its specialized function in the structure and function of these cell types.

Biological function summary

The protein functions as an important component in cellular adhesion linking cadherins to the actin cytoskeleton which impacts tissue stability and integrity. CTNNA3 forms a complex with cadherin-catenin a structure vital for maintaining strong cell-cell adhesion. The protein ensures that cells within cardiac and skeletal muscles maintain their structural coherence during mechanical stress playing an integral role in muscle function and structural integrity.

Pathways

Alpha-T-catenin contributes to the regulation of Wnt signaling and adherens junctions assembly. These pathways involve cell cycle regulation cellular adhesion and migration. Within these pathways CTNNA3 interacts with other proteins like beta-catenin and cadherins coordinating cell behavior and maintenance processes. The protein's activity in the Wnt signaling pathway helps manipulate gene expression which ultimately affects cellular proliferation and differentiation.

Associated diseases and disorders

Aberrations in CTNNA3 are linked to specific cardiac and muscle conditions such as arrhythmogenic right ventricular cardiomyopathy (ARVC) and muscular dystrophies. Disrupted function or expression of alpha-T-catenin can lead to compromised cardiac cell adhesion contributing to the development of ARVC. Furthermore mutations or downregulations of CTNNA3 are associated with alterations in beta-catenin interaction leading to impaired cellular functions contributing to disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
This data was developed using Anti-CTNNA3 antibody [EPR18307] ab184916, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CTNNA3 antibody [EPR18307] (Anti-CTNNA3 antibody [EPR18307] ab184916) at 1/50000 dilution
Lane 1: HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg
Lane 2: C2C12 (Mouse myoblast cell line) whole cell lysate at 20 µg
Lane 3: Rat1 (Rat fibroblast cell line) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
This data was developed using Anti-CTNNA3 antibody [EPR18307] ab184916, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling CTNNA3 with Anti-CTNNA3 antibody [EPR18307] ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on tumor cells of Human colonic adenocarcinoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
This data was developed using Anti-CTNNA3 antibody [EPR18307] ab184916, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CTNNA3 with Anti-CTNNA3 antibody [EPR18307] ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Mouse cardiac muscle is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
This data was developed using Anti-CTNNA3 antibody [EPR18307] ab184916, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CTNNA3 antibody [EPR18307] (Anti-CTNNA3 antibody [EPR18307] ab184916) at 1/5000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Human fetal heart lysate at 10 µg
Lane 3: Human fetal kidney lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
This data was developed using Anti-CTNNA3 antibody [EPR18307] ab184916, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling CTNNA3 with Anti-CTNNA3 antibody [EPR18307] ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining of normal Human cardiac muscle is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
This data was developed using Anti-CTNNA3 antibody [EPR18307] ab184916, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CTNNA3 antibody [EPR18307] (Anti-CTNNA3 antibody [EPR18307] ab184916) at 1/5000 dilution
Lane 1: Mouse brain tissue lysate at 10 µg
Lane 2: Mouse heart tissue lysate at 10 µg
Lane 3: Rat brain tissue lysate at 10 µg
Lane 4: Rat heart tissue lysate at 10 µg
Lane 5: C6 (Rat glial tumor cells) whole cell lysate at 10 µg
Lane 6: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg
Lane 7: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 1s
Immunoprecipitation - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
This data was developed using Anti-CTNNA3 antibody [EPR18307] ab184916, the same antibody clone in a different buffer formulation.
CTNNA3 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with Anti-CTNNA3 antibody [EPR18307] ab184916 at 1/100 dilution. Western blot was performed from the immunoprecipitate using Anti-CTNNA3 antibody [EPR18307] ab184916 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK-293 whole cell lysate, 10 μg (Input).
Lane 2: Anti-CTNNA3 antibody [EPR18307] ab184916 IP in HEK-293 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CTNNA3 antibody [EPR18307] ab184916 in HEK-293 whole cell lysate.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 1 second.
All lanes: Immunoprecipitation - Anti-CTNNA3 antibody [EPR18307] (Anti-CTNNA3 antibody [EPR18307] ab184916)
Predicted band size: 100 kDa
Exposure time: 1s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
This data was developed using Anti-CTNNA3 antibody [EPR18307] ab184916, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling CTNNA3 with Anti-CTNNA3 antibody [EPR18307] ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Rat cardiac muscle is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
This data was developed using Anti-CTNNA3 antibody [EPR18307] ab184916, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CTNNA3 antibody [EPR18307] (Anti-CTNNA3 antibody [EPR18307] ab184916) at 1/50000 dilution
All lanes: MDCK (Canine kidney cell line) whole cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 1s
Multiplex immunohistochemistry - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
This data was developed using Anti-CTNNA3 antibody [EPR18307] ab184916, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle labelling Cardiac Troponin T with Anti-Cardiac Troponin T antibody [EPR26703-8] ab307152 at 1/5000 dilution (0.102 µg/mL) (B), CTNNA3 with Anti-CTNNA3 antibody [EPR18307] ab184916 at 1/2000 dilution (0.942 μg/ml) (C) and PDystrophin with Anti-Dystrophin antibody [EPR23336-129] ab275391 at 1/500 dilution (2.08 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A: merged staining of anti-Dystrophin (red; Opal™690), anti-Cardiac troponin T (green; Opal™520) and anti-CTNNA3 (gray; Opal™570) on human cardiac muscle.
Panel B: anti-Cardiac troponin T stained on cytoplasm of cardiac muscle.
Panel C: anti-CTNNA3 stained on intercalated discs.
Panel D: anti-Dystrophin stained on membrane of cardiac muscle.

The section was incubated in three rounds of staining: in the order of Anti-Dystrophin antibody [EPR23336-129] ab275391, Anti-Cardiac Troponin T antibody [EPR26703-8] ab307152, and Anti-CTNNA3 antibody [EPR18307] ab184916 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
This data was developed using Anti-CTNNA3 antibody [EPR18307] ab184916, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cardiac muscle tissue staining Titin with Anti-Titin antibody [EPR26167-75] ab307446 at a 1/1000 ( 0.475 µg/ml) dilution, CTNNA3 with Anti-CTNNA3 antibody [EPR18307] ab184916 at 1/2000 ( 0.942 µg/ml) dilution and Dystrophin with Anti-Dystrophin antibody [EPR21189] ab218198 at 1/5000 ( 0.110 µg/ml) dilution.
Panel A: merged staining of anti-Titin (gray; Opal™570), anti-CTNNA3 (green; Opal™520) and anti-Dystrophin (magenta; Opal™690) on mouse cardiac muscle.
Panel B: anti-Titin staining nucleus and cytoplasm in mouse cardiac muscle.
Panel C: anti-CTNNA3 staining intercalated discs in mouse cardiac muscle.
Panel D: anti-Dystrophin staining membrane in mouse cardiac muscle.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Titin antibody [EPR26167-75] ab307446, Anti-CTNNA3 antibody [EPR18307] ab184916 and Anti-Dystrophin antibody [EPR21189] ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^° RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.