Rabbit Polyclonal CTR9 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CTR9 aa 1100 to C-terminus.
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Publications
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- Scientific reports 13:198852023SETD5 regulates the OGT-catalyzed O-GlcNAcylation of RNA polymerase II, which is involved in the stemness of colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye In Cho,Sora Jo,Min Seong Kim,Han Byeol Kim,Xingzhe Liu,Yanhua Xuan,Jin Won Cho,Yeun Kyu JangPubMed 37963940
- Cell systems 1:141-512015Systems Analyses Reveal Shared and Diverse Attributes of Oct4 Regulation in Pluripotent Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Ding et. al.PubMed 27135800
- Developmental biology 383:15-272013CTR9/PAF1c regulates molecular lineage identity, histone H3K36 trimethylation and genomic imprinting during preimplantation development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun Zhang et. al.PubMed 24036311
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CTR9 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PD62
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Predicted |
| Chimpanzee | Predicted |
| Chinese hamster | Predicted |
| Elephant | Predicted |
| Gorilla | Predicted |
| Orangutan | Predicted |
| Rhesus monkey | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes Antigen retrieval is recommended: retrieval with citrate buffer will enhance staining.Likely to work with frozen sections |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Antigen retrieval is recommended: retrieval with citrate buffer will enhance staining.Likely to work with frozen sections |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
Target data
Function
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3 (By similarity).
Alternative names
KIAA0155, SH2BP1, CTR9, RNA polymerase-associated protein CTR9 homolog, SH2 domain-binding protein 1
Recommended products
Rabbit Polyclonal CTR9 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CTR9 aa 1100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CTR9 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PD62
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibody was affinity purified using an epitope specific to CTR9 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CTR9 often referred to as a component of the polymerase-associated factor 1 complex (PAF1C) is a 133 kDa protein. It plays a mechanical role in transcription regulation functioning through interactions with RNA polymerase II. CTR9 is widely expressed across multiple tissues including the heart and lungs. While there is limited tissue specificity it maintains a consistent expression in these regions making it an interesting target for further study.
- Biological function summary
CTR9 participates in chromatin modification and transcription elongation within PAF1C. This complex influences various nuclear processes including the modification of histones and regulation of gene expression. CTR9's involvement assists in facilitating the transition between transcriptional initiation and elongation phases aiding in the overall gene expression.
- Pathways
CTR9 integrates into the transcriptional regulation pathway and the NOTCH signaling pathway. In these pathways CTR9 acts by interacting with other components such as components of RNA polymerase II ensuring proper gene transcription and regulation. Its interactions with NOTCH receptors highlight its involvement in processes like cell differentiation and development.
- Associated diseases and disorders
CTR9 is implicated in colorectal and breast cancer. Dysregulation of its function often contributes to the progression of these malignant conditions. Proteins such as BRCA1 connect with CTR9 through pathways implicated in breast cancer demonstrating its vital role in maintaining cellular homeostasis and genomic integrity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTR9 antibody (ab84487)
ab84487 at 1/250 dilution staining formalin-fixed, paraffin-embedded section of human breast carcinoma by immunohistochemistry.
Detection: DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTR9 antibody (ab84487)
ab 84487 at 1/250 dilution staining formalin-fixed, paraffin-embedded section of human breast carcinoma by immunohistochemistry.
Detection: DAB staining
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTR9 antibody (ab84487)
ab 84487 at 1/250 dilution staining formalin-fixed, paraffin-embedded section of mouse teratoma by immunohistochemistry.
Detection: DAB staining
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