AB190462

Anti-CTPS2 antibody - C-terminal

Name: Anti-CTPS2 antibody - C-terminal (ab190462) | Abcam
Brand: Abcam Limited
SKU: AB190462
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal CTPS2 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CTPS2 aa 500 to C-terminus.

View Alternative Names

CTP synthase 2, CTP synthetase 2, UTP--ammonia ligase 2, CTPS2

2 Images

Supplier Data

Western blot - Anti-CTPS2 antibody - C-terminal (AB190462)

All lanes:

Western blot - Anti-CTPS2 antibody - C-terminal (ab190462) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate in NETN buffer at 50 µg

Lane 2:

293T whole cell lysate in NETN buffer at 50 µg

Lane 3:

Jurkat whole cell lysate in NETN buffer at 50 µg

Predicted band size: 66 kDa

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Exposure time: 3min

Supplier Data

Immunoprecipitation - Anti-CTPS2 antibody - C-terminal (AB190462)

Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 1 mg per IP reaction; 20% of IP loaded) labeling CTPS2 using ab190462 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated CTPS2, ab190462 was used at 1 μg/ml. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-CTPS2 antibody - C-terminal (ab190462)

Predicted band size: 66 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CTPS2 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NRF8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTPS2 or CTP synthase 2 plays a role in pyrimidine biosynthesis by converting UTP to CTP an essential step in nucleotide metabolism. This enzyme has a mass of approximately 64 kDa. CTPS2 is expressed in various tissues with notable expression in immune-related cells and the liver. Its activity contributes to maintaining a balance between the pyrimidine nucleotides necessary for DNA and RNA synthesis.

Biological function summary

CTPS2 is involved in regulating nucleotide pools which is critical for cell proliferation and differentiation. This protein does not typically form part of a larger complex but acts as a homotetramer. CTPS2's function ensures that cells have enough cytidine triphosphate (CTP) for various cellular processes including lipid synthesis and glycosylation reactions vital for maintaining cell membrane integrity and glycoprotein functions.

Pathways

CTPS2 plays a significant role in the pyrimidine metabolic pathway. It is directly involved in the de novo synthesis of CTP. This pathway links to the salvage pathway where it interacts indirectly with proteins involved in nucleotide salvage like uridine-cytidine kinase (UCK) which phosphorylates nucleosides to monophosphate nucleotides. Through these pathways CTPS2 influences DNA replication and cell growth.

CTPS2 has connections to cancer and immunodeficiency disorders. Overexpression of CTPS2 is seen in certain cancers where it may contribute to unchecked cell division. On the other hand impaired CTPS2 function can result in reduced immune response affecting T cell proliferation. The protein is also linked to ribonucleotide reductase M2 (RRM2) through pathways impacting the balance of deoxyribonucleotides in rapidly dividing cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.

See full target information CTPS2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 6: PubMed37348953

2023

Differential roles of CTP synthetases CTPS1 and CTPS2 in cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Norbert Minet,Anne-Claire Boschat,Rebecca Lane,David Laughton,Philip Beer,Hélène Asnagli,Claire Soudais,Tim Bourne,Alain Fischer,Emmanuel Martin,Sylvain Latour

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CTPS2 antibody - C-terminal

Western blot - Anti-CTPS2 antibody - C-terminal (AB190462)

Immunoprecipitation - Anti-CTPS2 antibody - C-terminal (AB190462)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise