AB244492

Anti-CTP synthase/CTPS antibody

Name: Anti-CTP synthase/CTPS antibody (ab244492) | Abcam
Brand: Abcam Limited
SKU: AB244492

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(3 Publications)

Rabbit Polyclonal CTP synthase/CTPS antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CTPS1 aa 500 to C-terminus.

View Alternative Names

CTPS, CTPS1, CTP synthase 1, CTP synthetase 1, UTP--ammonia ligase 1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CTP synthase/CTPS antibody (AB244492)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CTP synthase/CTPS antibody (AB244492)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for CTP synthase/CTPS (green) using ab244492 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTP synthase/CTPS antibody (AB244492)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTP synthase/CTPS antibody (AB244492)

Paraffin-embedded human skeletal muscle tissue stained for CTP synthase/CTPS using ab244492 at 1/200 dilution in immunohistochemical analysis. Low expression, as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTP synthase/CTPS antibody (AB244492)

Paraffin-embedded human smooth muscle tissue stained for CTP synthase/CTPS using ab244492 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CTPS1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P17812

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTP synthase also known as CTPS or the CTP protein is an enzyme involved in pyrimidine metabolism. It catalyzes the conversion of UTP to CTP an essential step in nucleotide biosynthesis. The enzyme exists in two isoforms CTPS1 and CTPS2 with both found in various tissues but CTPS2 has a higher expression in the brain. CTP synthase has a molecular mass of roughly 70 kDa. CTPS can assemble into filamentous structures under certain conditions influencing its enzymatic activity. These filaments sometimes referred to as cytoophidia are observed in many types of cells.

Biological function summary

CTP synthase plays a significant role in the regulation of nucleotide pools. The enzyme ensures the balance of CTP levels necessary for DNA RNA and phospholipid synthesis acting as a rate-limiting step in these processes. CTPS is part of larger enzymatic complexes that aid in cellular response to nutrient availability and DNA damage. Allosteric regulation and product feedback inhibition modulate CTP synthase activity maintaining cellular homeostasis and a proper supply of CTP for various biosynthetic pathways.

Pathways

CTP synthase is an important component in the pyrimidine biosynthesis pathway essential for nucleic acid and lipid metabolism. The enzyme's activity is tightly linked to the salvage pathways collaborating with enzymes like ribonucleotide reductase to regulate deoxyribonucleotide levels for DNA replication and repair. These interactions highlight the enzyme's involvement in cellular proliferation and response to metabolic stresses underpinning its regulatory function in metabolic pathways.

CTP synthase activity disorders relate to cancer and neurodegeneration. Dysregulation of CTPS is associated with increased proliferation seen in certain cancers given its role in nucleotide biosynthesis. Research also indicates a link between CTPS activity and neurodegenerative diseases as nucleotide imbalance affects neural function. Additionally interactions with proteins involved in cell cycle control and apoptosis such as p53 and Rb further highlight CTPS's role in disease etiology influencing therapeutic strategies targeting these diverse conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity.

See full target information CTPS1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 20:17 PubMed34991621

2022

CTPS1 promotes malignant progression of triple-negative breast cancer with transcriptional activation by YBX1.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiang Lin,Jie Zhang,Yan Li,Wenhui Guo,Lili Chen,Minyan Chen,Xiaobin Chen,Wenzhe Zhang,Xuan Jin,Meichen Jiang,Han Xiao,Chuan Wang,Chuangui Song,Fangmeng Fu

Journal of neuropathology and experimental neurology 80:944-954 PubMed34498062

2021

Nuclear IMPDH Filaments in Human Gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Narges Ahangari,David G Munoz,Josee Coulombe,Douglas A Gray,Elizabeth C Engle,Long Cheng,John Woulfe

Journal of translational medicine 19:344 PubMed34376200

2021

Heparin-binding growth factor (HDGF) drives radioresistance in breast cancer by activating the STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyun Qiu,Yan Ma,Xiaohua Chen,Liheng Zhou,Haibo Zhang,Guansheng Zhong,Lei Zhang,Jianming Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CTP synthase/CTPS antibody

Immunocytochemistry/ Immunofluorescence - Anti-CTP synthase/CTPS antibody (AB244492)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTP synthase/CTPS antibody (AB244492)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTP synthase/CTPS antibody (AB244492)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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