AB300583

Anti-CTRP6 antibody [EPR25750-35]

20ul selling size
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal CTRP6 antibody. Suitable for WB, IP and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CTRP6, UNQ581/PRO1151, C1QTNF6, Complement C1q tumor necrosis factor-related protein 6

3 Images

Supplier Data

Immunoprecipitation - Anti-CTRP6 antibody [EPR25750-35] (AB300583)

CTRP6 was immunoprecipitated from 0.35 mg human placenta tissue lysate with ab300583 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300583 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CTRP6 antibody [EPR25750-35] (ab300583) at 1/30 dilution

Lane 1:

Human placenta tissue lysate (Inset) at 10 µg

Lane 2:

ab300583 IP in Human placenta tissue lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300583 in human placenta tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CTRP6 antibody [EPR25750-35] (AB300583)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : skeletal muscle (PMID : 28726640)

All lanes:

Western blot - Anti-CTRP6 antibody [EPR25750-35] (ab300583) at 1/1000 dilution

Lane 1:

human placenta tissue lysate 40 µg

Lane 2:

human skeletal muscle tissue lysate 40 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 30 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CTRP6 antibody [EPR25750-35] (AB300583)

Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human CTRP8.

All lanes:

Western blot - Anti-CTRP6 antibody [EPR25750-35] (ab300583) at 1/1000 dilution

Lane 1:

Human CTRP6 recombinant protein 5 ng

Lane 2:

His-tagged human CTRP8 recombinant protein 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

Exposure time: 3.25s

Carrier free

Anti-CTRP6 antibody [EPR25750-35] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25750-35

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTRP6 also known as C1q/TNF-related protein 6 is a secreted protein that belongs to the C1q/TNF-related protein family. It has a molecular mass of approximately 37 kDa. Expression of CTRP6 occurs in various tissues including adipose tissue skeletal muscle and liver. Its structural components include a collagen-like domain and a C1q globular domain which are characteristic of proteins involved in immune responses and metabolic regulation.

Biological function summary

CTRP6 plays a significant role in modulating inflammatory responses and metabolism. It acts by influencing the secretion and activity of cytokines such as IL-6 and TNF-alpha. Although not frequently noted as part of a multi-protein complex CTRP6 interacts closely with signaling molecules to achieve its functions. Studies suggest its involvement in maintaining metabolic balance and regulating insulin sensitivity key processes in various physiological states.

Pathways

It interacts with several metabolic and inflammatory signaling cascades. This protein mediates effects through pathways like the AMPK signaling pathway which plays an important role in energy balance and metabolic stress responses. CTRP6 is also linked to the TLR4 signaling pathway involved in immune activation and inflammation. These pathways involve interactions with various proteins such as AMPK and TLR4 receptors highlighting its integrative role in cell signaling networks.

CTRP6 shows an association with conditions like obesity and type 2 diabetes. These diseases link to its role in regulating metabolic and inflammatory responses. Dysfunctional CTRP6 expression or activity has been observed in individuals with these metabolic disorders. Additionally CTRP6 relates to the expression and regulation of proteins such as adiponectin and TNF-alpha which are also involved in these disease pathways. These connections emphasize its potential as a therapeutic target for metabolic interventions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information C1QTNF6

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:409 PubMed40457410

2025

Targeted tumor cell-intrinsic CTRP6 biomimetic codelivery synergistically amplifies ferroptosis and immune activation to boost anti-PD-L1 immunotherapy efficacy in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Songhua Cai,Jing Huang,Hongjie Fan,Zhilin Sui,Chujian Huang,Youjun Deng,Ran Jia,Lixu Wang,Kai Ma,Xiaotong Guo,Jie He,Baihua Zhang,Zhentao Yu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CTRP6 antibody [EPR25750-35]

Immunoprecipitation - Anti-CTRP6 antibody [EPR25750-35] (AB300583)

Western blot - Anti-CTRP6 antibody [EPR25750-35] (AB300583)

Western blot - Anti-CTRP6 antibody [EPR25750-35] (AB300583)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise