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AB235000

Anti-CTP synthase/CTPS antibody

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(1 Publication)

Rabbit Polyclonal CTP synthase/CTPS antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CTPS1 aa 50-400.

View Alternative Names

CTPS, CTPS1, CTP synthase 1, CTP synthetase 1, UTP--ammonia ligase 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTP synthase/CTPS antibody (AB235000)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTP synthase/CTPS antibody (AB235000)

Paraffin-embedded human breast cancer tissue stained for CTP synthase/CTPS using ab235000 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTP synthase/CTPS antibody (AB235000)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTP synthase/CTPS antibody (AB235000)

Paraffin-embedded human pancreatic tissue stained for CTP synthase/CTPS using ab235000 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-CTP synthase/CTPS antibody (AB235000)
  • WB

Supplier Data

Western blot - Anti-CTP synthase/CTPS antibody (AB235000)

All lanes:

Western blot - Anti-CTP synthase/CTPS antibody (ab235000) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit at 1/10000 dilution

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CTPS1 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

P17812

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTP synthase also known as CTPS or the CTP protein is an enzyme involved in pyrimidine metabolism. It catalyzes the conversion of UTP to CTP an essential step in nucleotide biosynthesis. The enzyme exists in two isoforms CTPS1 and CTPS2 with both found in various tissues but CTPS2 has a higher expression in the brain. CTP synthase has a molecular mass of roughly 70 kDa. CTPS can assemble into filamentous structures under certain conditions influencing its enzymatic activity. These filaments sometimes referred to as cytoophidia are observed in many types of cells.
Biological function summary

CTP synthase plays a significant role in the regulation of nucleotide pools. The enzyme ensures the balance of CTP levels necessary for DNA RNA and phospholipid synthesis acting as a rate-limiting step in these processes. CTPS is part of larger enzymatic complexes that aid in cellular response to nutrient availability and DNA damage. Allosteric regulation and product feedback inhibition modulate CTP synthase activity maintaining cellular homeostasis and a proper supply of CTP for various biosynthetic pathways.

Pathways

CTP synthase is an important component in the pyrimidine biosynthesis pathway essential for nucleic acid and lipid metabolism. The enzyme's activity is tightly linked to the salvage pathways collaborating with enzymes like ribonucleotide reductase to regulate deoxyribonucleotide levels for DNA replication and repair. These interactions highlight the enzyme's involvement in cellular proliferation and response to metabolic stresses underpinning its regulatory function in metabolic pathways.

CTP synthase activity disorders relate to cancer and neurodegeneration. Dysregulation of CTPS is associated with increased proliferation seen in certain cancers given its role in nucleotide biosynthesis. Research also indicates a link between CTPS activity and neurodegenerative diseases as nucleotide imbalance affects neural function. Additionally interactions with proteins involved in cell cycle control and apoptosis such as p53 and Rb further highlight CTPS's role in disease etiology influencing therapeutic strategies targeting these diverse conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity.
See full target information CTPS1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29643 PubMed40804093

2025

miR-221 activates Sox11 to reduce brain injury after intracerebral hemorrhage via inhibiting neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Liang,Yongchun Liu,Renyang Liu,Xianmei Shen,Mingxia Xu
View all publications
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