AB133743

Anti-CTP synthase/CTPS antibody [EPR8086(B)]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal CTP synthase/CTPS antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CTPS, CTPS1, CTP synthase 1, CTP synthetase 1, Protein-asparagine deamidase CTPS1, UTP--ammonia ligase 1

3 Images

Flow Cytometry (Intracellular) - Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB133743)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB133743)

Intracellular Flow Cytometry analysis of permeabilized Raji cells labelling CTP synthase/CTPS with ab133743 at 1/10 (red), with rabbit IgG negative control (green).

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Western blot - Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB133743)

All lanes:

Western blot - Anti-CTP synthase/CTPS antibody [EPR8086(B)] (ab133743) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

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OI-RD Scanning - Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB133743)

OI-RD Scanning

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OI-RD Scanning - Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB133743)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-CTP synthase/CTPS antibody [EPR8086(B)] - BSA and Azide free
578 PE

PE Anti-CTP synthase/CTPS antibody [EPR8086(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8086(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTP synthase also known as CTPS or the CTP protein is an enzyme involved in pyrimidine metabolism. It catalyzes the conversion of UTP to CTP an essential step in nucleotide biosynthesis. The enzyme exists in two isoforms CTPS1 and CTPS2 with both found in various tissues but CTPS2 has a higher expression in the brain. CTP synthase has a molecular mass of roughly 70 kDa. CTPS can assemble into filamentous structures under certain conditions influencing its enzymatic activity. These filaments sometimes referred to as cytoophidia are observed in many types of cells.

Biological function summary

CTP synthase plays a significant role in the regulation of nucleotide pools. The enzyme ensures the balance of CTP levels necessary for DNA RNA and phospholipid synthesis acting as a rate-limiting step in these processes. CTPS is part of larger enzymatic complexes that aid in cellular response to nutrient availability and DNA damage. Allosteric regulation and product feedback inhibition modulate CTP synthase activity maintaining cellular homeostasis and a proper supply of CTP for various biosynthetic pathways.

Pathways

CTP synthase is an important component in the pyrimidine biosynthesis pathway essential for nucleic acid and lipid metabolism. The enzyme's activity is tightly linked to the salvage pathways collaborating with enzymes like ribonucleotide reductase to regulate deoxyribonucleotide levels for DNA replication and repair. These interactions highlight the enzyme's involvement in cellular proliferation and response to metabolic stresses underpinning its regulatory function in metabolic pathways.

CTP synthase activity disorders relate to cancer and neurodegeneration. Dysregulation of CTPS is associated with increased proliferation seen in certain cancers given its role in nucleotide biosynthesis. Research also indicates a link between CTPS activity and neurodegenerative diseases as nucleotide imbalance affects neural function. Additionally interactions with proteins involved in cell cycle control and apoptosis such as p53 and Rb further highlight CTPS's role in disease etiology influencing therapeutic strategies targeting these diverse conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

CTP synthase involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids (PubMed : 16179339, PubMed : 17189248, PubMed : 17463002, PubMed : 24870241, PubMed : 28459447, PubMed : 34583994). Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen (PubMed : 16179339, PubMed : 24870241, PubMed : 28459447, PubMed : 34583994). CTPS1 CTP synthase activity plays a crucial role in the proliferation of activated lymphocytes and immunity; additional CTP being required to meet increased demand for DNA, RNA and lipid membrane biosynthesis in proliferating lymphocytes (PubMed : 24870241, PubMed : 8530356). In addition to CTP synthase activity, also acts as a protein deamidase that catalyzes the side chain deamidation of specific asparagine residues of proteins to aspartate (PubMed : 40240600). Acts as a negative regulator of innate immunity by mediating deamidation of 'Asn-85' of IRF3, preventing IRF3 from binding DNA (By similarity). Facilitates chromatin relaxation in response to DNA damage by mediating deamidation of 'Asn-76' and 'Asn-77' of histone H1, thereby promoting subsequent acetylation of histone H1 at 'Lys-75' (H1K75ac), increasing chromatin accessibility to facilitate the recruitment of DNA repair proteins (PubMed : 40240600).

See full target information CTPS1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40957650

2025

CTPS2 regulates CTP synthetase activity by interacting with CTPS1.

Applications

Unspecified application

Species

Unspecified reactive species

Norbert Minet,Benoît Heid-Picard,Vanessa Masson,Damarys Loew,Emmanuel Martin,Sylvain Latour

Redox biology 84:103649 PubMed40446642

2025

PRPS activity tunes redox homeostasis in Myc-driven lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Austin C MacMillan,Bibek Karki,Juechen Yang,Karmela R Gertz,Samantha Zumwalde,Jay G Patel,Maria F Czyzyk-Krzeska,Jarek Meller,John T Cunningham

Nature communications 15:1982 PubMed38438357

2024

Inactivation of cytidine triphosphate synthase 1 prevents fatal auto-immunity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Soudais,Romane Schaus,Camille Bachelet,Norbert Minet,Sara Mouasni,Cécile Garcin,Caique Lopes Souza,Pierre David,Clara Cousu,Hélène Asnagli,Andrew Parker,Paul Palmquist-Gomes,Fernando E Sepulveda,Sébastien Storck,Sigolène M Meilhac,Alain Fischer,Emmanuel Martin,Sylvain Latour

Life science alliance 6: PubMed37348953

2023

Differential roles of CTP synthetases CTPS1 and CTPS2 in cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Norbert Minet,Anne-Claire Boschat,Rebecca Lane,David Laughton,Philip Beer,Hélène Asnagli,Claire Soudais,Tim Bourne,Alain Fischer,Emmanuel Martin,Sylvain Latour

Nature communications 6:7200 PubMed26007203

2015

Translational regulation shapes the molecular landscape of complex disease phenotypes.

Applications

Species

Unspecified reactive species

Sebastian Schafer,Eleonora Adami,Matthias Heinig,Katharina E Costa Rodrigues,Franziska Kreuchwig,Jan Silhavy,Sebastiaan van Heesch,Deimante Simaite,Nikolaus Rajewsky,Edwin Cuppen,Michal Pravenec,Martin Vingron,Stuart A Cook,Norbert Hubner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CTP synthase/CTPS antibody [EPR8086(B)]

Flow Cytometry (Intracellular) - Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB133743)

Western blot - Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB133743)

OI-RD Scanning - Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB133743)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reagents, Controls & Accessories

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise