AB36871

Anti-CTRP4 antibody

Name: Anti-CTRP4 antibody (ab36871) | Abcam
Brand: Abcam Limited
SKU: AB36871

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Polyclonal CTRP4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human C1QTNF4.

View Alternative Names

CTRP4, C1QTNF4, Complement C1q tumor necrosis factor-related protein 4, C1q/TNF-related protein 4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CTRP4 antibody (AB36871)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CTRP4 antibody (AB36871)

Immunofluorescence of CTRP4 in Rat Brain cells using ab36871 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP4 antibody (AB36871)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP4 antibody (AB36871)

ab36891 at 10μg/ml staining CTRP4 in rat brain by IHC

Unknown

Western blot - Anti-CTRP4 antibody (AB36871)

Lanes read from left to right 1, 2 and 3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size.

Lane 1:

Western blot - Anti-CTRP4 antibody (ab36871) at 1 µg/mL

Lane 2:

Western blot - Anti-CTRP4 antibody (ab36871) at 2 µg/mL

Lane 3:

Western blot - Anti-CTRP4 antibody (ab36871) at 4 µg/mL

All lanes:

Rat brain cell lysate at 15 µg/mL

Secondary

All lanes:

anti-rabbit IgG HRP at 1/10000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human C1QTNF4. The exact immunogen used to generate this antibody is proprietary information.

Q9BXJ3

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTRP4 also known as C1q and tumor necrosis factor-related protein 4 is a member of the C1q/TNF-related protein family. It has a molecular weight of approximately 31 kDa. The protein is expressed in various tissues including adipose tissue and the liver where it performs regulatory functions by interacting with other cellular components. Its presence in these tissues suggests roles in metabolism and inflammation.

Biological function summary

CTRP4 functions as a modulator of immune responses and energy balance. It is involved in forming complexes that can influence inflammatory pathways playing a part in controlling cytokine production. As a member of the CTRP family CTRP4 shares structural homology that allows it to interact with receptors implicated in metabolic and immune processes. This connection points to its role in maintaining homeostasis.

Pathways

CTRP4 participates in metabolic and inflammatory signaling networks. It engages in the AMPK signaling pathway which is critical for energy regulation. Within this context CTRP4 acts together with proteins like adiponectin to modulate glucose uptake and lipid metabolism. In the inflammatory landscape CTRP4 influences the MAPK signaling pathway affecting cellular responses to various stressors and regulatory signals.

CTRP4 has associations with obesity and type 2 diabetes. Its role in these conditions links to its function in metabolic regulation and inflammation control. For instance CTRP4 modulates inflammatory profiles connecting it with proteins such as TNF-alpha in the context of metabolic syndrome. Research continues to explore its potential as a therapeutic target given its involvement in these significant health challenges.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May be involved in the regulation of the inflammatory network. Its role as pro- or anti-inflammatory seems to be context dependent (PubMed : 21658842, PubMed : 27086950). Seems to have some role in regulating food intake and energy balance when administered in the brain. This effect is sustained over a two-day period, and it is accompanied by decreased expression of orexigenic neuropeptides in the hypothalamus 3 hours post-injection (By similarity).

See full target information C1QTNF4

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 296:100513 PubMed33676896

2021

Nucleolin acts as the receptor for C1QTNF4 and supports C1QTNF4-mediated innate immunity modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Susan K Vester,Rebecca L Beavil,Steven Lynham,Andrew J Beavil,Deborah S Cunninghame Graham,James M McDonnell,Timothy J Vyse

The Journal of experimental medicine 216:1733-1748 PubMed31189656

2019

CXCR4 regulates development in mouse and human hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hironori Bando,Ariel Pradipta,Shiroh Iwanaga,Toru Okamoto,Daisuke Okuzaki,Shun Tanaka,Joel Vega-Rodríguez,Youngae Lee,Ji Su Ma,Naoya Sakaguchi,Akira Soga,Shinya Fukumoto,Miwa Sasai,Yoshiharu Matsuura,Masao Yuda,Marcelo Jacobs-Lorena,Masahiro Yamamoto

Biochemical and biophysical research communications 512:224-229 PubMed30885436

2019

Ctrp4, a new adipokine, promotes the differentiation of osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Li,Jieheng Wu,Wenjin Xi,Xu Chen,Wei Wang,Tianze Zhang,Angang Yang,Tao Wang

The Journal of biological chemistry 289:4055-69 PubMed24366864

2013

C1q/TNF-related protein 4 (CTRP4) is a unique secreted protein with two tandem C1q domains that functions in the hypothalamus to modulate food intake and body weight.

Applications

Species

Mouse

Mardi S Byerly,Pia S Petersen,Santosh Ramamurthy,Marcus M Seldin,Xia Lei,Elayne Provost,Zhikui Wei,Gabriele V Ronnett,G William Wong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CTRP4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CTRP4 antibody (AB36871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP4 antibody (AB36871)

Western blot - Anti-CTRP4 antibody (AB36871)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise