AB211639

Anti-CTP synthase/CTPS antibody [EPR8086(B)] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal CTP synthase/CTPS antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CTPS, CTPS1, CTP synthase 1, CTP synthetase 1, UTP--ammonia ligase 1

2 Images

Flow Cytometry (Intracellular) - Anti-CTP synthase/CTPS antibody [EPR8086(B)] - BSA and Azide free (AB211639)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CTP synthase/CTPS antibody [EPR8086(B)] - BSA and Azide free (AB211639)

Intracellular Flow Cytometry analysis of permeabilized Raji cells labelling CTP synthase/CTPS with ab133743 at 1/10 (red), with rabbit IgG negative control (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133743).

OI-RD Scanning - Anti-CTP synthase/CTPS antibody [EPR8086(B)] - BSA and Azide free (AB211639)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CTP synthase/CTPS antibody [EPR8086(B)] - BSA and Azide free (AB211639)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8086(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

ab211639 is the carrier-free version of ab133743.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTP synthase also known as CTPS or the CTP protein is an enzyme involved in pyrimidine metabolism. It catalyzes the conversion of UTP to CTP an essential step in nucleotide biosynthesis. The enzyme exists in two isoforms CTPS1 and CTPS2 with both found in various tissues but CTPS2 has a higher expression in the brain. CTP synthase has a molecular mass of roughly 70 kDa. CTPS can assemble into filamentous structures under certain conditions influencing its enzymatic activity. These filaments sometimes referred to as cytoophidia are observed in many types of cells.

Biological function summary

CTP synthase plays a significant role in the regulation of nucleotide pools. The enzyme ensures the balance of CTP levels necessary for DNA RNA and phospholipid synthesis acting as a rate-limiting step in these processes. CTPS is part of larger enzymatic complexes that aid in cellular response to nutrient availability and DNA damage. Allosteric regulation and product feedback inhibition modulate CTP synthase activity maintaining cellular homeostasis and a proper supply of CTP for various biosynthetic pathways.

Pathways

CTP synthase is an important component in the pyrimidine biosynthesis pathway essential for nucleic acid and lipid metabolism. The enzyme's activity is tightly linked to the salvage pathways collaborating with enzymes like ribonucleotide reductase to regulate deoxyribonucleotide levels for DNA replication and repair. These interactions highlight the enzyme's involvement in cellular proliferation and response to metabolic stresses underpinning its regulatory function in metabolic pathways.

CTP synthase activity disorders relate to cancer and neurodegeneration. Dysregulation of CTPS is associated with increased proliferation seen in certain cancers given its role in nucleotide biosynthesis. Research also indicates a link between CTPS activity and neurodegenerative diseases as nucleotide imbalance affects neural function. Additionally interactions with proteins involved in cell cycle control and apoptosis such as p53 and Rb further highlight CTPS's role in disease etiology influencing therapeutic strategies targeting these diverse conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity.

See full target information CTPS1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Digestive diseases and sciences 70:1587 PubMed40057665

2025

Correction to: Chromosome 12 Open Reading Frame 49 Promotes Tumor Growth and Predicts Poor Prognosis in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Tao,Jia Luo,Hongyi Zhu,Yi Chu,Lei Pei

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CTP synthase/CTPS antibody [EPR8086(B)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-CTP synthase/CTPS antibody [EPR8086(B)] - BSA and Azide free (AB211639)

OI-RD Scanning - Anti-CTP synthase/CTPS antibody [EPR8086(B)] - BSA and Azide free (AB211639)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Product promise