Anti-CTNNBL1 antibody [EP2669Y] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CTNNBL1 antibody [EP2669Y] - BSA and Azide free (AB284752)

This data was developed using ab76243, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of CTNNBL1 in HeLa cells using ab76243 at a 1/100 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNBL1 antibody [EP2669Y] - BSA and Azide free (AB284752)

This data was developed using ab76243, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of human breast tissue staining CTNNBL1 with ab76243 at 1/100 dilution. The human breast sections were performed by LeicaDS9800 (Bond™ Polymer Refine Detection). The sections were using Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, then labeling ab76243 at 1/100 dilution for 30 mins at room temperature. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• Flow Cyt

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Flow Cytometry - Anti-CTNNBL1 antibody [EP2669Y] - BSA and Azide free (AB284752)

This data was developed using ab76243, the same antibody clone in a different buffer formulation. Flow cytometry analysis showing positive staining in HeLa cells using ab76243 at 1 : 20 dilution, 5 µg/mL. Cells were fixed with 100μl 4% PFA for 10 min at RT, then permeabilized with 100μl 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab76243 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) for 30 min at room temperature.

• ICC

Supplier Data

Immunocytochemistry - Anti-CTNNBL1 antibody [EP2669Y] - BSA and Azide free (AB284752)

This data was developed using ab76243, the same antibody clone in a different buffer formulation. HeLa cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab76243 at 1 : 50 was incubated overnight at 4° C, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) at 1/200 dilution, was co-incubated with ab76243 overnight at 4° C. Nucleus were visualized using DAPI.

• WB

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Western blot - Anti-CTNNBL1 antibody [EP2669Y] - BSA and Azide free (AB284752)

This data was developed using ab76243, the same antibody clone in a different buffer formulation.   Blocking and diluting buffer and concentration : 5% NFDM/TBST, Primary antibody : incubated for 1h at room temperature. Secondary antibody : incubated for 1h at 1 : 20,000 at room temperature (ab97051)

All lanes:

Western blot - Anti-CTNNBL1 antibody [EP2669Y] (<a href='/en-us/products/primary-antibodies/ctnnbl1-antibody-ep2669y-ab76243'>ab76243</a>) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole lysate at 15 µg

Lane 2:

MCF7 (Human breast adenocarcinoma epithelial cell) whole lysate at 15 µg

false

Exposure time: 180s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNBL1 antibody [EP2669Y] - BSA and Azide free (AB284752)

This data was developed using ab76243, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of CTNNBL1 in paraffin embedded human mammary gland using ab76243 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-CTNNBL1 antibody [EP2669Y] - BSA and Azide free (AB284752)

This data was developed using ab76243, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CTNNBL1 antibody [EP2669Y] - BSA and Azide free (ab284752) at 1/2000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 65 kDa

Observed band size: 65 kDa

false